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Literature summary for 2.7.1.31 extracted from
- D-Glycerate 3-kinase, the last unknown enzyme in the photorespiratory cycle in Arabidopsis, belongs to a novel kinase family (2005), Plant Cell, 17, 2413-2420.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| functional expression of His-tagged enzyme in Escherichia coli strain BL21, phylogenetic analysis | Saccharomyces cerevisiae |
| single-copy gene, partial DNA and amino acid sequence determination and analysis, functional expression of His-tagged enzyme in Escherichia coli strain BL21, phylogenetic analysis | Arabidopsis thaliana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of inactive T-DNA insertion knockout mutants, which are not viable in normal air, but grow under elevated CO2 concentration, and show high accumulation of glycerate, overview | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ammonium sulfate | inhibits at high concentrations | Arabidopsis thaliana |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
x * 39000, native enzyme, SDS-PAGE | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-glycerate | Saccharomyces cerevisiae | concluding reaction of the photorespiratory C2 cycle essential for living in oxygenic atmosphere, overview | ADP + 3-phospho-D-glycerate | - |
? | |
| ATP + D-glycerate | Arabidopsis thaliana | concluding reaction of the photorespiratory C2 cycle essential for plants to live in oxygenic atmosphere, overview | ADP + 3-phospho-D-glycerate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
single-copy gene | - |
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 867fold from leaves by hydrophobic interaction, anion exchange, and 2 steps affinity chromatography, recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography | Arabidopsis thaliana |
| recombinant His-tagged enzyme from Escherichia coli strain BL21 to homogeneity by nickel affinity chromatography | Saccharomyces cerevisiae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Arabidopsis thaliana | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 21.3 | - |
purified recombinant enzyme | Saccharomyces cerevisiae |
| 27.9 | - |
purified recombinant enzyme | Arabidopsis thaliana |
| 54.6 | - |
purified native enzyme | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-glycerate | - |
Saccharomyces cerevisiae | ADP + 3-phospho-D-glycerate | - |
? | |
| ATP + D-glycerate | concluding reaction of the photorespiratory C2 cycle essential for living in oxygenic atmosphere, overview | Saccharomyces cerevisiae | ADP + 3-phospho-D-glycerate | - |
? | |
| ATP + D-glycerate | concluding reaction of the photorespiratory C2 cycle essential for plants to live in oxygenic atmosphere, overview | Arabidopsis thaliana | ADP + 3-phospho-D-glycerate | - |
? | |
| ATP + D-glycerate | the enzyme contains an ATP/GTP binding site motif A, a P-loop, domain | Arabidopsis thaliana | ADP + 3-phospho-D-glycerate | - |
? | |
| additional information | no activity with uridine and ribulose 5-phosphate | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | no activity with uridine and ribulose 5-phosphate | Arabidopsis thaliana | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 39000, native enzyme, SDS-PAGE | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-glycerate kinase | - |
Saccharomyces cerevisiae |
| D-glycerate kinase | - |
Arabidopsis thaliana |
| GLYK | - |
Saccharomyces cerevisiae |
| GLYK | - |
Arabidopsis thaliana |
| More | the enzyme belongs to the glycerate kinase family of enzymes | Saccharomyces cerevisiae |
| More | the enzyme belongs to the glycerate kinase family of enzymes | Arabidopsis thaliana |
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Release 2023.1 (January 2023)
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