Literature summary for 2.7.1.32 extracted from

Milanese, L.; Espinosa, A.; Campos, J.M.; Gallo, M.A.; Entrena, A.
Insight into the inhibition of human choline kinase: homology modeling and molecular dynamics simulations (2006), ChemMedChem, 1, 1216-1228.

Protein Variants

Protein Variants	Comment	Organism
D255A	omplete loss of activity	Caenorhabditis elegans
D255E	small residual activity	Caenorhabditis elegans
D255N	small residual activity	Caenorhabditis elegans
D301A	complete loss of activity	Caenorhabditis elegans
D301E	complete loss of activity	Caenorhabditis elegans
D301N	complete loss of activity	Caenorhabditis elegans
E125A	modest reduction of kcat and Km for ATP	Caenorhabditis elegans
E125D	no influence	Caenorhabditis elegans
E125Q	modest reduction of kcat and Km for ATP	Caenorhabditis elegans
E303A	strong reduction of the catalytic efficiency	Caenorhabditis elegans
E303D	small reduction of kcat	Caenorhabditis elegans
E303N	small reduction of kcat	Caenorhabditis elegans
E320A	reduction of ATP affinity and time instability	Caenorhabditis elegans
H253A	similar initial activity like wild type enzyme, but time instability	Caenorhabditis elegans
N254A	similar initial activity like wild type enzyme, but time instability	Caenorhabditis elegans
N260A	small residual activity	Caenorhabditis elegans
N308 A	small reduction of kcat	Caenorhabditis elegans
N308D	modest reduction of kcat	Caenorhabditis elegans
N308Q	modest reduction of kcat	Caenorhabditis elegans
N87A	small reduction of the catalytic efficiency	Caenorhabditis elegans
R111A	4-fold increase in Km for ATP	Caenorhabditis elegans
R149A	no influence	Caenorhabditis elegans
S86A	pronounced reduction of the catalytic efficiency	Caenorhabditis elegans
S86T	moderate reduction of the catalytic efficiency	Caenorhabditis elegans
W387A	strong reduction of kcat (30-fold)	Caenorhabditis elegans

Inhibitors

Inhibitors	Comment	Organism
Ca2+	-	Caenorhabditis elegans
Ca2+	-	Homo sapiens
hemicholinium-3	-	Caenorhabditis elegans
hemicholinium-3	-	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Caenorhabditis elegans	-	-	-
Homo sapiens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + choline	-	Homo sapiens	ADP + O-phosphocholine	-	?
ATP + choline	-	Caenorhabditis elegans	ADP + O-phosphocholine	-	?

Synonyms

Synonyms	Comment	Organism
CK-alpha	-	Homo sapiens
CKA-2	-	Caenorhabditis elegans

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Homo sapiens
ATP	-	Caenorhabditis elegans