BRENDA - Enzyme Database show
show all sequences of 2.7.1.30

Glycerol and glycerate kinases

Thorner, J.W.; Paulus, H.; The Enzymes,3rd ed. (Boyer,P. D. ,ed. ) 8, 487-508 (1973)
No PubMed abstract available

Data extracted from this reference:

General Stability
General Stability
Organism
glycerol affords considerable stabilization at the unfavorable pH values
Mycobacterium sp.
pigeon liver enzyme is sensitive to extreme dilution but can be stabilized by addition of 0.01% bovine serum albumin
Columba sp.
the enzyme in crude extracts is stable to freezing and thawing, while more purified preparations are inactivated
Rattus norvegicus
the enzyme in crude extracts is stable to freezing and thawing, while more purified preparations are inactivated
Pseudomonas aeruginosa
Inhibitors
Inhibitors
Commentary
Organism
Structure
ADP
-
Rattus norvegicus
ADP
-
Gallus gallus
ADP
-
Escherichia coli
AMP
-
Saccharomyces cerevisiae
fructose 1,6-diphosphate
not
Candida mycoderma
fructose 1,6-diphosphate
not
Neurospora crassa
fructose 1,6-diphosphate
-
Escherichia coli
glycerol 3-phosphate
-
Bacillus subtilis
glycerol 3-phosphate
-
Bombus sp.
glycerol 3-phosphate
-
Bos taurus
glycerol 3-phosphate
-
Candida mycoderma
glycerol 3-phosphate
-
Cavia porcellus
glycerol 3-phosphate
-
Clostridium novyi
glycerol 3-phosphate
-
Columba sp.
glycerol 3-phosphate
-
Cyberlindnera jadinii
glycerol 3-phosphate
-
Enterococcus faecalis
glycerol 3-phosphate
-
Escherichia coli
glycerol 3-phosphate
-
Felis catus
glycerol 3-phosphate
-
Geotrichum candidum
glycerol 3-phosphate
-
Gluconobacter oxydans
glycerol 3-phosphate
-
Halobacterium salinarum
glycerol 3-phosphate
-
Homo sapiens
glycerol 3-phosphate
-
Klebsiella aerogenes
glycerol 3-phosphate
-
Locusta sp.
glycerol 3-phosphate
-
Mesocricetus auratus
glycerol 3-phosphate
-
Mus musculus
glycerol 3-phosphate
-
Mycobacterium butyricum
glycerol 3-phosphate
-
Mycobacterium sp.
glycerol 3-phosphate
-
Mycobacterium tuberculosis
glycerol 3-phosphate
-
Mycolicibacterium smegmatis
glycerol 3-phosphate
-
Neurospora crassa
glycerol 3-phosphate
-
Nocardia asteroides
glycerol 3-phosphate
-
Oryctolagus cuniculus
glycerol 3-phosphate
-
Pseudomonas aeruginosa
glycerol 3-phosphate
competitive to glycerol
Rattus norvegicus
glycerol 3-phosphate
-
Saccharomyces cerevisiae
glycerol 3-phosphate
-
Shigella sonnei
glycerol 3-phosphate
-
Staphylococcus aureus
glycerol 3-phosphate
-
trout
glycerol 3-phosphate
-
Wickerhamomyces anomalus
iodoacetamide
-
Candida mycoderma
iodoacetamide
-
Rattus norvegicus
iodoacetate
-
Escherichia coli
N-ethylmaleimide
-
Rattus norvegicus
N-ethylmaleimide
-
Escherichia coli
p-chloromercuribenzoate
-
Candida mycoderma
p-chloromercuribenzoate
-
Rattus norvegicus
p-Hydroxymercuriphenylsulfonate
-
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Bos taurus
additional information
-
additional information
-
Candida mycoderma
additional information
-
additional information
-
Columba sp.
additional information
-
additional information
-
Escherichia coli
additional information
-
additional information
-
Gallus gallus
additional information
-
additional information
-
Mus musculus
additional information
-
additional information
-
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
described in the most tissues
Bacillus subtilis
5829
-
cytosol
described in the most tissues
Bombus sp.
5829
-
cytosol
described in the most tissues
Bos taurus
5829
-
cytosol
described in the most tissues
Candida mycoderma
5829
-
cytosol
described in the most tissues
Cavia porcellus
5829
-
cytosol
described in the most tissues
Clostridium novyi
5829
-
cytosol
described in the most tissues
Columba sp.
5829
-
cytosol
described in the most tissues
Cyberlindnera jadinii
5829
-
cytosol
described in the most tissues
Enterococcus faecalis
5829
-
cytosol
described in the most tissues
Escherichia coli
5829
-
cytosol
described in the most tissues
Felis catus
5829
-
cytosol
described in the most tissues
Geotrichum candidum
5829
-
cytosol
described in the most tissues
Gluconobacter oxydans
5829
-
cytosol
described in the most tissues
Halobacterium salinarum
5829
-
cytosol
described in the most tissues
Homo sapiens
5829
-
cytosol
described in the most tissues
Klebsiella aerogenes
5829
-
cytosol
described in the most tissues
Locusta sp.
5829
-
cytosol
described in the most tissues
Mesocricetus auratus
5829
-
cytosol
described in the most tissues
Mus musculus
5829
-
cytosol
described in the most tissues
Mycobacterium butyricum
5829
-
cytosol
described in the most tissues
Mycobacterium sp.
5829
-
cytosol
described in the most tissues
Mycobacterium tuberculosis
5829
-
cytosol
described in the most tissues
Mycolicibacterium smegmatis
5829
-
cytosol
described in the most tissues
Neurospora crassa
5829
-
cytosol
described in the most tissues
Nocardia asteroides
5829
-
cytosol
described in the most tissues
Oryctolagus cuniculus
5829
-
cytosol
described in the most tissues
Pseudomonas aeruginosa
5829
-
cytosol
described in the most tissues
Rattus norvegicus
5829
-
cytosol
described in the most tissues
Saccharomyces cerevisiae
5829
-
cytosol
described in the most tissues
Shigella sonnei
5829
-
cytosol
described in the most tissues
Staphylococcus aureus
5829
-
cytosol
described in the most tissues
trout
5829
-
cytosol
described in the most tissues
Wickerhamomyces anomalus
5829
-
mitochondrion
-
Bos taurus
5739
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
4 * 60000, SDS-PAGE
Saccharomyces cerevisiae
140000
-
gel filtration, zone sedimentation in sucrose density gradient
Neurospora crassa
210000
217000
equilibrium sedimentation
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + glycerol
Escherichia coli
enzyme functions primarily in the utilization of glycerol as a carbon and energy source
?
-
-
-
ATP + glycerol
Mesocricetus auratus
higher organisms: salvage of glycerol released upon lipolysis
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus subtilis
-
-
-
Bombus sp.
-
queen bumblebee
-
Bos taurus
-
-
-
Candida mycoderma
-
-
-
Cavia porcellus
-
-
-
Clostridium novyi
-
-
-
Columba sp.
-
pigeon
-
Cyberlindnera jadinii
-
-
-
Enterococcus faecalis
-
-
-
Escherichia coli
-
-
-
Felis catus
-
-
-
Gallus gallus
-
-
-
Geotrichum candidum
-
-
-
Gluconobacter oxydans
-
-
-
Halobacterium salinarum
-
-
-
Homo sapiens
-
-
-
Klebsiella aerogenes
-
-
-
Locusta sp.
