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Literature summary for 2.7.1.30 extracted from

  • Koga, Y.; Morikawa, M.; Haruki, M.; Nakamura, H.; Imanaka, T.; Kanaya, S.
    Thermostable glycerol kinase from a hyperthermophilic archaeon: gene cloning and characterization of the recombinant enzyme (1998), Protein Eng., 11, 1219-1227.
    View publication on PubMed
Search for more literature for 2.7.1.30

Cloned(Commentary)

Cloned (Comment) Organism
-
 Thermococcus kodakarensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 ATP pH 8.0, 80°C Thermococcus kodakarensis
0.111
-
 glycerol pH 8.0, 80°C Thermococcus kodakarensis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ best effect, may be replaced by Zn2+ Thermococcus kodakarensis
Zn2+ can substitute for Co2+ Thermococcus kodakarensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 2 * 56000, SDS-PAGE, deduced from gene sequence Thermococcus kodakarensis

Organism

Organism UniProt Comment Textmining
Thermococcus kodakarensis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + glycerol weak: GTP, CTP, UTP, ITP Thermococcus kodakarensis ADP + sn-glycerol 3-phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 56000, SDS-PAGE, deduced from gene sequence Thermococcus kodakarensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Thermococcus kodakarensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
 30 min, loss of 50% of activity Thermococcus kodakarensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Thermococcus kodakarensis