Crystallization (Comment) | Organism |
---|---|
in complex with diphosphomevalonate and Mg2+, diffraction to 2.5 A resolution. Diphosphomevalonate functions as a partial bisubstrate analogue and elicits a ternary-complex like form of the enzyme | Streptococcus pneumoniae |
purified enzyme in complex with 5-diphosphomevalonate and Mg2+ bound to the active cleft, sitting drop vapour diffusion method, 0.002 ml of 6,0 mg/ml protein in 50 mM HEPES, pH 7.5, 50 mM KCl, 1 mM DTT, 10 mM adenosine 5'-(beta,gamma-imino)triphosphate, 12 mM MgCl2, and 0.5 mM diphosphomevalonate is mixed with 0.002 ml of precipitatnt solution containing 40% v/v PEG 400, and 200 mM sodium formate, at room temperature, X-ray diffraction structure determination and anaylsis at 2.5 A resolution, single-wavelength anomalous diffraction method | Streptococcus pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-diphosphomevalonate | allosteric and potent inhibition, the molecule functions as a partial bisubstrate analogue that elicits a ternary-complex-like form of the enzyme, binding structure, overview | Streptococcus pneumoniae | |
diphosphomevalonate | functions as a partial bisubstrate analogue and elicits a ternary-complex like form of the enzyme | Streptococcus pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | binding structure, overview | Streptococcus pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-mevalonate | Streptococcus pneumoniae | the enzyme catalyzes a step in the isoprenoid biosynthetic pathway via the essential intermediate 5-diphosphomevalonate | ADP + (R)-5-phosphomevalonate | - |
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Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-mevalonate | the enzyme catalyzes a step in the isoprenoid biosynthetic pathway via the essential intermediate 5-diphosphomevalonate | Streptococcus pneumoniae | ADP + (R)-5-phosphomevalonate | - |
? | |
ATP + (R)-mevalonate | substrate binding structure, overview | Streptococcus pneumoniae | ADP + (R)-5-phosphomevalonate | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Streptococcus pneumoniae |