-
-
-
Mesocricetus auratus
-
-
-
Mus musculus
-
-
-
Mycobacterium butyricum
-
-
-
Mycobacterium sp.
-
607
-
Mycobacterium sp. 607
-
607
-
Mycobacterium tuberculosis
-
-
-
Mycolicibacterium smegmatis
-
-
-
Neurospora crassa
-
-
-
Nocardia asteroides
-
-
-
Oryctolagus cuniculus
-
-
-
Pseudomonas aeruginosa
-
-
-
Rattus norvegicus
-
-
-
Saccharomyces cerevisiae
-
-
-
Shigella sonnei
-
-
-
Staphylococcus aureus
-
-
-
trout
-
-
-
Wickerhamomyces anomalus
-
-
-
Oxidation Stability
Oxidation Stability
Organism
susceptible to inactivation by oxidation of sulfhydryl groups
Columba sp.
Reaction
Reaction
Commentary
Organism
ATP + glycerol = ADP + sn-glycerol 3-phosphate
ordered mechanism
Escherichia coli
ATP + glycerol = ADP + sn-glycerol 3-phosphate
ordered mechanism
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
adipose tissue
-
Gallus gallus
-
adipose tissue
white and brown
Homo sapiens
-
adipose tissue
white and brown
Locusta sp.
-
adipose tissue
white and brown
Mus musculus
-
adipose tissue
white and brown
Oryctolagus cuniculus
-
adipose tissue
white and brown
Rattus norvegicus
-
adipose tissue
-
Bos taurus
-
intestine
-
Felis catus
-
intestine
-
Mesocricetus auratus
-
intestine
-
Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
liver
-
Columba sp.
-
liver
-
Mesocricetus auratus
-
liver
-
Mus musculus
-
liver
-
Rattus norvegicus
-
liver
-
trout
-
liver
-
Gallus gallus
-
liver
-
Bos taurus
-
additional information
enzyme activity in various tissues
Bacillus subtilis
-
additional information
enzyme activity in various tissues
Bombus sp.
-
additional information
enzyme activity in various tissues
Bos taurus
-
additional information
enzyme activity in various tissues
Candida mycoderma
-
additional information
enzyme activity in various tissues
Cavia porcellus
-
additional information
enzyme activity in various tissues
Clostridium novyi
-
additional information
enzyme activity in various tissues
Columba sp.
-
additional information
enzyme activity in various tissues
Cyberlindnera jadinii
-
additional information
enzyme activity in various tissues
Enterococcus faecalis
-
additional information
enzyme activity in various tissues
Escherichia coli
-
additional information
enzyme activity in various tissues
Felis catus
-
additional information
enzyme activity in various tissues
Geotrichum candidum
-
additional information
enzyme activity in various tissues
Gluconobacter oxydans
-
additional information
enzyme activity in various tissues
Halobacterium salinarum
-
additional information
enzyme activity in various tissues
Homo sapiens
-
additional information
enzyme activity in various tissues
Klebsiella aerogenes
-
additional information
enzyme activity in various tissues
Locusta sp.
-
additional information
enzyme activity in various tissues
Mesocricetus auratus
-
additional information
enzyme activity in various tissues
Mus musculus
-
additional information
enzyme activity in various tissues
Mycobacterium butyricum
-
additional information
enzyme activity in various tissues
Mycobacterium sp.
-
additional information
enzyme activity in various tissues
Mycobacterium tuberculosis
-
additional information
enzyme activity in various tissues
Mycolicibacterium smegmatis
-
additional information
enzyme activity in various tissues
Neurospora crassa
-
additional information
enzyme activity in various tissues
Nocardia asteroides
-
additional information
enzyme activity in various tissues
Oryctolagus cuniculus
-
additional information
enzyme activity in various tissues
Pseudomonas aeruginosa
-
additional information
enzyme activity in various tissues
Rattus norvegicus
-
additional information
enzyme activity in various tissues
Saccharomyces cerevisiae
-
additional information
enzyme activity in various tissues
Shigella sonnei
-
additional information
enzyme activity in various tissues
Staphylococcus aureus
-
additional information
enzyme activity in various tissues
trout
-
additional information
enzyme activity in various tissues
Wickerhamomyces anomalus
-
muscle
-
Bombus sp.
-
muscle
-
Columba sp.
-
muscle
-
Gallus gallus
-
muscle
-
Locusta sp.
-
muscle
-
Oryctolagus cuniculus
-
muscle
-
Rattus norvegicus
-
muscle
-
trout
-
spermatozoon
-
Bos taurus
-
spermatozoon
-
Gallus gallus
-
spermatozoon
-
Homo sapiens
-
spermatozoon
-
Oryctolagus cuniculus
-
spermatozoon
-
trout
-
Storage Stability
Storage Stability
Organism
-20°C, pH 5.0, partially purified enzymes have half-lives of several weeks to months
Rattus norvegicus
-20°C, pH 5.0, partially purified enzymes have half-lives of several weeks to months
Mesocricetus auratus
-20°C, pH 5.0, partially purified enzymes have half-lives of several weeks to months
Columba sp.
as crystalline suspension in saturated ammonium sulfate, solutions containing 10 mM glycerol, 1 mM EDTA and a thiol e.g. 2-mercaptoethanol, yeast enzyme stable for several months, E. coli enzyme for several years
Escherichia coli
as crystalline suspension in saturated ammonium sulfate, solutions containing 10 mM glycerol, 1 mM EDTA and a thiol e.g. 2-mercaptoethanol, yeast enzyme stable for several months, E. coli enzyme for several years
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + D-glyceraldehyde
-
641287
Escherichia coli
ADP + D-glyceraldehyde 3-phosphate
-
-
-
?
ATP + dihydroxyacetone
-
641287
Escherichia coli
ADP + dihydroxyacetone phosphate
-
-
-
?
ATP + dihydroxyacetone
-
641287
Candida mycoderma
ADP + dihydroxyacetone phosphate
-
-
-
?
ATP + glycerol
-
641287
Gallus gallus
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Cavia porcellus
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Staphylococcus aureus
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Bacillus subtilis
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Cyberlindnera jadinii
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Mus musculus
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Escherichia coli
ADP + sn-glycerol 3-phosphate
-
-
-
-
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Homo sapiens
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Rattus norvegicus
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Saccharomyces cerevisiae
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Bos taurus
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Oryctolagus cuniculus
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Mycolicibacterium smegmatis
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Neurospora crassa
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Klebsiella aerogenes
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Pseudomonas aeruginosa
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Halobacterium salinarum
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Enterococcus faecalis
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Mycobacterium tuberculosis
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Mesocricetus auratus
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Felis catus
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Mycobacterium sp.
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Nocardia asteroides
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Gluconobacter oxydans
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Mycobacterium butyricum
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Wickerhamomyces anomalus
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Shigella sonnei
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
trout
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Geotrichum candidum
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Columba sp.
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Locusta sp.
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Bombus sp.
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Candida mycoderma
ADP + sn-glycerol 3-phosphate
-
-
-
-
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Clostridium novyi
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Mycobacterium sp. 607
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme functions primarily in the utilization of glycerol as a carbon and energy source
641287
Escherichia coli
?
-
-
-
-
ATP + glycerol
higher organisms: salvage of glycerol released upon lipolysis
641287
Mesocricetus auratus
?
-
-
-
-
ATP + L-glyceraldehyde
-
641287
Escherichia coli
ADP + L-glyceraldehyde 3-phosphate
-
-
-
?
