Literature summary for 2.7.1.33 extracted from

Satoh, S.; Ozaki, M.; Matsumoto, S.; Nabatame, T.; Kaku, M.; Shudo, T.; Asayama, M.; Chohnan, S.
Enhancement of fatty acid biosynthesis by exogenous acetyl-CoA carboxylase and pantothenate kinase in Escherichia coli (2020), Biotechnol. Lett., 42, 2595-2605 .

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Cloned(Commentary)

Cloned (Comment)	Organism
gene coaX, functional recombinant expression in Escherichia coli	Pseudomonas putida

Protein Variants

Protein Variants	Comment	Organism
additional information	expression of acetyl-CoA carboxylase (acc) obtained from Corynebacterium glutamicum in Escherichia coli, it causes accumulation of 2.2fold more fatty acids in Escherichia coli that in the wild-type. The addition of gene coaX encoding patothenate kinase from Pseudomonas putida or fatty acid synthase (fasA) from Corynebacterium glutamicum results in a 3.1 and 3.6fold increased fatty acid synthesis in Escherichia coli cells, which express acc and coaA, or acc and fasA, respectively. The transformants, simultaneously possessing all three genes, produce 5.6fold more fatty acids. The strain possessing acc, coaA, and fasA stores 691 mg/l of fatty acids, primarily as phospholipids, inside the inner membrane after 72-h cultivation. In addition, 19% of the total CoA pool is occupied by malonyl-CoA	Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + (R)-pantothenate	Pseudomonas putida	-	ADP + (R)-4'-phosphopantothenate	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	V5XWU6	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + (R)-pantothenate	-	Pseudomonas putida	ADP + (R)-4'-phosphopantothenate	-	?

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Release 2023.1 (January 2023)