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Literature summary for 2.7.1.32 extracted from

  • Hudson, C.S.; Knegtel, R.M.; Brown, K.; Charlton, P.A.; Pollard, J.R.
    Kinetic and mechanistic characterisation of choline kinase-alpha (2013), Biochim. Biophys. Acta, 1834, 1107-1116.
    View publication on PubMed
Search for more literature for 2.7.1.32

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with inhibitor V-11-023907, hanging drop vapor diffusion method, using 0.1 M Bis-Tris (pH 6.5), 25% (w/v) polyethylene glycol 3350, and 0.1 M MgCl2 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
V-11-023907
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.188
-
 choline in 100 mM Tris-HCl pH 7.5, 100 mM KCl, 10 mM MgCl2, at 25°C Homo sapiens
0.486
-
 ATP in 100 mM Tris-HCl pH 7.5, 100 mM KCl, 10 mM MgCl2, at 25°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-

Organism

Organism UniProt Comment Textmining
Homo sapiens P35790
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione affinity column chromatography, Ni-NTA column chromatography, and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + choline
-
 Homo sapiens ADP + phosphocholine
-
 ?

Synonyms

Synonyms Comment Organism
ChoKalpha
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
71
-
 choline in 100 mM Tris-HCl pH 7.5, 100 mM KCl, 10 mM MgCl2, at 25°C Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00047
-
 in 100 mM Tris-HCl pH 7.5, 100 mM KCl, 10 mM MgCl2, at 25°C Homo sapiens V-11-023907