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Literature summary for 2.7.1.32 extracted from

  • Choubey, V.; Guha, M.; Maity, P.; Kumar, S.; Raghunandan, R.; Maulik, P.R.; Mitra, K.; Halder, U.C.; Bandyopadhyay, U.
    Molecular characterization and localization of Plasmodium falciparum choline kinase (2006), Biochim. Biophys. Acta, 1760, 1027-1038.
    View publication on PubMed
Search for more literature for 2.7.1.32

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
ethanolamine poor inhibitor Plasmodium falciparum
phosphocholine 40% and 66% inhibition at 2.5 mM and 10 mM, respectively Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.145
-
 choline in 100 mM Tris/HCl, 6 mM MgCl2, 5 mM ATP, pH 8.8, at 37°C Plasmodium falciparum
2.5
-
 ATP in 100 mM Tris/HCl, 6 mM MgCl2, 5 mM ATP, pH 8.8, at 37°C Plasmodium falciparum
5
-
 ethanolamine in 100 mM Tris/HCl, 6 mM MgCl2, 5 mM ATP, pH 8.8, at 37°C Plasmodium falciparum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Plasmodium falciparum 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52200
-
 calculated from amino acid sequence Plasmodium falciparum
56000
-
 SDS-PAGE, recombinant protein Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
 clone NF54
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose resin column chromatography and 300SW gel filtration Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + choline
-
 Plasmodium falciparum ADP + O-phosphocholine
-
 ?
ATP + ethanolamine can not phosphorylate ethanolamine efficiently Plasmodium falciparum ADP + ethanolamine phosphate
-
 ?

Synonyms

Synonyms Comment Organism
PfCK
-
 Plasmodium falciparum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
-
 Plasmodium falciparum

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Plasmodium falciparum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.45
-
 phosphocholine in 100 mM Tris/HCl, 6 mM MgCl2, 5 mM ATP, pH 8.8, at 37°C Plasmodium falciparum
5.5
-
 ethanolamine in 100 mM Tris/HCl, 6 mM MgCl2, 5 mM ATP, pH 8.8, at 37°C Plasmodium falciparum