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Literature summary for 2.7.1.36 extracted from
- Overexpression, purification, and characterization of the thermostable mevalonate kinase from Methanococcus jannaschii (1999), Protein Expr. Purif., 17, 33-40.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Methanocaldococcus jannaschii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| farnesyl diphosphate | 0.01 mM, 65% inhibition | Methanocaldococcus jannaschii |  |
| geranyl diphosphate | 0.01 mM, 55% inhibition | Methanocaldococcus jannaschii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.069 | - |
(R,S)-mevalonate | pH 8.0, 70°C, recombinant mevalonate kinase | Methanocaldococcus jannaschii | |
| 0.092 | - |
ATP | pH 8.0, 70°C, recombinant mevalonate kinase | Methanocaldococcus jannaschii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
2 * 37000, SDS-PAGE | Methanocaldococcus jannaschii |
| 68000 | - |
gel filtration | Methanocaldococcus jannaschii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + mevalonate | Methanocaldococcus jannaschii | involved in mevalonate pathway of terpenoid biosynthesis | ADP + phosphomevalonate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanocaldococcus jannaschii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant mevalonate kinase, 70°C heat treatment, Talon metal-affinity resin | Methanocaldococcus jannaschii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 387 | - |
recombinant mevalonate kinase | Methanocaldococcus jannaschii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + mevalonate | - |
Methanocaldococcus jannaschii | ADP + phosphomevalonate | - |
? | |
| ATP + mevalonate | involved in mevalonate pathway of terpenoid biosynthesis | Methanocaldococcus jannaschii | ADP + phosphomevalonate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 37000, SDS-PAGE | Methanocaldococcus jannaschii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 75 | - |
Methanocaldococcus jannaschii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 90 | approx. 25% of maximal activity at 40°C and 90°C, respectively | Methanocaldococcus jannaschii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
no loss of activity after 24 h | Methanocaldococcus jannaschii |
| 90 | - |
50% loss of activity after 15 min | Methanocaldococcus jannaschii |
| 100 | - |
50% loss of activity after 5 min | Methanocaldococcus jannaschii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.5 | - |
Methanocaldococcus jannaschii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | at least 60% of maximal activity between pH 5.0 and 7.0 | Methanocaldococcus jannaschii |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Methanocaldococcus jannaschii | MonoP chromatography | - |
7.8 |
| Methanocaldococcus jannaschii | calculated from amino acid sequence | - |
8.4 |
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