Cloned (Comment) | Organism |
---|---|
native and mutant enzymes expressed as His-tag fusion proteins in Escherichia coli BL21(DE3) | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
H20K | expressed in inclusion bodies that can be solubilized in 8 M urea, refolding to a soluble protein was unsuccessful indicating irreversible structural changes | Rattus norvegicus |
H20L | no significant changes in secondary structure, increased Km for both substrates | Rattus norvegicus |
H20Y | no significant changes in secondary structure, increased Km for both substrates | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
farnesyl diphosphate | competitive with ATP | Rattus norvegicus | |
geranyl diphosphate | competitive with ATP | Rattus norvegicus | |
geranylgeranyl diphosphate | competitive with ATP | Rattus norvegicus | |
prenyl phosphates | competitive with ATP | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
(R)-mevalonate | native enzyme, pH 7.5, 25°C | Rattus norvegicus | |
0.616 | - |
(R)-mevalonate | H20L mutant enzyme, pH 7.5, 25°C | Rattus norvegicus | |
0.953 | - |
ATP | native enzyme, pH 7.5, 25°C | Rattus norvegicus | |
1.81 | - |
ATP | H20L mutant enzyme, pH 7.5, 25°C | Rattus norvegicus | |
2.16 | - |
(R)-mevalonate | H20Y mutant enzyme, pH 7.5, 25°C | Rattus norvegicus | |
2.7 | - |
ATP | H20Y mutant enzyme enzyme, pH 7.5, 25°C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
x * 42000, SDS-PAGE, His-tag protein | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-mevalonate | Rattus norvegicus | involved in cholesterol, steroid hormones und terpenoids synthesis | ADP + (R)-5-phosphomevalonate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant proteins using His-tag | Rattus norvegicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
32.7 | - |
purified His-tag protein, pH 7.5, 25°C | Rattus norvegicus |
Storage Stability | Organism |
---|---|
4°C, 5% glycerol, 5 mM 2-mercaptoethanol, stable for more than 1 month | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-mevalonate | - |
Rattus norvegicus | ADP + (R)-5-phosphomevalonate | - |
? | |
ATP + (R)-mevalonate | involved in cholesterol, steroid hormones und terpenoids synthesis | Rattus norvegicus | ADP + (R)-5-phosphomevalonate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, SDS-PAGE, His-tag protein | Rattus norvegicus |