Literature summary for 2.7.1.32 extracted from

Ramirez de Molina, A.; Gallego-Ortega, D.; Sarmentero-Estrada, J.; Lagares, D.; Gomez Del Pulgar, T.; Bandres, E.; Garcia-Foncillas, J.; Lacal, J.C.
Choline kinase as a link connecting phospholipid metabolism and cell cycle regulation: implications in cancer therapy (2008), Int. J. Biochem. Cell Biol., 40, 1753-1763.

Application

Application	Comment	Organism
diagnostics	prognostic factor in human cancer	Homo sapiens
drug development	target for therapy since its specific inhibitors display efficient antitumoral activity in vivo	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
Transfection of human HeK293T is carried out by the calcium phosphate method. The amount of plasmidic DNA is kept constant at 0.2 mg with the corresponding empty vector. Tumors induced by ChoK alpha over-expression in Hek293T are removed from the mice to generate the ChoK tumoral cell line ADJ. Different slices of the tissue are placed into culture 100mm dishes and treated with trypsin and G418 to avoid murine cells contamination. The resultant cell line (ADJ) displays constitutive ChoK alpha activation and a fully transformed phenotype	Homo sapiens

Cloned (Comment)

Organism

Transfection of human HeK293T is carried out by the calcium phosphate method. The amount of plasmidic DNA is kept constant at 0.2 mg with the corresponding empty vector. Tumors induced by ChoK alpha over-expression in Hek293T are removed from the mice to generate the ChoK tumoral cell line ADJ. Different slices of the tissue are placed into culture 100mm dishes and treated with trypsin and G418 to avoid murine cells contamination. The resultant cell line (ADJ) displays constitutive ChoK alpha activation and a fully transformed phenotype

Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
HEK-293T cell	Transfection of human HeK293T is carried out by the calcium phosphate method. The amount of plasmidic DNA is kept constant at 2 microg with the corresponding empty vector. Tumors induced by ChoK alpha over-expression in Hek293T are removed from the mice to generate the ChoK tumoral cell line ADJ. Different slices of the tissue are placed into culture 100mm dishes and treated with trypsin and 1 mg/ml of G418 to avoid murine cells contamination. The resultant cell line ADJ displays constitutive ChoK alpha activation and a fully transformed phenotype	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	Genes significantly down-regulated by choline kinase: BCL2-interacting killer (apoptosis-inducing), stabilization of the calcium channel, cyclin G2. Inhibition of cell proliferation, T-cell surface protein tactile, cyclin-dependent kinase inhibitor 1A (p21, Cip1), cyclin-dependent kinase inhibitor 1B (p27, Kip1), dual-specificity-(Y)-tyrosine phosphor regulated kinase, Mad signalling (altered response to quimiotherapy), opioid receptor, mu 1 (negative regulat of cell proliferation), retinoblastoma-like 1 (p107) (Rb associated protein), transforming growth factor, beta receptor I, Toll like receptor (antitumoral immunity), immune escape of human cancer, Visinin like 1	Homo sapiens

Synonyms

Synonyms	Comment	Organism
ChoK	-	Homo sapiens
ChoK alpha	-	Homo sapiens
choline kinase	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Homo sapiens