ATP + L-glyceraldehyde
-
641287
Candida mycoderma
ADP + L-glyceraldehyde 3-phosphate
-
-
-
?
CTP + glycerol
-
641287
Gallus gallus
CDP + glycerol 3-phosphate
-
-
-
?
CTP + glycerol
-
641287
Candida mycoderma
CDP + glycerol 3-phosphate
-
-
-
?
ITP + glycerol
-
641287
Gallus gallus
IDP + glycerol 3-phosphate
-
-
-
?
ITP + glycerol
-
641287
Candida mycoderma
IDP + glycerol 3-phosphate
-
-
-
?
additional information
-
641287
Saccharomyces cerevisiae
?
-
-
-
-
additional information
-
641287
Candida mycoderma
?
-
-
-
-
additional information
phosphate rather than D-glyceraldehyde 3-phosphate is formed, the hydrated form of this triose is phosphorylated in position 1 to yield an unstable intermediate that decomposes to D-glyceraldehyde + phosphate
641287
Escherichia coli
?
-
-
-
-
UTP + glycerol
-
641287
Gallus gallus
UDP + glycerol 3-phosphate
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
4 * 55000-57000, equilibrium ultracentrifugation in presence of 6 M guanidine HCl, SDS-PAGE
Escherichia coli
tetramer
4 * 60000, SDS-PAGE
Saccharomyces cerevisiae
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
-
Escherichia coli
additional information
-
enzyme is quite heat labile
Mycobacterium sp.
additional information
-
-
Rattus norvegicus
50
-
5 min, 30% loss of activity
Neurospora crassa
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Bacillus subtilis
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Bombus sp.
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Bos taurus
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Candida mycoderma
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Cavia porcellus
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Clostridium novyi
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Columba sp.
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Cyberlindnera jadinii
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Enterococcus faecalis
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Escherichia coli
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Felis catus
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Geotrichum candidum
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Gluconobacter oxydans
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Halobacterium salinarum
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Homo sapiens
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Klebsiella aerogenes
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Locusta sp.
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Mesocricetus auratus
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Mus musculus
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Mycobacterium butyricum
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Mycobacterium sp.
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Mycobacterium tuberculosis
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Mycolicibacterium smegmatis
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Neurospora crassa
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Nocardia asteroides
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Oryctolagus cuniculus
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Pseudomonas aeruginosa
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Rattus norvegicus
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Saccharomyces cerevisiae
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Shigella sonnei
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Staphylococcus aureus
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
trout
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Wickerhamomyces anomalus
70
-
-
Bacillus subtilis
70
-
-
Bombus sp.
70
-
-
Bos taurus
70
-
-
Candida mycoderma
70
-
-
Cavia porcellus
70
-
-
Clostridium novyi
70
-
-
Columba sp.
70
-
-
Cyberlindnera jadinii
70
-
-
Enterococcus faecalis
70
-
t1/2: 5 min
Escherichia coli
70
-
-
Felis catus
70
-
-
Geotrichum candidum
70
-
-
Gluconobacter oxydans
70
-
-
Halobacterium salinarum
70
-
-
Homo sapiens
70
-
-
Klebsiella aerogenes
70
-
-
Locusta sp.
70
-
-
Mesocricetus auratus
70
-
-
Mus musculus
70
-
-
Mycobacterium butyricum
70
-
-
Mycobacterium sp.
70
-
-
Mycobacterium tuberculosis
70
-
-
Mycolicibacterium smegmatis
70
-
-
Neurospora crassa
70
-
-
Nocardia asteroides
70
-
-
Oryctolagus cuniculus
70
-
-
Pseudomonas aeruginosa
70
-
-
Rattus norvegicus
70
-
-
Saccharomyces cerevisiae
70
-
-
Shigella sonnei
70
-
-
Staphylococcus aureus
70
-
-
trout
70
-
-
Wickerhamomyces anomalus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
7.5
-
Clostridium novyi
7
7.5
-
Mycobacterium sp.
8
-
-
Saccharomyces cerevisiae
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
additional information
-
glycerol affords considerable stabilization at the unfavorable pH values, glycerol kinases from microorganisms most stable at a neutral pH, glycerol kinases from higher organisms most stable in an acidic pH range
Rattus norvegicus
5
-
0°C, 6 h, without glycerol, completely stable
Columba sp.
5
-
0°C, 24 h, complete loss of activity, 30°C, 30 min, 75% loss of activity
Escherichia coli
6
9
30°C, in presence of glycerol, completely stable for 30 min
Mycobacterium sp.
7
-
0°C, 6 h, without glycerol, 90% loss of activity
Columba sp.
7
-
0°C, 24 h, 50% loss of activity
Escherichia coli
7.5
-
25°C, no loss of activity after several h
Rattus norvegicus
9.8
-
25°C, half-life: 6.5 min
Rattus norvegicus
General Stability (protein specific)
General Stability
Organism
glycerol affords considerable stabilization at the unfavorable pH values
Mycobacterium sp.
pigeon liver enzyme is sensitive to extreme dilution but can be stabilized by addition of 0.01% bovine serum albumin
Columba sp.
the enzyme in crude extracts is stable to freezing and thawing, while more purified preparations are inactivated
Rattus norvegicus
the enzyme in crude extracts is stable to freezing and thawing, while more purified preparations are inactivated
Pseudomonas aeruginosa
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ADP
-
Rattus norvegicus
ADP
-
Gallus gallus
ADP
-
Escherichia coli
AMP
-
Saccharomyces cerevisiae
fructose 1,6-diphosphate
not
Candida mycoderma
fructose 1,6-diphosphate
not
Neurospora crassa
fructose 1,6-diphosphate
-
Escherichia coli
glycerol 3-phosphate
-
Bacillus subtilis
glycerol 3-phosphate
-
Bombus sp.
glycerol 3-phosphate
-
Bos taurus
glycerol 3-phosphate
-
Candida mycoderma
glycerol 3-phosphate
-
Cavia porcellus
glycerol 3-phosphate
-
Clostridium novyi
glycerol 3-phosphate
-
Columba sp.
glycerol 3-phosphate
-
Cyberlindnera jadinii
glycerol 3-phosphate
-
Enterococcus faecalis
glycerol 3-phosphate
-
Escherichia coli
glycerol 3-phosphate
-
Felis catus
glycerol 3-phosphate
-
Geotrichum candidum
glycerol 3-phosphate
-
Gluconobacter oxydans
glycerol 3-phosphate
-
Halobacterium salinarum
glycerol 3-phosphate
-
Homo sapiens
glycerol 3-phosphate
-
Klebsiella aerogenes
glycerol 3-phosphate
-
Locusta sp.
glycerol 3-phosphate
-
Mesocricetus auratus
glycerol 3-phosphate
-
Mus musculus
glycerol 3-phosphate
-
Mycobacterium butyricum
glycerol 3-phosphate
-
Mycobacterium sp.
glycerol 3-phosphate
-
Mycobacterium tuberculosis
glycerol 3-phosphate
-
Mycolicibacterium smegmatis
glycerol 3-phosphate
-
Neurospora crassa
glycerol 3-phosphate
-
Nocardia asteroides
glycerol 3-phosphate
-
Oryctolagus cuniculus
glycerol 3-phosphate
-
Pseudomonas aeruginosa
glycerol 3-phosphate
competitive to glycerol
Rattus norvegicus
glycerol 3-phosphate
-
Saccharomyces cerevisiae
glycerol 3-phosphate
-
Shigella sonnei
glycerol 3-phosphate
-
Staphylococcus aureus
glycerol 3-phosphate
-
trout
glycerol 3-phosphate
-
Wickerhamomyces anomalus
iodoacetamide
-
Candida mycoderma
iodoacetamide
-
Rattus norvegicus
iodoacetate
-
Escherichia coli
N-ethylmaleimide
-
Rattus norvegicus
N-ethylmaleimide
-
Escherichia coli
p-chloromercuribenzoate
-
Candida mycoderma
p-chloromercuribenzoate
-
Rattus norvegicus
p-Hydroxymercuriphenylsulfonate
-
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Bos taurus
additional information
-
additional information
-
Candida mycoderma
additional information
-
additional information
-
Columba sp.
additional information
-
additional information
-
Escherichia coli
additional information
-
additional information
-
Gallus gallus
additional information
-
additional information
-
Mus musculus
additional information
-
additional information
-
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
described in the most tissues
Bacillus subtilis
5829
-
cytosol
described in the most tissues
Bombus sp.
