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Literature summary for 2.7.1.30 extracted from
- Enhancement of lipid production in Synechocystis sp. PCC 6803 overexpressing glycerol kinase under oxidative stress with glycerol supplementation (2018), Biores. Technol., 267, 532-540 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene slr1672 or glpK, recombinant overexpression in Synechocystis sp. PCC 6803, subcloning in Escherichia coli strain DH5alpha, quantitative RT-PCR enzyme expression analysis | Synechocystis sp. PCC 6803 |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | overexpression of glycerol kinase under oxidative stress with glycerol supplementation leads to enhancement of lipid production in Synechocystis sp. PCC 6803 | Synechocystis sp. PCC 6803 |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Synechocystis sp. PCC 6803 |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + glycerol | Synechocystis sp. PCC 6803 | - |
ADP + sn-glycerol 3-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Synechocystis sp. PCC 6803 | P74260 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.4 | - |
recombinant enzyme-overexpressing and phenol-treated Synechocystis sp. PCC 6803, pH and temperature not specified in the publication | Synechocystis sp. PCC 6803 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + glycerol | - |
Synechocystis sp. PCC 6803 | ADP + sn-glycerol 3-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GLPK | - |
Synechocystis sp. PCC 6803 |
| slr1672 | - |
Synechocystis sp. PCC 6803 |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Synechocystis sp. PCC 6803 | phenol treatment increases the glycerol kinase activity in the overexpressing strains whereas increasing concentrations of phenol decrease the glycerol kinase activity in the wild-type strain | down |
| Synechocystis sp. PCC 6803 | RT-PCR analysis reveals that the expression of glpK is upregulated from 1.3 to 2.4 and from 1.89 to 3.64fold after phenol treatment and glycerol supplementation, respectively, overview. Phenol treatment increases the glycerol kinase activity in the overexpressing strains whereas increasing concentrations of phenol decrease the glycerol kinase activity in the wild-type strain | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | overexpression of glycerol kinase under oxidative stress with glycerol supplementation leads to enhancement of lipid production in Synechocystis sp. PCC 6803 | Synechocystis sp. PCC 6803 |
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