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Literature summary for 2.7.1.30 extracted from

  • Shimada, K.; Kato, H.; Miyata, H.; Ikawa, M.
    Glycerol kinase 2 is essential for proper arrangement of crescent-like mitochondria to form the mitochondrial sheath during mouse spermatogenesis (2019), J. Reprod. Dev., 65, 155-162 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene Gk2, a single exon gene Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information generation of Gk2-7/-7 KO mice using the CRISPR/Cas9 system, analysis of sperm mitochondria in testis using a freeze-fracture method with scanning electron microscopy. To generate the Gk2 KO mice, the pX330 plasmid is prepared expressing a chimeric sgRNA together with human codon-optimized Cas9 (hCas9) by ligating oligonucleotides into the BbsI site. The sgRNAs recognize sequences close to the start codon. Gk2-disrupted spermatids show abnormal localization of crescent-like mitochondria, in spite of the initial proper alignment of spherical mitochondria around the flagellum, which causes abnormal mitochondrial sheath formation leading to exposure of the outer dense fibers. Phenotype of Gk2-7/-7 mice, overview Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Mus musculus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + glycerol Mus musculus
-
ADP + sn-glycerol 3-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus Q9WU65 B6D2F1 (C57BL/6 × DBA2) mice or ICR mice, and B6D2-Tg(CAG/ Su9-DsRed2, Acr3-EGFP)RBGS002Osb (RBGS, Red Body Green Sperm) mice
-

Source Tissue

Source Tissue Comment Organism Textmining
semen
-
Mus musculus
-
spermatid
-
Mus musculus
-
spermatozoon
-
Mus musculus
-
testis
-
Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + glycerol
-
Mus musculus ADP + sn-glycerol 3-phosphate
-
?

Synonyms

Synonyms Comment Organism
GK2
-
Mus musculus
glycerol kinase 2
-
Mus musculus

General Information

General Information Comment Organism
evolution both Gykl1 and Gk2 are thought to have arisen by the transposition of Gk located on the X chromosome, and have high homology with Gk. But both Gykl1 and Gk2 show testis-specific expression, and the proteins have no glycerol kinase activity in vitro, unlike GK and GK5 Mus musculus
malfunction Gk2-deficient mice show male infertility with disordered mitochondrial sheath formation. Gk2 disrupted mice are male infertile because their spermatozoa cannot pass through the uterotubal junction (UTJ) due to reduced motility. Disorganization of the mitochondrial sheath occurs in glycerol kinase 2 (Gk2) disrupted mice Mus musculus
physiological function enzyme glycerol kinase 2 (GK2) is essential for proper arrangement of crescent-like mitochondria to form the mitochondrial sheath during mouse spermatogenesis Mus musculus