5829
-
cytosol
described in the most tissues
Bos taurus
5829
-
cytosol
described in the most tissues
Candida mycoderma
5829
-
cytosol
described in the most tissues
Cavia porcellus
5829
-
cytosol
described in the most tissues
Clostridium novyi
5829
-
cytosol
described in the most tissues
Columba sp.
5829
-
cytosol
described in the most tissues
Cyberlindnera jadinii
5829
-
cytosol
described in the most tissues
Enterococcus faecalis
5829
-
cytosol
described in the most tissues
Escherichia coli
5829
-
cytosol
described in the most tissues
Felis catus
5829
-
cytosol
described in the most tissues
Geotrichum candidum
5829
-
cytosol
described in the most tissues
Gluconobacter oxydans
5829
-
cytosol
described in the most tissues
Halobacterium salinarum
5829
-
cytosol
described in the most tissues
Homo sapiens
5829
-
cytosol
described in the most tissues
Klebsiella aerogenes
5829
-
cytosol
described in the most tissues
Locusta sp.
5829
-
cytosol
described in the most tissues
Mesocricetus auratus
5829
-
cytosol
described in the most tissues
Mus musculus
5829
-
cytosol
described in the most tissues
Mycobacterium butyricum
5829
-
cytosol
described in the most tissues
Mycobacterium sp.
5829
-
cytosol
described in the most tissues
Mycobacterium tuberculosis
5829
-
cytosol
described in the most tissues
Mycolicibacterium smegmatis
5829
-
cytosol
described in the most tissues
Neurospora crassa
5829
-
cytosol
described in the most tissues
Nocardia asteroides
5829
-
cytosol
described in the most tissues
Oryctolagus cuniculus
5829
-
cytosol
described in the most tissues
Pseudomonas aeruginosa
5829
-
cytosol
described in the most tissues
Rattus norvegicus
5829
-
cytosol
described in the most tissues
Saccharomyces cerevisiae
5829
-
cytosol
described in the most tissues
Shigella sonnei
5829
-
cytosol
described in the most tissues
Staphylococcus aureus
5829
-
cytosol
described in the most tissues
trout
5829
-
cytosol
described in the most tissues
Wickerhamomyces anomalus
5829
-
mitochondrion
-
Bos taurus
5739
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
4 * 60000, SDS-PAGE
Saccharomyces cerevisiae
140000
-
gel filtration, zone sedimentation in sucrose density gradient
Neurospora crassa
210000
217000
equilibrium sedimentation
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + glycerol
Escherichia coli
enzyme functions primarily in the utilization of glycerol as a carbon and energy source
?
-
-
-
ATP + glycerol
Mesocricetus auratus
higher organisms: salvage of glycerol released upon lipolysis
?
-
-
-
Oxidation Stability (protein specific)
Oxidation Stability
Organism
susceptible to inactivation by oxidation of sulfhydryl groups
Columba sp.
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
adipose tissue
-
Gallus gallus
-
adipose tissue
white and brown
Homo sapiens
-
adipose tissue
white and brown
Locusta sp.
-
adipose tissue
white and brown
Mus musculus
-
adipose tissue
white and brown
Oryctolagus cuniculus
-
adipose tissue
white and brown
Rattus norvegicus
-
adipose tissue
-
Bos taurus
-
intestine
-
Felis catus
-
intestine
-
Mesocricetus auratus
-
intestine
-
Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
liver
-
Columba sp.
-
liver
-
Mesocricetus auratus
-
liver
-
Mus musculus
-
liver
-
Rattus norvegicus
-
liver
-
trout
-
liver
-
Gallus gallus
-
liver
-
Bos taurus
-
additional information
enzyme activity in various tissues
Bacillus subtilis
-
additional information
enzyme activity in various tissues
Bombus sp.
-
additional information
enzyme activity in various tissues
Bos taurus
-
additional information
enzyme activity in various tissues
Candida mycoderma
-
additional information
enzyme activity in various tissues
Cavia porcellus
-
additional information
enzyme activity in various tissues
Clostridium novyi
-
additional information
enzyme activity in various tissues
Columba sp.
-
additional information
enzyme activity in various tissues
Cyberlindnera jadinii
-
additional information
enzyme activity in various tissues
Enterococcus faecalis
-
additional information
enzyme activity in various tissues
Escherichia coli
-
additional information
enzyme activity in various tissues
Felis catus
-
additional information
enzyme activity in various tissues
Geotrichum candidum
-
additional information
enzyme activity in various tissues
Gluconobacter oxydans
-
additional information
enzyme activity in various tissues
Halobacterium salinarum
-
additional information
enzyme activity in various tissues
Homo sapiens
-
additional information
enzyme activity in various tissues
Klebsiella aerogenes
-
additional information
enzyme activity in various tissues
Locusta sp.
-
additional information
enzyme activity in various tissues
Mesocricetus auratus
-
additional information
enzyme activity in various tissues
Mus musculus
-
additional information
enzyme activity in various tissues
Mycobacterium butyricum
-
additional information
enzyme activity in various tissues
Mycobacterium sp.
-
additional information
enzyme activity in various tissues
Mycobacterium tuberculosis
-
additional information
enzyme activity in various tissues
Mycolicibacterium smegmatis
-
additional information
enzyme activity in various tissues
Neurospora crassa
-
additional information
enzyme activity in various tissues
Nocardia asteroides
-
additional information
enzyme activity in various tissues
Oryctolagus cuniculus
-
additional information
enzyme activity in various tissues
Pseudomonas aeruginosa
-
additional information
enzyme activity in various tissues
Rattus norvegicus
-
additional information
enzyme activity in various tissues
Saccharomyces cerevisiae
-
additional information
enzyme activity in various tissues
Shigella sonnei
-
additional information
enzyme activity in various tissues
Staphylococcus aureus
-
additional information
enzyme activity in various tissues
trout
-
additional information
enzyme activity in various tissues
Wickerhamomyces anomalus
-
muscle
-
Bombus sp.
-
muscle
-
Columba sp.
-
muscle
-
Gallus gallus
-
muscle
-
Locusta sp.
