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Literature summary for 2.7.1.35 extracted from

  • Kim, M.I.; Hong, M.
    Crystal structure and catalytic mechanism of pyridoxal kinase from Pseudomonas aeruginosa (2016), Biochem. Biophys. Res. Commun., 478, 300-306.
    View publication on PubMed
Search for more literature for 2.7.1.35

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 0.1 M Tris, pH 8.5, 1 M LiCl2, and 15% (w/v) PEG 6000 Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
C124A the mutant shows about 2fold increased catalytic efficiency compared to the wild type enzyme Pseudomonas aeruginosa
D225A inactive Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.037
-
 pyridoxal mutant enzyme C124A, at pH 7.4 and 37°C Pseudomonas aeruginosa
0.053
-
 pyridoxal wild type enzyme, at pH 7.4 and 37°C Pseudomonas aeruginosa
0.078
-
 ATP mutant enzyme C124A, at pH 7.4 and 37°C Pseudomonas aeruginosa
0.102
-
 ATP wild type enzyme, at pH 7.4 and 37°C Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + pyridoxal Pseudomonas aeruginosa
-
 ADP + pyridoxal 5'-phosphate
-
 ?
ATP + pyridoxal Pseudomonas aeruginosa PAO
-
 ADP + pyridoxal 5'-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-
Pseudomonas aeruginosa PAO
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Mono Q column chromatography Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pyridoxal
-
 Pseudomonas aeruginosa ADP + pyridoxal 5'-phosphate
-
 ?
ATP + pyridoxal
-
 Pseudomonas aeruginosa PAO ADP + pyridoxal 5'-phosphate
-
 ?

Subunits

Subunits Comment Organism
homodimer
-
 Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
PdxK
-
 Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3
-
 pyridoxal wild type enzyme, at pH 7.4 and 37°C Pseudomonas aeruginosa
1.8
-
 ATP mutant enzyme C124A, at pH 7.4 and 37°C Pseudomonas aeruginosa
3.3
-
 ATP wild type enzyme, at pH 7.4 and 37°C Pseudomonas aeruginosa
4.7
-
 pyridoxal mutant enzyme C124A, at pH 7.4 and 37°C Pseudomonas aeruginosa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
12.7
-
 ATP wild type enzyme, at pH 7.4 and 37°C Pseudomonas aeruginosa
23.1
-
 ATP mutant enzyme C124A, at pH 7.4 and 37°C Pseudomonas aeruginosa
62.3
-
 pyridoxal wild type enzyme, at pH 7.4 and 37°C Pseudomonas aeruginosa
127
-
 pyridoxal mutant enzyme C124A, at pH 7.4 and 37°C Pseudomonas aeruginosa