Cloned (Comment) | Organism |
---|---|
gene Gk2, a single exon gene | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of Gk2-7/-7 KO mice using the CRISPR/Cas9 system, analysis of sperm mitochondria in testis using a freeze-fracture method with scanning electron microscopy. To generate the Gk2 KO mice, the pX330 plasmid is prepared expressing a chimeric sgRNA together with human codon-optimized Cas9 (hCas9) by ligating oligonucleotides into the BbsI site. The sgRNAs recognize sequences close to the start codon. Gk2-disrupted spermatids show abnormal localization of crescent-like mitochondria, in spite of the initial proper alignment of spherical mitochondria around the flagellum, which causes abnormal mitochondrial sheath formation leading to exposure of the outer dense fibers. Phenotype of Gk2-7/-7 mice, overview | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Mus musculus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + glycerol | Mus musculus | - |
ADP + sn-glycerol 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q9WU65 | B6D2F1 (C57BL/6 × DBA2) mice or ICR mice, and B6D2-Tg(CAG/ Su9-DsRed2, Acr3-EGFP)RBGS002Osb (RBGS, Red Body Green Sperm) mice | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
semen | - |
Mus musculus | - |
spermatid | - |
Mus musculus | - |
spermatozoon | - |
Mus musculus | - |
testis | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + glycerol | - |
Mus musculus | ADP + sn-glycerol 3-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GK2 | - |
Mus musculus |
glycerol kinase 2 | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
evolution | both Gykl1 and Gk2 are thought to have arisen by the transposition of Gk located on the X chromosome, and have high homology with Gk. But both Gykl1 and Gk2 show testis-specific expression, and the proteins have no glycerol kinase activity in vitro, unlike GK and GK5 | Mus musculus |
malfunction | Gk2-deficient mice show male infertility with disordered mitochondrial sheath formation. Gk2 disrupted mice are male infertile because their spermatozoa cannot pass through the uterotubal junction (UTJ) due to reduced motility. Disorganization of the mitochondrial sheath occurs in glycerol kinase 2 (Gk2) disrupted mice | Mus musculus |
physiological function | enzyme glycerol kinase 2 (GK2) is essential for proper arrangement of crescent-like mitochondria to form the mitochondrial sheath during mouse spermatogenesis | Mus musculus |