Cloned (Comment) | Organism |
---|---|
A mouse embryonic stem cell line (XH252, strain 129/OlaHsd) with an insertional mutation in choline kinase alpha is created in a gene-trapping prgram. The vector, pGT1Lxf, is designed to create an in-frame fusion between the 5' exons of the trapped gene and a reporter, betageo (a fusion of beta-galactosidase and neomycin phosphotransferase 2). CK-alpha spans 12 exons on mouse chromosome 19. The insertional mutation in XH252 occurred in intron 5. Thus, the gene-trapped locus is predicted to yield a fusion transcript containing exons 1-5 of CK-alpha and betageo. The embryonic stem cells are injected into C57BL/6 blastocysts to create chimeric mice, which are bred with C57BL/6 mice to generate heterozygous (+/-) CK-alpha-deficient mice. All mice had a mixed genetic background. The mice are weaned at 21 days of age, housed in a barrier facility with a 12-h light-dark cycle, and fed chow containing 4.5% fat. Creating of mice lacking CK- alpha with an embryonic stem cell line containing an insertional mutation in the gene for CK-alpha. Embryos homozygous for the mutant CK-alpha allele are recovered at the blastocyst stage, but not at embryonic day 7.5, indicating that CK-alpha is crucial for the early development of mouse embryos. | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the lethality of the homozygous choline kinase alpha knock-out mice embryos indicates the indispensable role of CK-alphain early embryogenesis | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | assay at | Mus musculus | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
choline + ATP | Mus musculus | catalyzes the first phosphorylation reaction in the Kennedy pathway (biosynthesis of the major membrane phospholipid phosphatidylcholine) | phosphocholine + ADP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
knock-out mice, heterozygous, +/-choline kinase alpha deficient | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Mus musculus | - |
skeletal muscle | forelimb, hindlimb | Mus musculus | - |
testis | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000139 | - |
skeletal muscle, hindlimbs of +/+ CK-alpha mice, supernatant of tissue homogenates are incubated in volume of 0.100 ml of reaction buffer at 37°C for 30 min. The reaction product, phosphocholine, is separated using an AG1-X8 column.To determine the activity of each CK isoform, supernatant fractions are treated with an antisera raised against GST (control), GST-CK-alpha or GST-CK-beta fusion proteins combined with protein A-Sepharose overnight at 4°C, and the supernatant is used. | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
choline + ATP | catalyzes the first phosphorylation reaction in the Kennedy pathway (biosynthesis of the major membrane phospholipid phosphatidylcholine) | Mus musculus | phosphocholine + ADP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
choline kinase alpha | - |
Mus musculus |
CK | - |
Mus musculus |
CK-alpha | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
assay at | Mus musculus |