Any feedback?
Literature summary for 2.7.1.30 extracted from
- An improved amperometric triglyceride biosensor based on co-immobilization of nanoparticles of lipase, glycerol kinase and glycerol 3-phosphate oxidase onto pencil graphite electrode (2017), Enzyme Microb. Technol., 100, 11-16 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | co-immobilization of nanoparticles of lipase, glycerol kinase and glycerol 3-phosphate oxidase onto pencil graphite electrode, function as improved amperometric triglyceride biosensor. An improved amperometric triglyceride (TG) biosensor is fabricated using lipaseNPs/GKNPs/GPONPs/PG electrode as the working electrode, Ag/AgCl as the standard electrode and Pt wire as auxiliary electrode. The biosensor shows optimum response within 2.5 s at pH 7.0 and temperature of 35°C. The biosensor measures current due to electrons generated at 0.1 V against Ag/AgCl, from H2O2, which is produced from triolein by co-immobilized enzyme nanoparticles (ENPs). The biosensor is evaluated and employed for determination of triglyceride in the serum of apparently healthy subject and persons suffering from hypertriglyceridemia. The biosensor loses 20% of its initial activity after continued uses over a period of 240 days, while being stored at 4°C. Construction of the biosensor, evaluation, and optimization, overview | Cellulomonas sp. |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Cellulomonas sp. |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 128000 | - |
gel filtration | Cellulomonas sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + glycerol | Cellulomonas sp. | - |
ADP + sn-glycerol 3-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cellulomonas sp. | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Cellulomonas sp. | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 25 | 75 | purified enzyme, pH 9.8, 25°C | Cellulomonas sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + glycerol | - |
Cellulomonas sp. | ADP + sn-glycerol 3-phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
enzyme in biosensor on graphite electrode | Cellulomonas sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
enzyme in biosensor on graphite electrode | Cellulomonas sp. |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
