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Literature summary for 2.7.1.35 extracted from
- Physical and kinetic properties of sheep liver pyridoxine kinase (1982), Arch. Biochem. Biophys., 216, 170-177.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| NH4+ | Km: 3.7 mM. Monovalent cation required, activation in the order of decreasing efficiency: K+, Rb+, NH4+ | Ovis aries |  |
General Stability
| General Stability | Organism |
|---|---|
| impure enzyme is stabilized by Zn2+ | Ovis aries |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | excess free divalent cations inhibit the enzyme | Ovis aries | |
| Mn2+ | excess free divalent cations inhibit the enzyme | Ovis aries | |
| Zn2+ | excess free divalent cations inhibit the enzyme | Ovis aries | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | effect of KCl on Km-values | Ovis aries | |
| 0.06 | - |
pyridoxine | pH 5.8, 37°C, with MnATP2- as the second substrate | Ovis aries | |
| 0.06 | - |
ATP | pH 5.8, 37°C | Ovis aries | |
| 0.06 | - |
ATP | ATP in form of ZnATP2- | Ovis aries | |
| 0.101 | - |
ATP | pH 5.8, 37°C | Ovis aries | |
| 0.101 | - |
ATP | ATP in form of MgATP2- | Ovis aries | |
| 0.194 | - |
ATP | pH 5.8, 37°C | Ovis aries | |
| 0.194 | - |
ATP | ATP in form of MnATP2- | Ovis aries | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | Km: 8.9 mM. Monovalent cation required, activation in the order of decreasing efficiency: K+, Rb+, NH4+ | Ovis aries | |
| Rb+ | Km: 5.3 mM. Monovalent cation required, activation in the order of decreasing efficiency: K+, Rb+, NH4+ | Ovis aries | |
| Zn2+ | the enzyme requires divalent cations for activity. At 0.08 mM the cations activate in order of decreasing efficiency: Mn2+, Zn2+, Mg2+. At 0.4 mM the cations activate in the order of decreasing efficiency: Mn2+, Zn2+, Mg2+ | Ovis aries | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
gel filtration, equilibrium sedimentation | Ovis aries |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ovis aries | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ovis aries |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Ovis aries | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + pyridoxal | - |
Ovis aries | ADP + pyridoxal 5'-phosphate | - |
? | |
| ATP + pyridoxine | - |
Ovis aries | ADP + pyridoxine 5'-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 60000, SDS-PAGE | Ovis aries |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6 | - |
Ovis aries |
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Release 2023.1 (January 2023)
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