Cloned (Comment) | Organism |
---|---|
expressed as His-tag fusion protein in Escherichia coli BL21(DE3) | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.047 | - |
pyridoxal | pH 8, 37°C | Bacillus subtilis | |
2.03 | - |
4-Amino-5-hydroxymethyl-2-methylpyrimidine | pH 8, 37°C | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyridoxal | Bacillus subtilis | generates the active form of vitamin B6, which serves as cofactor for many enzymes | ADP + pyridoxal 5'-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein using His-tag | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine | - |
Bacillus subtilis | ADP + 4-amino-5-phosphomethyl-2-methylpyrimidine | - |
? | |
ATP + pyridoxal | - |
Bacillus subtilis | ADP + pyridoxal 5'-phosphate | - |
? | |
ATP + pyridoxal | generates the active form of vitamin B6, which serves as cofactor for many enzymes | Bacillus subtilis | ADP + pyridoxal 5'-phosphate | - |
? | |
ATP + pyridoxamine | - |
Bacillus subtilis | ADP + pyridoxamine 5'-phosphate | - |
? | |
ATP + pyridoxine | - |
Bacillus subtilis | ADP + pyridoxine 5'-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PdxK | formerly ThiD | Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
pyridoxal | pH 8, 37°C | Bacillus subtilis | |
0.36 | - |
4-Amino-5-hydroxymethyl-2-methylpyrimidine | pH 8, 37°C | Bacillus subtilis |