Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no feedback regulation by CoA or acetyl-CoA | Staphylococcus aureus |
Cloned (Comment) | Organism |
---|---|
gene coaA, expression of His6-tagged in Escherichia coli strain BL21(DE3) | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no feedback regulation by CoA or acetyl-CoA | Staphylococcus aureus | |
N-heptylpantothenamide | IC50 is 0.0048 mM, potent growth inhibitory anti-metabolite | Staphylococcus aureus | |
N-pentylpantothenamide | IC50 is 0.0035 mM, has antimicrobial activity against Staphylococcus aureus | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
N-pentylpantothenamide | pH 7.5, 37°C, recombinant enzyme | Staphylococcus aureus | |
0.008 | - |
N-heptylpantothenamide | pH 7.5, 37°C, recombinant enzyme | Staphylococcus aureus | |
0.023 | - |
(R)-pantothenate | pH 7.5, 37°C, recombinant enzyme | Staphylococcus aureus | |
0.034 | - |
ATP | pH 7.5, 37°C, recombinant enzyme | Staphylococcus aureus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Staphylococcus aureus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
2 * 29000, recombinant enzyme, SDS-PAGE | Staphylococcus aureus |
59000 | - |
recombinant enzyme, gel filtration | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-pantothenate | Staphylococcus aureus | first step in coenzyme A biosynthesis | ADP + (R)-4'-phosphopantothenate | - |
? | |
ATP + (R)-pantothenate | Staphylococcus aureus RN4220 | first step in coenzyme A biosynthesis | ADP + (R)-4'-phosphopantothenate | - |
? | |
ATP + N-heptylpantothenamide | Staphylococcus aureus | substrate is converted to the inactive butyldethia-CoA analogue in the further downstream pathway leading to grwoth inhibition of the organism, overview | ADP + N-heptylpantothenamide 4-phosphate | - |
? | |
ATP + N-heptylpantothenamide | Staphylococcus aureus RN4220 | substrate is converted to the inactive butyldethia-CoA analogue in the further downstream pathway leading to grwoth inhibition of the organism, overview | ADP + N-heptylpantothenamide 4-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
gene coaA | - |
Staphylococcus aureus RN4220 | - |
gene coaA | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Staphylococcus aureus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-pantothenate | - |
Staphylococcus aureus | ADP + (R)-4'-phosphopantothenate | - |
? | |
ATP + (R)-pantothenate | first step in coenzyme A biosynthesis | Staphylococcus aureus | ADP + (R)-4'-phosphopantothenate | - |
? | |
ATP + (R)-pantothenate | - |
Staphylococcus aureus RN4220 | ADP + (R)-4'-phosphopantothenate | - |
? | |
ATP + (R)-pantothenate | first step in coenzyme A biosynthesis | Staphylococcus aureus RN4220 | ADP + (R)-4'-phosphopantothenate | - |
? | |
ATP + N-heptylpantothenamide | - |
Staphylococcus aureus | ADP + N-heptylpantothenamide 4-phosphate | - |
? | |
ATP + N-heptylpantothenamide | substrate is converted to the inactive butyldethia-CoA analogue in the further downstream pathway leading to grwoth inhibition of the organism, overview | Staphylococcus aureus | ADP + N-heptylpantothenamide 4-phosphate | - |
? | |
ATP + N-heptylpantothenamide | - |
Staphylococcus aureus RN4220 | ADP + N-heptylpantothenamide 4-phosphate | - |
? | |
ATP + N-heptylpantothenamide | substrate is converted to the inactive butyldethia-CoA analogue in the further downstream pathway leading to grwoth inhibition of the organism, overview | Staphylococcus aureus RN4220 | ADP + N-heptylpantothenamide 4-phosphate | - |
? | |
ATP + N-pentylpantothenamide | - |
Staphylococcus aureus | ADP + N-pentylpantothenamide 4-phosphate | - |
? | |
ATP + N-pentylpantothenamide | - |
Staphylococcus aureus RN4220 | ADP + N-pentylpantothenamide 4-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 29000, recombinant enzyme, SDS-PAGE | Staphylococcus aureus |
Synonyms | Comment | Organism |
---|---|---|
CoaA | - |
Staphylococcus aureus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Staphylococcus aureus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
N-pentylpantothenamide | pH 7.5, 37°C, recombinant enzyme | Staphylococcus aureus | |
0.425 | - |
N-heptylpantothenamide | pH 7.5, 37°C, recombinant enzyme | Staphylococcus aureus | |
1.6 | - |
ATP | pH 7.5, 37°C, recombinant enzyme | Staphylococcus aureus | |
1.7 | - |
(R)-pantothenate | pH 7.5, 37°C, recombinant enzyme | Staphylococcus aureus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Staphylococcus aureus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Staphylococcus aureus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0035 | - |
IC50 is 0.0035 mM, has antimicrobial activity against Staphylococcus aureus | Staphylococcus aureus | N-pentylpantothenamide | |
0.0048 | - |
IC50 is 0.0048 mM, potent growth inhibitory anti-metabolite | Staphylococcus aureus | N-heptylpantothenamide |