-
muscle
-
Oryctolagus cuniculus
-
muscle
-
Rattus norvegicus
-
muscle
-
trout
-
spermatozoon
-
Bos taurus
-
spermatozoon
-
Gallus gallus
-
spermatozoon
-
Homo sapiens
-
spermatozoon
-
Oryctolagus cuniculus
-
spermatozoon
-
trout
-
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, pH 5.0, partially purified enzymes have half-lives of several weeks to months
Rattus norvegicus
-20°C, pH 5.0, partially purified enzymes have half-lives of several weeks to months
Mesocricetus auratus
-20°C, pH 5.0, partially purified enzymes have half-lives of several weeks to months
Columba sp.
as crystalline suspension in saturated ammonium sulfate, solutions containing 10 mM glycerol, 1 mM EDTA and a thiol e.g. 2-mercaptoethanol, yeast enzyme stable for several months, E. coli enzyme for several years
Escherichia coli
as crystalline suspension in saturated ammonium sulfate, solutions containing 10 mM glycerol, 1 mM EDTA and a thiol e.g. 2-mercaptoethanol, yeast enzyme stable for several months, E. coli enzyme for several years
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + D-glyceraldehyde
-
641287
Escherichia coli
ADP + D-glyceraldehyde 3-phosphate
-
-
-
?
ATP + dihydroxyacetone
-
641287
Escherichia coli
ADP + dihydroxyacetone phosphate
-
-
-
?
ATP + dihydroxyacetone
-
641287
Candida mycoderma
ADP + dihydroxyacetone phosphate
-
-
-
?
ATP + glycerol
-
641287
Gallus gallus
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Cavia porcellus
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Staphylococcus aureus
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Bacillus subtilis
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Cyberlindnera jadinii
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Mus musculus
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Escherichia coli
ADP + sn-glycerol 3-phosphate
-
-
-
-
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Homo sapiens
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Rattus norvegicus
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Saccharomyces cerevisiae
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Bos taurus
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Oryctolagus cuniculus
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Mycolicibacterium smegmatis
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Neurospora crassa
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Klebsiella aerogenes
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Pseudomonas aeruginosa
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Halobacterium salinarum
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Enterococcus faecalis
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Mycobacterium tuberculosis
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Mesocricetus auratus
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Felis catus
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Mycobacterium sp.
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Nocardia asteroides
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Gluconobacter oxydans
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Mycobacterium butyricum
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Wickerhamomyces anomalus
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Shigella sonnei
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
trout
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Geotrichum candidum
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Columba sp.
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Locusta sp.
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Bombus sp.
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Candida mycoderma
ADP + sn-glycerol 3-phosphate
-
-
-
-
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Clostridium novyi
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme can utilize only ATP as phosphoryl group donor
641287
Mycobacterium sp. 607
ADP + sn-glycerol 3-phosphate
-
-
-
?
ATP + glycerol
enzyme functions primarily in the utilization of glycerol as a carbon and energy source
641287
Escherichia coli
?
-
-
-
-
ATP + glycerol
higher organisms: salvage of glycerol released upon lipolysis
641287
Mesocricetus auratus
?
-
-
-
-
ATP + L-glyceraldehyde
-
641287
Escherichia coli
ADP + L-glyceraldehyde 3-phosphate
-
-
-
?
ATP + L-glyceraldehyde
-
641287
Candida mycoderma
ADP + L-glyceraldehyde 3-phosphate
-
-
-
?
CTP + glycerol
-
641287
Gallus gallus
CDP + glycerol 3-phosphate
-
-
-
?
CTP + glycerol
-
641287
Candida mycoderma
CDP + glycerol 3-phosphate
-
-
-
?
ITP + glycerol
-
641287
Gallus gallus
IDP + glycerol 3-phosphate
-
-
-
?
ITP + glycerol
-
641287
Candida mycoderma
IDP + glycerol 3-phosphate
-
-
-
?
additional information
-
641287
Saccharomyces cerevisiae
?
-
-
-
-
additional information
-
641287
Candida mycoderma
?
-
-
-
-
additional information
phosphate rather than D-glyceraldehyde 3-phosphate is formed, the hydrated form of this triose is phosphorylated in position 1 to yield an unstable intermediate that decomposes to D-glyceraldehyde + phosphate
641287
Escherichia coli
?
-
-
-
-
UTP + glycerol
-
641287
Gallus gallus
UDP + glycerol 3-phosphate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 55000-57000, equilibrium ultracentrifugation in presence of 6 M guanidine HCl, SDS-PAGE
Escherichia coli
tetramer
4 * 60000, SDS-PAGE
Saccharomyces cerevisiae
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
-
Escherichia coli
additional information
-
enzyme is quite heat labile
Mycobacterium sp.
additional information
-
-
Rattus norvegicus
50
-
5 min, 30% loss of activity
Neurospora crassa
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Bacillus subtilis
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Bombus sp.
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Bos taurus
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Candida mycoderma
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Cavia porcellus
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Clostridium novyi
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Columba sp.
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Cyberlindnera jadinii
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Enterococcus faecalis
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Escherichia coli
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Felis catus
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Geotrichum candidum
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Gluconobacter oxydans
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Halobacterium salinarum
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Homo sapiens
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Klebsiella aerogenes
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Locusta sp.
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Mesocricetus auratus
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Mus musculus
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Mycobacterium butyricum
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Mycobacterium sp.
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Mycobacterium tuberculosis
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Mycolicibacterium smegmatis
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Neurospora crassa
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Nocardia asteroides
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Oryctolagus cuniculus
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Pseudomonas aeruginosa
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Rattus norvegicus
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Saccharomyces cerevisiae
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Shigella sonnei
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Staphylococcus aureus
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
trout
60
-
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods
Wickerhamomyces anomalus
70
-
-
Bacillus subtilis
70
-
-
Bombus sp.
70
-
-
Bos taurus
70
-
-
Candida mycoderma
70
-
-
Cavia porcellus
70
-
-
Clostridium novyi
70
-
-
Columba sp.
70
-
-
Cyberlindnera jadinii
70
-
-
Enterococcus faecalis
70
-
t1/2: 5 min
Escherichia coli
70
-
-
Felis catus
70
-
-
Geotrichum candidum
70
-
-
Gluconobacter oxydans
70
-
-
Halobacterium salinarum
70
-
-
Homo sapiens
70
-
-
Klebsiella aerogenes
70
-
-
Locusta sp.
70
-
-
Mesocricetus auratus
70
-
-
Mus musculus
70
-
-
Mycobacterium butyricum
70
-
-
Mycobacterium sp.
70
-
-
Mycobacterium tuberculosis
70
-
-
Mycolicibacterium smegmatis
70
-
-
Neurospora crassa
70
-
-
Nocardia asteroides
70
-
-
Oryctolagus cuniculus
70
-
-
Pseudomonas aeruginosa
70
-
-
Rattus norvegicus
70
-
-
Saccharomyces cerevisiae
70
-
-
Shigella sonnei
70
-
-
Staphylococcus aureus
70
-
-
trout
70
-
-
Wickerhamomyces anomalus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
7.5
-
Clostridium novyi
7
7.5
-
Mycobacterium sp.
8
-
-
Saccharomyces cerevisiae
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
additional information
-
glycerol affords considerable stabilization at the unfavorable pH values, glycerol kinases from microorganisms most stable at a neutral pH, glycerol kinases from higher organisms most stable in an acidic pH range
Rattus norvegicus
5
-
0°C, 6 h, without glycerol, completely stable
Columba sp.
5
-
0°C, 24 h, complete loss of activity, 30°C, 30 min, 75% loss of activity
Escherichia coli
6
9
30°C, in presence of glycerol, completely stable for 30 min
Mycobacterium sp.
