Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta cells and in Saccharomyces cerevisiae KS106 cells | Toxoplasma gondii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dimethylethanolamine | - |
Toxoplasma gondii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.77 | - |
choline | in 100 mM Tris-HCl (pH 8.5), at 37°C | Toxoplasma gondii | |
0.94 | - |
dimethylethanolamine | in 100 mM Tris-HCl (pH 8.5), at 37°C | Toxoplasma gondii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Toxoplasma gondii | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
x * 44000, the parasite has two potential 53000 and 44000 Da isoforms, SDS-PAGE | Toxoplasma gondii |
53000 | - |
x * 53000, the parasite has two potential 53000 and 44000 Da isoforms, SDS-PAGE | Toxoplasma gondii |
70000 | - |
x * 70000, full-length enzyme, SDS-PAGE | Toxoplasma gondii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Toxoplasma gondii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Toxoplasma gondii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
tachyzoite | - |
Toxoplasma gondii | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + choline | - |
Toxoplasma gondii | ADP + phosphocholine | - |
? | |
ATP + dimethylethanolamine | - |
Toxoplasma gondii | ADP + phosphodimethylethanolamine | - |
? | |
ATP + ethanolamine | - |
Toxoplasma gondii | ADP + phosphoethanolamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 44000, the parasite has two potential 53000 and 44000 Da isoforms, SDS-PAGE | Toxoplasma gondii |
? | x * 53000, the parasite has two potential 53000 and 44000 Da isoforms, SDS-PAGE | Toxoplasma gondii |
? | x * 70000, full-length enzyme, SDS-PAGE | Toxoplasma gondii |
General Information | Comment | Organism |
---|---|---|
malfunction | the parasite compensates for the loss of full-length enzyme by two potential 53- and 44 kDa isoforms | Toxoplasma gondii |