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Literature summary for 2.7.1.35 extracted from

  • Deka, G.; Kalyani, J.N.; Jahangir, F.B.; Sabharwal, P.; Savithri, H.S.; Murthy, M.R.N.
    Structural and functional studies on Salmonella typhimurium pyridoxal kinase the first structural evidence for the formation of Schiff base with the substrate (2019), FEBS J., 286, 3684-3700 .
    View publication on PubMed
Search for more literature for 2.7.1.35

Crystallization (Commentary)

Crystallization (Comment) Organism
unliganded StPLK (P212121) and two different tetragonal (P43212) crystal forms (form I and form II) of its complex with PL, ADP, and Mg2+, X-ray diffraction structure determination and analysis at 2.2-2.6 A resolution, modelling Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

Inhibitors Comment Organism Structure
ADP at high concentrations, product inhibition Salmonella enterica subsp. enterica serovar Typhimurium
pyridoxal at high concentrations, substrate inhibition Salmonella enterica subsp. enterica serovar Typhimurium
pyridoxal 5'-phosphate at high concentrations, product inhibition Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state kinetics Salmonella enterica subsp. enterica serovar Typhimurium
0.044
-
 pyridoxal pH 6.6, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
0.06
-
 pyridoxamine pH 8.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
0.1
-
 ATP with pyridoxal, pH 6.6, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
0.12
-
 ATP with pyridoxamine, pH 8.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, Mg2+ ion can be located near the bound phosphate, it is coordinated with O3 of the ADP beta-phosphate and four water molecules Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + pyridoxal Salmonella enterica subsp. enterica serovar Typhimurium
-
 ADP + pyridoxal 5'-phosphate
-
 ?
ATP + pyridoxamine Salmonella enterica subsp. enterica serovar Typhimurium
-
 ADP + pyridoxamine 5'-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium A0A0F7J8S0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pyridoxal
-
 Salmonella enterica subsp. enterica serovar Typhimurium ADP + pyridoxal 5'-phosphate
-
 ?
ATP + pyridoxal preferred substrate Salmonella enterica subsp. enterica serovar Typhimurium ADP + pyridoxal 5'-phosphate
-
 ?
ATP + pyridoxamine
-
 Salmonella enterica subsp. enterica serovar Typhimurium ADP + pyridoxamine 5'-phosphate
-
 ?
additional information substrate binding strutures analysis from cyrstal structure of the enzyme-ligand complex, overview. ATP is covalently attached to Lys233 via a Schiff base linkage. In StPLK, the alpha-phosphate of ADP interacts with Ser201, Thr235, and nearby water molecule. The beta-phosphate of ADP interacts with Gly236, Asn164, one Mg2+ ion, and a few water molecules. Pyridoxal is covalently attached to an active site lysine residue (Lys233) forming a Schiff base, as well as to ADP, and a Mg2+ ion. Active site structure. Structure comparisons with other pyridoxal kinases from other species Salmonella enterica subsp. enterica serovar Typhimurium ?
-
-

Synonyms

Synonyms Comment Organism
PLK
-
 Salmonella enterica subsp. enterica serovar Typhimurium
StPLK
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 activity range Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.097
-
 pyridoxamine pH 8.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
0.097
-
 ATP with pyridoxamine, pH 8.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
0.107
-
 pyridoxal pH 6.6, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
0.107
-
 ATP with pyridoxal, pH 6.6, 37°C Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2 6.6 with substrate pyridoxal Salmonella enterica subsp. enterica serovar Typhimurium
8.5
-
 with substrate pyridoxamine Salmonella enterica subsp. enterica serovar Typhimurium

General Information

General Information Comment Organism
additional information comparison of the structure of apo StPLK with its ligand-bound forms Salmonella enterica subsp. enterica serovar Typhimurium

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.81
-
 ATP with pyridoxamine, pH 8.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.07
-
 ATP with pyridoxal, pH 6.6, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.62
-
 pyridoxamine pH 8.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.43
-
 pyridoxal pH 6.6, 37°C Salmonella enterica subsp. enterica serovar Typhimurium