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Literature summary for 2.7.1.35 extracted from

  • Lee, H.S.; Moon, B.J.; Choi, S.Y.; Kwon, O.S.
    Human pyridoxal kinase: overexpression and properties of the recombinant enzyme (2000), Mol. Cells, 10, 452-459.
    View publication on PubMed
Search for more literature for 2.7.1.35

Cloned(Commentary)

Cloned (Comment) Organism
recombinant enzyme is overexpressed in Escherichia coli as a fusion protein with maltose binding protein Homo sapiens

General Stability

General Stability Organism
3 M guanidine hydrochloride, enzyme loses more than 90% of the alpha-helix content Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Cr2+
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 ATP pH 7, 37°C Homo sapiens
0.097
-
 pyridoxal pH 7, 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ divalent cation required, activation of the recombinant enzyme in the order of decreasing efficiency: Zn2+, Co2+, Mn2+, Mg2+, Ca2+ Homo sapiens
Co2+ divalent cation required, activation of the recombinant enzyme in the order of decreasing efficiency: Zn2+, Co2+, Mn2+, Mg2+, Ca2+ Homo sapiens
Mg2+ divalent cation required, activation of the recombinant enzyme in the order of decreasing efficiency: Zn2+, Co2+, Mn2+, Mg2+, Ca2+ Homo sapiens
Mn2+ divalent cation required, activation of the recombinant enzyme in the order of decreasing efficiency: Zn2+, Co2+, Mn2+, Mg2+, Ca2+ Homo sapiens
Zn2+ divalent cation required, activation of the recombinant enzyme in the order of decreasing efficiency: Zn2+, Co2+, Mn2+, Mg2+, Ca2+. Optimum at about 0.1 mM Zn2+ Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Homo sapiens

Renatured (Commentary)

Renatured (Comment) Organism
the enzyme which has lost more than 90% of the alpha-helix content after treatment with 3 M guanidine hydrochloride regains more than 90% of the original catalytic activity after overnight dialysis against 10 mM potassium phosphate, pH 7 at 4°C Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.47
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pyridoxal
-
 Homo sapiens ADP + pyridoxal 5'-phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 40000, SDS-PAGE Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 pH 5.5-11, 3 h, stable Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
5 8 pH 5.0: about 70% of maximal activity, pH 8.0: about 50% of maximal activity Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 11 25°C, 3 h, stable. Gradual irreversible inactivation below pH 5.5 Homo sapiens