Application | Comment | Organism |
---|---|---|
medicine | ingestion of 4'-O-methylpyridoxine, i.e. ginkgotoxin, triggers epileptic convulsions and other neuronal symptoms. 4'-O-Methylpyridoxine is an competitive inhibitor of pyridoxal kinase and leads to temporarily reduced pyridoxal phosphate formation in vitro and possibly in vivo | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
expressed in Escherichia coli strain BL21 (DE3) | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4'-deoxypyridoxine | - |
Homo sapiens | |
4'-O-methylpyridoxine | i.e. ginkgotoxin. Treatment leads to temporarily reduced pyridoxal phosphate formation in vitro and possibly in vivo | Homo sapiens | |
ginkgotoxin | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
pyridoxal | in 70 mM potassium phosphate buffer, pH 6.2 | Homo sapiens | |
0.00495 | - |
4'-O-methylpyridoxine | pH 6.2, 37°C | Homo sapiens | |
0.00495 | - |
ginkgotoxin | in 70 mM potassium phosphate buffer, pH 6.2 | Homo sapiens | |
0.00987 | - |
pyridoxine | pH 6.2, 37°C | Homo sapiens | |
0.00987 | - |
pyridoxine | in 70 mM potassium phosphate buffer, pH 6.2 | Homo sapiens | |
0.0216 | - |
4'-deoxypyridoxine | pH 6.2, 37°C | Homo sapiens | |
0.0216 | - |
4'-deoxypyridoxine | in 70 mM potassium phosphate buffer, pH 6.2 | Homo sapiens | |
0.0587 | - |
pyridoxal | pH 6.2, 37°C | Homo sapiens | |
0.126 | - |
pyridoxamine | pH 6.2, 37°C | Homo sapiens | |
0.126 | - |
pyridoxamine | in 70 mM potassium phosphate buffer, pH 6.2 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O00764 | - |
- |
Purification (Comment) | Organism |
---|---|
pyridoxyl-EAH-Sepharose 4B column chromatography | Homo sapiens |
recombinant enzyme | Homo sapiens |
Storage Stability | Organism |
---|---|
-20°C, recombinant enzyme, stable for 21 days | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4'-deoxypyridoxine | - |
Homo sapiens | ADP + 4'-deoxypyridoxine 5'-phosphate | - |
? | |
ATP + 4'-O-methylpyridoxine | - |
Homo sapiens | ADP + 4'-O-methylpyridoxine 5'-phosphate | - |
? | |
ATP + ginkgotoxin | - |
Homo sapiens | ADP + ginkgotoxin 5'-phosphate | - |
? | |
ATP + pyridoxal | - |
Homo sapiens | ADP + pyridoxal 5'-phosphate | - |
? | |
ATP + pyridoxal | no substrate: GTP | Homo sapiens | ADP + pyridoxal 5'-phosphate | - |
? | |
ATP + pyridoxamine | - |
Homo sapiens | ADP + pyridoxamine 5'-phosphate | - |
? | |
ATP + pyridoxine | - |
Homo sapiens | ADP + pyridoxine 5'-phosphate | - |
? | |
additional information | no activity with GTP | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PKH | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
-20 | - |
stable at -20°C for approximately 21 days | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.46 | - |
ginkgotoxin | in 70 mM potassium phosphate buffer, pH 6.2 | Homo sapiens | |
0.46 | - |
4'-O-methylpyridoxine | pH 6.2, 37°C | Homo sapiens | |
0.79 | - |
pyridoxamine | in 70 mM potassium phosphate buffer, pH 6.2 | Homo sapiens | |
0.79 | - |
pyridoxamine | pH 6.2, 37°C | Homo sapiens | |
0.995 | - |
pyridoxal | pH 6.2, 37°C | Homo sapiens | |
1.233 | - |
pyridoxine | pH 6.2, 37°C | Homo sapiens | |
1.535 | - |
4'-deoxypyridoxine | pH 6.2, 37°C | Homo sapiens | |
2.94 | - |
4'-deoxypyridoxine | in 70 mM potassium phosphate buffer, pH 6.2 | Homo sapiens | |
6.08 | - |
pyridoxal | in 70 mM potassium phosphate buffer, pH 6.2 | Homo sapiens | |
6.08 | - |
pyridoxine | in 70 mM potassium phosphate buffer, pH 6.2 | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | 6.3 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000414 | - |
ginkgotoxin | - |
Homo sapiens | |
0.000414 | - |
4'-O-methylpyridoxine | pH 6.2, 37°C | Homo sapiens | |
0.0574 | - |
4'-deoxypyridoxine | - |
Homo sapiens | |
0.0574 | - |
4'-deoxypyridoxine | pH 6.2, 37°C | Homo sapiens |