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Literature summary for 2.7.1.32 extracted from
- Arabidopsis CHOLINE/ETHANOLAMINE KINASE 1 (CEK1) is a primary choline kinase localized at the endoplasmic reticulum (ER) and involved in ER stress tolerance (2019), New Phytol., 223, 1904-1917 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene CK1, recombinant overexpression in Arabidopsis thaliana plants via Agrobacterium tumefaciens-mediated transformation, quantitative RT-PCR enzyme expression analysis at normal or tunicamycin (Tm)-induced endoplasmic reticulum (ER) stress conditions | Arabidopsis thaliana |
| locus At1g74320, recombinant overexpression in Arabidopsis thaliana plants via Agrobacterium tumefaciens-mediated transformation, quantitative RT-PCR enzyme expression analysis at normal or tunicamycin (Tm)-induced endoplasmic reticulum (ER) stress conditions | Arabidopsis thaliana |
| locus At4g09760, recombinant overexpression in Arabidopsis thaliana plants via Agrobacterium tumefaciens-mediated transformation, quantitative RT-PCR enzyme expression analysis at normal or tunicamycin (Tm)-induced endoplasmic reticulum (ER) stress conditions | Arabidopsis thaliana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| moe | construction of isozyme mutants cek1-1, cek2-1, and cek3-1, and seedling phenotype of the Arabidopsis thaliana cek1-1, cek2-1, and cek3-1 mutants in response to tunicamycin (Tm)-induced endoplasmic reticulum (ER) stress, overview | Arabidopsis thaliana |
| moe | construction of isozyme mutants cek1-1, cek2-1, and cek3-1, and seedling phenotype of the Arabidopsis thaliana cek1-1, cek2-1, and cek3-1 mutants in response to tunicamycin (Tm)-induced endoplasmic reticulum (ER) stress,overview | Arabidopsis thaliana |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | - |
Arabidopsis thaliana | 5783 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Arabidopsis thaliana |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + choline | Arabidopsis thaliana | - |
ADP + phosphocholine | - |
? | |
| ATP + choline | Arabidopsis thaliana Col-0 | - |
ADP + phosphocholine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q8L518 | - |
- |
| Arabidopsis thaliana | Q9M9H6 | - |
- |
| Arabidopsis thaliana | Q9SZ92 | - |
- |
| Arabidopsis thaliana Col-0 | Q8L518 | - |
- |
| Arabidopsis thaliana Col-0 | Q9M9H6 | - |
- |
| Arabidopsis thaliana Col-0 | Q9SZ92 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | CEK1 is preferentially expressed in vegetative tissue, with roots as the primary tissue for the expression | Arabidopsis thaliana | - |
| root | CEK1 is highly expressed in the root tips of germinating seedlings at days 1-4 | Arabidopsis thaliana | - |
| seedling | - |
Arabidopsis thaliana | - |
| seedling | CEK1 is highly expressed in the root tips of germinating seedlings at days 1-4 | Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + choline | - |
Arabidopsis thaliana | ADP + phosphocholine | - |
? | |
| ATP + choline | - |
Arabidopsis thaliana Col-0 | ADP + phosphocholine | - |
? | |
| additional information | detection via quantitative TLC | Arabidopsis thaliana | ? | - |
- |
|
| additional information | detection via quantitative TLC | Arabidopsis thaliana Col-0 | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CEK1 | - |
Arabidopsis thaliana |
| CEK2 | - |
Arabidopsis thaliana |
| CEK3 | - |
Arabidopsis thaliana |
| choline/ethanolamine kinase 1 | - |
Arabidopsis thaliana |
| choline/ethanolamine kinase 2 | - |
Arabidopsis thaliana |
| choline/ethanolamine kinase 3 | - |
Arabidopsis thaliana |
| CK1 | - |
Arabidopsis thaliana |
| CK2 | - |
Arabidopsis thaliana |
| CK3 | - |
Arabidopsis thaliana |
| ER-localized Cho kinase | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Arabidopsis thaliana |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | in Arabidopsis thaliana, the CEK family consists of four isoforms. Isozymes in this family may phosphorylate choline (Cho kinase, EC 2.7.1.32) or ethanolamine (Etn kinase, EC 2.7.1.82) in the presence of ATP and magnesium ions, producing phosphocholine (PCho) or phosphoethanolamine (PEtn), respectively | Arabidopsis thaliana |
| malfunction | under tunicamycin (Tm) treatment, seedling growth is retarded in mutants cek2-1, cek3-1 and wild-type, mutant cek1-1 exhibits severe growth defects. The expression of the other CEK isoforms (CEK2, CEK3, and CEK4) does not change significantly in either wild-type or cek1-1 before and after Tm treatment | Arabidopsis thaliana |
| malfunction | upon tunicamycin-induced ER stress, a null mutant of CEK1 shows hypersensitive phenotype in seedlings, albeit with no enhanced choline kinase activity. Analysis of the hypersensitive phenotype of a cek1 mutant under tunicamycin-induced endoplasmic reticulum stress. Under tunicamycin (Tm) treatment, seedling growth is retarded in mutants cek2-1, cek3-1 and wild-type, mutant cek1-1 exhibits severe growth defects. The expression of the other CEK isoforms (CEK2, CEK3, and CEK4) does not change significantly in either wild-type or cek1-1 before and after Tm treatment | Arabidopsis thaliana |
| additional information | analysis of Cho-containing compounds in seedlings by ESI-LC-MS | Arabidopsis thaliana |
| additional information | analysis of choline-containing compounds in seedlings by ESI-LC-MS | Arabidopsis thaliana |
| physiological function | choline kinase catalyzes the initial reaction step of choline metabolism that produces phosphocholine, a prerequisite for the biosynthesis of a primary phospholipid phosphatidylcholine | Arabidopsis thaliana |
| physiological function | choline kinase catalyzes the initial reaction step of choline metabolism that produces phosphocholine, a prerequisite for the biosynthesis of a primary phospholipid phosphatidylcholine. Arabidopsis thaliana choline/ethanolamine kinase 1 (CEK1) encodes functional CEK that prefers choline than ethanolamine as a substrate in vitro and affects contents of choline and phosphocholine but not phosphatidylcholine in vivo. CEK1 is a primary endoplasmic reticulum-localized choline kinase in vivo that is required for ER stress tolerance possibly through the modulation of choline metabolites | Arabidopsis thaliana |
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