Cloned (Comment) | Organism |
---|---|
gene licA, recombinant expression of enzyme sChoK in Escherichia coli strain BL21(DE3) | Streptococcus pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,1'-[butane-1,4-diylbis[(4,1-phenylene)methylene]]bis[4-(dimethylamino)pyridin-1-ium] | MN58b, mixed inhibition type with both choline and ATP | Streptococcus pneumoniae | |
1,1'-[[1,1'-biphenyl]-4,4'-diylbis(methylene)]bis[4-[(4-chlorophenyl)(methyl)amino]quinolin-1-ium] | RSM-932A, mixed inhibition type with choline, non-competitive with ATP | Streptococcus pneumoniae | |
hemicholinium-3 | HC-3, MIC is 5.4 mM | Streptococcus pneumoniae | |
additional information | docking of MN58b and RSM-932A onto sChoK, overview. MN58b and RSM-932A fit into the active site of sChoK in a differential manner. In enzymatic assays with Streptoccocus pneumoniae extracts MN58b is still an inhibitor, while RSM-932A becomes an agonist | Streptococcus pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics | Streptococcus pneumoniae | |
0.13 | - |
choline | recombinant enzyme, pH 8.0, 25°C | Streptococcus pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Streptococcus pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + choline | Streptococcus pneumoniae | - |
ADP + phosphocholine | - |
? | |
ATP + choline | Streptococcus pneumoniae ATCC BAA-255 | - |
ADP + phosphocholine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | Q8DPI4 | - |
- |
Streptococcus pneumoniae ATCC BAA-255 | Q8DPI4 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme sChoK from Escherichia coli strain BL21(DE3) | Streptococcus pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + choline | - |
Streptococcus pneumoniae | ADP + phosphocholine | - |
? | |
ATP + choline | - |
Streptococcus pneumoniae ATCC BAA-255 | ADP + phosphocholine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
chbA | - |
Streptococcus pneumoniae |
ChoK | - |
Streptococcus pneumoniae |
LicA | - |
Streptococcus pneumoniae |
sChoK | - |
Streptococcus pneumoniae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Streptococcus pneumoniae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.14 | - |
choline | recombinant enzyme, pH 8.0, 25°C | Streptococcus pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Streptococcus pneumoniae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics and MIC values | Streptococcus pneumoniae | |
0.0106 | - |
1,1'-[[1,1'-biphenyl]-4,4'-diylbis(methylene)]bis[4-[(4-chlorophenyl)(methyl)amino]quinolin-1-ium] | recombinant enzyme, pH 8.0, 25°C, versus choline | Streptococcus pneumoniae | |
0.0358 | - |
1,1'-[[1,1'-biphenyl]-4,4'-diylbis(methylene)]bis[4-[(4-chlorophenyl)(methyl)amino]quinolin-1-ium] | recombinant enzyme, pH 8.0, 25°C, versus ATP | Streptococcus pneumoniae | |
0.124 | - |
1,1'-[butane-1,4-diylbis[(4,1-phenylene)methylene]]bis[4-(dimethylamino)pyridin-1-ium] | recombinant enzyme, pH 8.0, 25°C, versus choline | Streptococcus pneumoniae | |
0.182 | - |
1,1'-[butane-1,4-diylbis[(4,1-phenylene)methylene]]bis[4-(dimethylamino)pyridin-1-ium] | recombinant enzyme, pH 8.0, 25°C, versus ATP | Streptococcus pneumoniae |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
2.7 | - |
recombinant enzyme, pH 8.0, 25°C | Streptococcus pneumoniae | hemicholinium-3 |
General Information | Comment | Organism |
---|---|---|
malfunction | choline kinase inhibitor MN58b sensitizes Streptococcus pneumoniae cells to autolysis while inhibitor RSM-932A does not. MN58b and RSM-932A do not provoke or prevent autolysis. Effect of the drugs on LTA production, overview. MN58b but not RSM-932A causes deformation of the Streptococcus pneumoniae cell wall | Streptococcus pneumoniae |
metabolism | the sChoK enzyme is an element of the pathway that mediates the decoration of teichoic acids with PCho via the intermediary CDP-choline | Streptococcus pneumoniae |
physiological function | the choline kinase of S. pneumoniae (sChoK) is an essential enzyme. Choline kinase (ChoK) phosphorylates choline into phosphocholine. Phosphocholine is important because it a precursor molecule that is utilized in the production of two types of teichoic acids, lipoteichoic acid (LTA) and cell wall teichoic acid (CTA). sChoK plays a role in the growth and pathogenesis for humans of Gram-positive bacterium Streptococcus pneumoniae. In Streptococcus pneumoniae, both acids consist of the same types of polysaccharides but LTA is attached to a lipid and embedded in the cell membrane, and CTA is attached to the peptidoglycan molecules in the cell wall. LTA is an important virulence factor and LTA production has been validated as a drug target | Streptococcus pneumoniae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
24.15 | - |
choline | recombinant enzyme, pH 8.0, 25°C | Streptococcus pneumoniae |