Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.36 extracted from

  • Chu, X.; Li, D.
    Expression, purification, and characterization of His20 mutants of rat mevalonate kinase (2003), Protein Expr. Purif., 32, 75-82.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
native and mutant enzymes expressed as His-tag fusion proteins in Escherichia coli BL21(DE3) Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
H20K expressed in inclusion bodies that can be solubilized in 8 M urea, refolding to a soluble protein was unsuccessful indicating irreversible structural changes Rattus norvegicus
H20L no significant changes in secondary structure, increased Km for both substrates Rattus norvegicus
H20Y no significant changes in secondary structure, increased Km for both substrates Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
farnesyl diphosphate competitive with ATP Rattus norvegicus
geranyl diphosphate competitive with ATP Rattus norvegicus
geranylgeranyl diphosphate competitive with ATP Rattus norvegicus
prenyl phosphates competitive with ATP Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
(R)-mevalonate native enzyme, pH 7.5, 25°C Rattus norvegicus
0.616
-
(R)-mevalonate H20L mutant enzyme, pH 7.5, 25°C Rattus norvegicus
0.953
-
ATP native enzyme, pH 7.5, 25°C Rattus norvegicus
1.81
-
ATP H20L mutant enzyme, pH 7.5, 25°C Rattus norvegicus
2.16
-
(R)-mevalonate H20Y mutant enzyme, pH 7.5, 25°C Rattus norvegicus
2.7
-
ATP H20Y mutant enzyme enzyme, pH 7.5, 25°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Rattus norvegicus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
x * 42000, SDS-PAGE, His-tag protein Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (R)-mevalonate Rattus norvegicus involved in cholesterol, steroid hormones und terpenoids synthesis ADP + (R)-5-phosphomevalonate
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant proteins using His-tag Rattus norvegicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
32.7
-
purified His-tag protein, pH 7.5, 25°C Rattus norvegicus

Storage Stability

Storage Stability Organism
4°C, 5% glycerol, 5 mM 2-mercaptoethanol, stable for more than 1 month Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-mevalonate
-
Rattus norvegicus ADP + (R)-5-phosphomevalonate
-
?
ATP + (R)-mevalonate involved in cholesterol, steroid hormones und terpenoids synthesis Rattus norvegicus ADP + (R)-5-phosphomevalonate
-
?

Subunits

Subunits Comment Organism
? x * 42000, SDS-PAGE, His-tag protein Rattus norvegicus