Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Chloroquine | about 40% inhibition at 1 mM | Plasmodium vivax | |
additional information | not inhibited by chloroquine | Homo sapiens | |
additional information | not inhibited by chloroquine | Trypanosoma cruzi | |
primaquine | competitive inhibition, complete inhibition at 0.75 mM | Homo sapiens | |
primaquine | competitive inhibition, complete inhibition at 1 mM | Plasmodium vivax | |
primaquine | competitive inhibition, about 70% inhibition at 1 mM | Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00122 | - |
pyridoxal | in the presence of K+, at pH 7.0 and 30°C | Homo sapiens | |
0.0153 | - |
pyridoxal | in the presence of K+, at pH 7.0 and 30°C | Plasmodium vivax | |
0.0216 | - |
pyridoxal | in the presence of K+, at pH 7.0 and 30°C | Trypanosoma cruzi | |
0.0739 | - |
pyridoxal | in the presence of Na+, at pH 7.0 and 30°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | activates | Homo sapiens | |
K+ | activates | Trypanosoma cruzi | |
K+ | activates | Plasmodium vivax | |
Na+ | activates | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
x * 35000, SDS-PAGE | Homo sapiens |
35000 | - |
x * 35000, SDS-PAGE | Trypanosoma cruzi |
35000 | - |
x * 35000, SDS-PAGE | Plasmodium vivax |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyridoxal | Homo sapiens | - |
ADP + pyridoxal 5'-phosphate | - |
? | |
ATP + pyridoxal | Trypanosoma cruzi | - |
ADP + pyridoxal 5'-phosphate | - |
? | |
ATP + pyridoxal | Plasmodium vivax | - |
ADP + pyridoxal 5'-phosphate | - |
? | |
ATP + pyridoxal | Plasmodium vivax Salvador I | - |
ADP + pyridoxal 5'-phosphate | - |
? | |
ATP + pyridoxal | Trypanosoma cruzi CL Brener | - |
ADP + pyridoxal 5'-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Plasmodium vivax | A5K1Z8 | - |
- |
Plasmodium vivax Salvador I | A5K1Z8 | - |
- |
Trypanosoma cruzi | Q4D946 | - |
- |
Trypanosoma cruzi CL Brener | Q4D946 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Homo sapiens |
Ni-NTA agarose column chromatography | Trypanosoma cruzi |
Ni-NTA agarose column chromatography | Plasmodium vivax |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lung | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyridoxal | - |
Homo sapiens | ADP + pyridoxal 5'-phosphate | - |
? | |
ATP + pyridoxal | - |
Trypanosoma cruzi | ADP + pyridoxal 5'-phosphate | - |
? | |
ATP + pyridoxal | - |
Plasmodium vivax | ADP + pyridoxal 5'-phosphate | - |
? | |
ATP + pyridoxal | - |
Plasmodium vivax Salvador I | ADP + pyridoxal 5'-phosphate | - |
? | |
ATP + pyridoxal | - |
Trypanosoma cruzi CL Brener | ADP + pyridoxal 5'-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, SDS-PAGE | Homo sapiens |
? | x * 35000, SDS-PAGE | Trypanosoma cruzi |
? | x * 35000, SDS-PAGE | Plasmodium vivax |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
pyridoxal | in the presence of K+, at pH 7.0 and 30°C | Trypanosoma cruzi | |
0.073 | - |
pyridoxal | in the presence of K+, at pH 7.0 and 30°C | Plasmodium vivax | |
0.12 | - |
pyridoxal | in the presence of K+, at pH 7.0 and 30°C | Homo sapiens | |
0.23 | - |
pyridoxal | in the presence of Na+, at pH 7.0 and 30°C | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0572 | - |
primaquine | at pH 7.0 and 30°C | Homo sapiens |