7
-
0°C, 6 h, without glycerol, 90% loss of activity
Columba sp.
7
-
0°C, 24 h, 50% loss of activity
Escherichia coli
7.5
-
25°C, no loss of activity after several h
Rattus norvegicus
9.8
-
25°C, half-life: 6.5 min
Rattus norvegicus
Other publictions for EC 2.7.1.30
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738722
Fukuda
Epistasis effects of multiple ...
Cellulomonas sp., Cellulomonas sp. NT3060
J. Biosci. Bioeng.
121
497-502
2016
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8
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1
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5
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2
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3
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8
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3
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2
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1
3
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3
3
738633
Rodriguez-Contreras
Gluconeogenesis in Leishmania ...
Leishmania mexicana
J. Biol. Chem.
289
32989-33000
2014
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2
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738731
Jeong
Bioelectrocatalytic sensor for ...
Homo sapiens
J. Biotechnol.
175
7-14
2014
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738812
Park
A specific glycerol kinase ind ...
Plutella xylostella
J. Insect Physiol.
67
56-63
2014
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1
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1
-
-
739102
Balogun
Molecular basis for the revers ...
Trypanosoma brucei gambiense
Mol. Microbiol.
94
1315-1329
2014
-
-
-
1
11
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1
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2
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2
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1
11
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2
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-
-
739627
Terrazas
-
Using Pichia pastoris to produ ...
Saccharomyces cerevisiae
Rev. Cienc. Farm. Basica Apl.
35
279-284
2014
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1
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1
-
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-
-
-
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-
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-
-
-
721849
Ahmad
Enhancement of xylitol product ...
Candida tropicalis, Candida tropicalis BSXDH-3
Bioprocess Biosyst. Eng.
36
1279-1284
2013
-
-
-
-
-
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5
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2
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-
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-
1
1
-
-
-
738553
Balogun
Biochemical characterization o ...
Trypanosoma brucei gambiense
J. Biochem.
154
77-84
2013
-
-
1
-
-
-
-
2
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2
2
-
3
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1
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2
2
2
1
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1
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1
1
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1
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2
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2
2
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1
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2
2
2
1
-
1
-
-
1
1
-
-
-
-
-
-
-
-
722839
Restiawaty
Construction of membrane-ancho ...
Thermococcus kodakarensis
J. Biosci. Bioeng.
113
521-525
2012
-
-
1
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1
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5
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1
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1
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1
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1
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1
1
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1
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-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
721260
Ditlecadet
Glycerol loss to water exceeds ...
Osmerus mordax
Am. J. Physiol. Regul. Integr. Comp. Physiol.
300
R674-R684
2011
-
-
-
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3
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6
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6
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1
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-
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-
1
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-
1
-
-
722310
Aizemberg
-
Optimal conditions for biomass ...
Saccharomyces cerevisiae
Food Technol. Biotechnol.
49
329-335
2011
-
-
1
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1
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1
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1
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2
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1
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722712
Applebee
Functional and metabolic effec ...
Escherichia coli
J. Biol. Chem.
286
23150-23159
2011
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1
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4
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3
5
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1
3
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2
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1
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1
1
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1
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1
1
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4
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3
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5
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1
3
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1
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1
1
-
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-
-
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-
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-
723070
Ohashi-Suzuki
Differential kinetic activitie ...
Trypanosoma brucei brucei, Trypanosoma brucei brucei ILTat1.4, Trypanosoma congolense, Trypanosoma congolense IL1180, Trypanosoma vivax, Trypanosoma vivax IL1392
J. Vet. Med. Sci.
73
615-621
2011
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3
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3
6
3
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5
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11
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3
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12
3
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3
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3
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6
3
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5
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3
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-
12
3
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721188
Balogun
Overproduction, purification, ...
Trypanosoma brucei gambiense, Trypanosoma brucei gambiense IL2343
Acta Crystallogr. Sect. F
66
304-308
2010
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1
1
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1
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2
1
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1
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-
723091
Sriram
Moonlighting function of glyce ...
Homo sapiens
Metab. Eng.
12
332-340
2010
-
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1
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1
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2
2
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701908
Pettigrew
Amino acid substitutions in th ...
Escherichia coli
Arch. Biochem. Biophys.
481
151-156
2009
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1
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3
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1
2
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1
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701931
Pettigrew
Oligomeric interactions provid ...
Escherichia coli
Arch. Biochem. Biophys.
492
29-39
2009
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1
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4
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2
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4
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1
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702293
Yeh
Structural characterizations o ...
Enterococcus casseliflavus
Biochemistry
48
346-356
2009
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1
1
3
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3
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1
1
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1
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3
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1
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1
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-
-
-
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704024
Kihara
Glycerol kinase activity and g ...
Bombyx mori
Insect Biochem. Mol. Biol.
39
763-769
2009
-
-
-
-
-
-
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2
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3
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1
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1
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4
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1
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2
-
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2
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1
-
-
1
-
-
704298
Sherwood
Glycerol-mediated repression o ...
Haloferax volcanii
J. Bacteriol.
191
4307-4315
2009
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1
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1
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1
-
-
705388
Okawara
Bovine oocytes and early embry ...
Bos taurus
J. Reprod. Dev.
55
177-182
2009
-
-
-
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-
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-
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2
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-
-
705751
Rahib
Transcriptomic and network com ...
Mus musculus
Mol. Genet. Metab.
96
106-112
2009
-
-
-
-
-
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-
-
-
-
3
-
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-
705757
MacLennan
Weighted gene co-expression ne ...
Mus musculus
Mol. Genet. Metab.
98
203-214
2009
-
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-
4
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-
705779
Schnick
Structure and non-essential fu ...
Plasmodium falciparum
Mol. Microbiol.
71
533-545
2009
-
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-
-
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-
1
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688443
Aragon Casale
-
Characterization of glycerol k ...
Saccharomyces cerevisiae
J. Mol. Catal. B
52-53
113-120
2008
-
1
-
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-
-
1
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-
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1
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1
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1
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1
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-
-
-
705749
Sriram
Global metabolic effects of gl ...
Rattus norvegicus
Mol. Genet. Metab.
93
145-159
2008
-
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-
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-
-
-
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-
-
706466
Walmsley
Pseudo-hypertriglyceridaemia: ...
Homo sapiens
Postgrad. Med. J.
84
552-554
2008
-
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-
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-
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-
-
2
-
-
-
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-
-
-
-
677400
Katsumi
Crystallization and preliminar ...
Thermococcus kodakarensis
Acta Crystallogr. Sect. F
F63
126-129
2007
-
-
1
1
-
-
-
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-
-
1
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3
-
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-
-
678281
Anderson
Crystal structure of a hyperac ...
Escherichia coli
Biochemistry
46
5722-5731
2007
-
-
1
1
1
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5
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3
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1
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681924
Matsumura
Aquaporin 7 Is a beta-cell pro ...
Mus musculus
Mol. Cell. Biol.
27
6026-6037
2007
-
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1
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3
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-
-
-
-
684168
Katsumi
Crystallization and preliminar ...
Thermococcus kodakarensis
Acta Crystallogr. Sect. F
63
126-129
2007
-
-
-
1
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3
-
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685101
Yu
IIAGlc inhibition of glycerol ...
Escherichia coli
Biochemistry
46
12355-12365
2007
-
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4
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1
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3
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1
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-
-
-
677722
Stefuca
Development of enzyme flow cal ...
Cellulomonas sp., Escherichia coli
Appl. Microbiol. Biotechnol.
72
1170-1175
2006
-
-
-
-
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-
-
-
-
-
4
-
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-
-
-
-
681943
Martinez Agosto
Conserved family of glycerol k ...
Drosophila melanogaster
Mol. Genet. Metab.
88
334-345
2006
-
-
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-
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-
-
-
-
4
-
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-
-
-
-
-
-
-
-
660906
Ohira
Human and murine glycerol kina ...
Homo sapiens, Mus musculus
Biochem. Biophys. Res. Commun.
331
239-246
2005
-
-
2
-
-
-
-
-
6
-
-
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-
5
-
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11
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4
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11
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4
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-
-
661668
Wibmer
Novel mutation (Gly280Ala) in ...
Homo sapiens
Exp. Clin. Endocrinol. Diabetes
113
396-403
2005
-
-
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1
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-
-
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2
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677308
Vahedi-Faridi
The effects of flash-annealing ...
Enterococcus casseliflavus
Acta Crystallogr. Sect. D
61
982-989
2005
-
-
-
1
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681755
Lee
The effects of thiazolidinedio ...
Rattus norvegicus
Metab. Clin. Exp.
54
1282-1289
2005
1
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3
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-
-
-
682503
Hibuse
Aquaporin 7 deficiency is asso ...
Mus musculus
Proc. Natl. Acad. Sci. USA
102
10993-10998
2005
-
-
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1
-
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-
-
-
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3
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-
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-
661122
Yeh
Structures of enterococcal gly ...
Enterococcus casseliflavus, Escherichia coli
Biochemistry
43
362-373
2004
-
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1
2
1
-
3
-
-
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2
-
4
-
1
1
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4
3
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1
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2
1
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3
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2
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1
1
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4
3
-
-
-
-
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-
-
-
-
-
-
-
-
661655
Sjarif
Glycerol kinase deficiency: re ...
Homo sapiens
Eur. J. Hum. Genet.
12
424-432
2004
-
-
-
-
3
-
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1
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3
-
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2
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3
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1
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1
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2
-
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-
-
-
-
-
-
-
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-
-
-
641316
Yu
Linkage between fructose 1,6-b ...
Escherichia coli
Biochemistry
42
4243-4252
2003
-
-
-
-
-
-
-
-
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2
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1
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1
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-
1
-
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-
-
-
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-
-
-
-
-
-
-
-
-
641324
Sakasegawa
Stabilization of Flavobacteriu ...
Elizabethkingia meningoseptica
Biosci. Biotechnol. Biochem.
66
1374-1377
2002
-
-
-
-
2
-
-
-
-
-
-
-
-
2
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-
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2
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
641318
Sakasegawa
Increasing the thermostability ...
Elizabethkingia meningoseptica
Protein Eng.
14
663-667
2001
-
-
-
-
1
-
-
2
-
-
-
-
-
3
-
-
-
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1
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1
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2
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641321
Pawlyk
Subcloning, expression, purifi ...
Haemophilus influenzae
Protein Expr. Purif.
22
52-59
2001
-
-
1
-
-
-
2
2
-
-
1
-
-
3
-
-
1
-
-
-
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1
1
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-
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-
1
-
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2
-
2
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1
-
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1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641327
Huang
Preliminary crystallographic s ...
Thermus aquaticus
Acta Crystallogr. Sect. D
57
1030-1031
2001
-
-
-
1
-
-
-
-
-
-
-
-
-
3
-
-
-
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1
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641312
Steinborn
Cloning, heterologous expressi ...
Trypanosoma brucei
Biol. Chem.
381
1071-1077
2000
-
-
1
-
-
-
-
4
1
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
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-
-
-
2
-
-
-
-
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-
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1
-
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4
1
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-
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-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
641315
Kralova
Glycerol kinase of Trypanosoma ...
Trypanosoma brucei brucei
Eur. J. Biochem.
267
2323-2333
2000
-
-
1
-
1
-
-
4
-
-
1
-
-
9
-
-
-
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
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-
1
-
-
1
-
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-
4
-
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1
-
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-
-
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
641314
Darbon
Glycerol transport and phospho ...
Thermus thermophilus
Microbiology
145
3205-3212
1999
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641317
Mao
X-Ray structure of glycerol ki ...
Escherichia coli
Biochem. Biophys. Res. Commun.
259
640-644
1999
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
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-
-
-
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-
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-
-
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1
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-
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-
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-
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641313
Westergaard
Characterization of glycerol u ...
Rattus norvegicus
Biochim. Biophys. Acta
1402
261-268
1998
-
-
-
-
-
-
-
5
-
-
-
-
-
4
-
-
-
-
-
4
-
-
4
-
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-
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5
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4
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4
-
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-
-
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-
641319
Koga
Thermostable glycerol kinase f ...
Thermococcus kodakarensis
Protein Eng.
11
1219-1227
1998
-
-
1
-
-
-
-
2
-
2
1
-
-
5
-
-
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1
1
1
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1
-
1
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1
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2
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2
1
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-
1
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
641320
Feese
Glycerol kinase from Escherich ...
Escherichia coli
Structure
6
1407-1418
1998
-
-
-
1
2
-
-
-
-
-
-
-
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2
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1
1
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1
2
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-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641323
Pasteris
-
Characterization of glycerol k ...
Pediococcus pentosaceus
Lett. Appl. Microbiol.
27
93-97
1998
-
-
-
-
-
-
2
2
-
1
-
-
-
1
-
-
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1
-
1
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-
-
1
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2
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2
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1
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-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
641326
Ormo
Crystal structure of a complex ...
Escherichia coli
Biochemistry
37
16565-16572
1998
-
-
-
1
-
-
1
-
-
-
-
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2
-
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1
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641328
Sakasegawa
A novel glycerol kinase from F ...
Elizabethkingia meningoseptica
Biosci. Biotechnol. Biochem.
62
2388-2395
1998
-
-
1
-
-
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2
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3
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1
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1
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-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
641325
Huang
-
Purification and characterizat ...
Thermus thermophilus
J. Ferment. Bioeng.
83
328-332
1997
-
-
-
-
-
-
3
2
-
-
2
-
-
1
-
-
1
-
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1
1
1
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1
-
1
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3
-
2
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2
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1
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-
1
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
641322
Pettigrew
A single amino acid change in ...
Escherichia coli, Escherichia coli C.Lin 43
J. Bacteriol.
178
2846-2852
1996
-
-
-
-
1
-
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4
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1
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1
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-
-
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-
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-
-
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-
-
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-
641288
Krakow
Purification and characterizat ...
Trypanosoma brucei
Mol. Biochem. Parasitol.
43
17-25
1990
-
-
-
-
-
-
2
3
2
-
1
-
-
2
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1
1
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1
1
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1
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2
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3
2
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1
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1
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1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
641300
Faber
Crystallization and preliminar ...
Escherichia coli
J. Mol. Biol.
207
637-639
1989
-
-
-
1
-
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1
-
1
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2
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2
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1
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1
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1
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-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641302
Knight
Thiol and amino analogues as a ...
Candida mycoderma
Biochemistry
28
5728-5734
1989
-
-
-
-
-
-
-
3
-
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3
-
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4
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-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641307
Nilsson
-
Purification and characterizat ...
Debaryomyces hansenii
Biochim. Biophys. Acta
991
296-302
1989
-
-
-
-
-
-
6
1
-
1
1
-
-
1
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-
1
-
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-
1
-
3
-
-
-
-
-
1
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-
6
-
1
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1
1
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1
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-
1
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
641303
Kee
Improved methods for purificat ...
Escherichia coli
J. Chromatogr.
428
345-351
1988
-
-
-
-
-
-
-
-
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1
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2
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1
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1
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2
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1
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1
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1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641296
Sadava
Glycerol metabolism in higher ...
Avena sativa, Cucumis sativus, Phaseolus vulgaris, Pisum sativum, Vicia faba, Vigna radiata var. radiata, Zea mays
Biochem. Biophys. Res. Commun.
143
977-983
1987
-
-
-
-
-
-
15
4
-
2
1
-
-
10
-
-
1
-
-
13
-
-
10
-
-
-
-
-
1
7
-
-
-
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-
-
-
-
-
-
-
-
-
15
-
4
-
2
1
-
-
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1
-
13
-
-
10
-
-
-
-
-
1
7
-
-
-
-
-
-
-
-
641301
Goward
The inhibition of glucokinase ...
Geobacillus stearothermophilus
Biochem. J.
246
83-88
1987
-
-
-
-
-
-
1
1
-
-
-
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3
-
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1
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-
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1
-
1
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641298
Pettigrew
Inactivation of Escherichia co ...
Escherichia coli
Biochemistry
25
4711-4718
1986
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
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-
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1
-
-
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-
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-
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2
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-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641297
Crans
-
Glycerol kinase: substrate spe ...
Candida mycoderma, Escherichia coli, Geobacillus stearothermophilus, Saccharomyces cerevisiae
J. Am. Chem. Soc.
107
7008-7018
1985
-
-
-
-
-
-
1
-
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-
-
-
-
4
-
-
-
-
-
-
-
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8
-
-
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-
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-
-
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-
-
1
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-
-
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-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641289
Kasinathan
Phospholipid synthesizing enzy ...
Epidermophyton floccosum, Nannizzia gypsea
Lipids
19
289-293
1984
-
-
-
-
-
1
4
-
5
1
1
1
-
5
-
-
1
1
-
-
-
-
8
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
5
1
1
1
-
-
-
1
-
-
-
-
8
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
641290
Khuller
-
Glycerol kinase of Epidermophy ...
Epidermophyton floccosum
IRCS Med. Sci. Libr. Compend.
11
889-890
1983
-
-
-
-
-
-
4
2
1
2
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
2
1
2
-
-
-
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-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641291
Ramabrahmam
-
Mitochondrial glycerol kinase ...
Culex quinquefasciatus
Insect Biochem.
13
523-528
1983
-
-
-
-
-
-
4
1
1
1
-
-
-
1
-
-
1
-
-
1
-
-
3
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
1
1
1
-
-
-
-
-
1
-
1
-
-
3
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
641306
Scawen
The application of triazine dy ...
Geobacillus stearothermophilus
Anal. Biochem.
132
413-417
1983
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641309
Comer
-
Purification and properties of ...
Candida mycoderma, Escherichia coli, Geobacillus stearothermophilus
J. Appl. Biochem.
1
259-270
1979
-
-
-
-
-
-
2
15
-
-
2
-
-
3
-
-
1
-
-
-
1
-
13
2
2
1
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
15
-
-
2
-
-
-
-
1
-
-
1
-
13
2
2
1
10
-
-
-
-
-
-
-
-
-
-
-
641310
Barrera
Adipose glycerol kinase: low m ...
Gallus gallus, Mus musculus, Rattus norvegicus
Biochem. Biophys. Res. Commun.
86
145-152
1979
-
-
-
-
-
-
-
9
-
-
1
-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641292
Rao
-
Partial purification and chara ...
Gallus gallus
Int. J. Biochem.
8
295-298
1977
-
-
-
-
-
-
2
1
-
-
-
-
-
1
-
-
1
1
-
1
-
-
5
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
1
-
1
-
-
5
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
641294
Thorner
-
Glycerol kinase ...
Escherichia coli
Methods Enzymol.
42C
148-156
1975
-
-
-
1
-
-
3
2
-
4
1
-
-
1
-
-
1
-
-
-
1
1
4
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
3
-
2
-
4
1
-
-
-
-
1
-
-
1
1
4
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
641299
Schneider
Activation of bovine liver gly ...
Bos taurus
Biochim. Biophys. Acta
397
110-116
1975
18
-
-
1
-
-
6
1
-
-
-
-
-
2
-
-
1
-
-
3
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
18
-
-
-
1
-
-
-
6
-
1
-
-
-
-
-
-
-
1
-
3
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
641308
Courtright
Intracellular localization and ...
Neurospora crassa
Arch. Biochem. Biophys.
167
21-33
1975
-
-
-
-
-
1
1
-
2
-
1
-
-
2
-
-
1
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
2
-
1
-
-
-
-
1
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641293
Krause
Glycerol kinase of the porcine ...
Sus scrofa
Acta Biol. Med. Ger.
33
385-391
1974
-
-
-
-
-
-
-
1
-
1
-
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641304
Eisenthal
Specificity of glycerol kinase ...
Candida mycoderma
Biochem. J.
141
305-307
1974
2
-
-
-
-
1
9
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
1
-
9
2
3
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641305
Janson
The kinetic mechanism of glyce ...
Candida mycoderma
J. Biol. Chem.
249
2562-2566
1974
-
-
-
-
-
-
1
2
-
-
-
-
-
1
-
-
-
1
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641287
Thorner
-
Glycerol and glycerate kinases ...
Bacillus subtilis, Bombus sp., Bos taurus, Candida mycoderma, Cavia porcellus, Clostridium novyi, Columba sp., Cyberlindnera jadinii, Enterococcus faecalis, Escherichia coli, Felis catus, Gallus gallus, Geotrichum candidum, Gluconobacter oxydans, Halobacterium salinarum, Homo sapiens, Klebsiella aerogenes, Locusta sp., Mesocricetus auratus, Mus musculus, Mycobacterium butyricum, Mycobacterium sp., Mycobacterium sp. 607, Mycobacterium tuberculosis, Mycolicibacterium smegmatis, Neurospora crassa, Nocardia asteroides, Oryctolagus cuniculus, Pseudomonas aeruginosa, Rattus norvegicus, Saccharomyces cerevisiae, Shigella sonnei, Staphylococcus aureus, trout, Wickerhamomyces anomalus
The Enzymes,3rd ed. (Boyer,P. D. ,ed. )
8
487-508
1973
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4
48
7
34
1
3
2
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37
1
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2
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63
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5
50
2
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70
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3
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8
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4
-
48
-
7
34
1
3
2
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1
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63
-
5
50
2
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70
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3
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8
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641311
Thorner
Catalytic and allosteric prope ...
Escherichia coli
J. Biol. Chem.
248
3922-3932
1973
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1
1
1
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2
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1
1
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1
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1
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1
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1
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1
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1
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1
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1
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641295
Hayashi
Purification and properties of ...
Escherichia coli
J. Biol. Chem.
242
1030-1035
1967
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1
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1
2
3
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2
1
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2
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1
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1
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5
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1
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1
1
1
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1
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1
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2
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3
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2
1
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1
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1
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5
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1
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1
1
1
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641286
Bergmeyer
-
Kristallisierte Glycerokinase ...
Candida mycoderma
Biochem. Z.
333
471-480
1961
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1
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2
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1
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1
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1
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3
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2
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1
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1
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1
1
1
5
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1
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