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Literature summary for 2.7.1.30 extracted from
- Glycerol and glycerate kinases (1973), The Enzymes,3rd ed. (Boyer,P. D. ,ed. ), 8, 487-508.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| glycerol affords considerable stabilization at the unfavorable pH values | Mycobacterium sp. |
| pigeon liver enzyme is sensitive to extreme dilution but can be stabilized by addition of 0.01% bovine serum albumin | Columba sp. |
| the enzyme in crude extracts is stable to freezing and thawing, while more purified preparations are inactivated | Rattus norvegicus |
| the enzyme in crude extracts is stable to freezing and thawing, while more purified preparations are inactivated | Pseudomonas aeruginosa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Escherichia coli |  |
| ADP | - |
Gallus gallus | |
| ADP | - |
Rattus norvegicus | |
| AMP | - |
Saccharomyces cerevisiae | |
| fructose 1,6-diphosphate | not | Candida mycoderma | |
| fructose 1,6-diphosphate | - |
Escherichia coli | |
| fructose 1,6-diphosphate | not | Neurospora crassa | |
| glycerol 3-phosphate | - |
Bacillus subtilis | |
| glycerol 3-phosphate | - |
Bombus sp. | |
| glycerol 3-phosphate | - |
Bos taurus | |
| glycerol 3-phosphate | - |
Candida mycoderma | |
| glycerol 3-phosphate | - |
Cavia porcellus | |
| glycerol 3-phosphate | - |
Clostridium novyi | |
| glycerol 3-phosphate | - |
Columba sp. | |
| glycerol 3-phosphate | - |
Cyberlindnera jadinii | |
| glycerol 3-phosphate | - |
Enterococcus faecalis | |
| glycerol 3-phosphate | - |
Escherichia coli | |
| glycerol 3-phosphate | - |
Felis catus | |
| glycerol 3-phosphate | - |
Geotrichum candidum | |
| glycerol 3-phosphate | - |
Gluconobacter oxydans | |
| glycerol 3-phosphate | - |
Halobacterium salinarum | |
| glycerol 3-phosphate | - |
Homo sapiens | |
| glycerol 3-phosphate | - |
Klebsiella aerogenes | |
| glycerol 3-phosphate | - |
Locusta sp. | |
| glycerol 3-phosphate | - |
Mesocricetus auratus | |
| glycerol 3-phosphate | - |
Mus musculus | |
| glycerol 3-phosphate | - |
Mycobacterium butyricum | |
| glycerol 3-phosphate | - |
Mycobacterium sp. | |
| glycerol 3-phosphate | - |
Mycobacterium tuberculosis | |
| glycerol 3-phosphate | - |
Mycolicibacterium smegmatis | |
| glycerol 3-phosphate | - |
Neurospora crassa | |
| glycerol 3-phosphate | - |
Nocardia asteroides | |
| glycerol 3-phosphate | - |
Oryctolagus cuniculus | |
| glycerol 3-phosphate | - |
Pseudomonas aeruginosa | |
| glycerol 3-phosphate | competitive to glycerol | Rattus norvegicus | |
| glycerol 3-phosphate | - |
Saccharomyces cerevisiae | |
| glycerol 3-phosphate | - |
Shigella sonnei | |
| glycerol 3-phosphate | - |
Staphylococcus aureus | |
| glycerol 3-phosphate | - |
trout | |
| glycerol 3-phosphate | - |
Wickerhamomyces anomalus | |
| iodoacetamide | - |
Candida mycoderma | |
| iodoacetamide | - |
Rattus norvegicus | |
| iodoacetate | - |
Escherichia coli | |
| N-ethylmaleimide | - |
Escherichia coli | |
| N-ethylmaleimide | - |
Rattus norvegicus | |
| p-chloromercuribenzoate | - |
Candida mycoderma | |
| p-chloromercuribenzoate | - |
Rattus norvegicus | |
| p-Hydroxymercuriphenylsulfonate | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Gallus gallus | |
| additional information | - |
additional information | - |
Mus musculus | |
| additional information | - |
additional information | - |
Escherichia coli | |
| additional information | - |
additional information | - |
Rattus norvegicus | |
| additional information | - |
additional information | - |
Bos taurus | |
| additional information | - |
additional information | - |
Columba sp. | |
| additional information | - |
additional information | - |
Candida mycoderma |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | described in the most tissues | Cavia porcellus | 5829 | - |
| cytosol | described in the most tissues | Staphylococcus aureus | 5829 | - |
| cytosol | described in the most tissues | Bacillus subtilis | 5829 | - |
| cytosol | described in the most tissues | Cyberlindnera jadinii | 5829 | - |
| cytosol | described in the most tissues | Mus musculus | 5829 | - |
| cytosol | described in the most tissues | Escherichia coli | 5829 | - |
| cytosol | described in the most tissues | Homo sapiens | 5829 | - |
| cytosol | described in the most tissues | Rattus norvegicus | 5829 | - |
| cytosol | described in the most tissues | Saccharomyces cerevisiae | 5829 | - |
| cytosol | described in the most tissues | Bos taurus | 5829 | - |
| cytosol | described in the most tissues | Oryctolagus cuniculus | 5829 | - |
| cytosol | described in the most tissues | Mycolicibacterium smegmatis | 5829 | - |
| cytosol | described in the most tissues | Neurospora crassa | 5829 | - |
| cytosol | described in the most tissues | Klebsiella aerogenes | 5829 | - |
| cytosol | described in the most tissues | Pseudomonas aeruginosa | 5829 | - |
| cytosol | described in the most tissues | Halobacterium salinarum | 5829 | - |
| cytosol | described in the most tissues | Enterococcus faecalis | 5829 | - |
| cytosol | described in the most tissues | Mycobacterium tuberculosis | 5829 | - |
| cytosol | described in the most tissues | Mesocricetus auratus | 5829 | - |
| cytosol | described in the most tissues | Felis catus | 5829 | - |
| cytosol | described in the most tissues | Mycobacterium sp. | 5829 | - |
| cytosol | described in the most tissues | Nocardia asteroides | 5829 | - |
| cytosol | described in the most tissues | Gluconobacter oxydans | 5829 | - |
| cytosol | described in the most tissues | Mycobacterium butyricum | 5829 | - |
| cytosol | described in the most tissues | Wickerhamomyces anomalus | 5829 | - |
| cytosol | described in the most tissues | Shigella sonnei | 5829 | - |
| cytosol | described in the most tissues | trout | 5829 | - |
| cytosol | described in the most tissues | Geotrichum candidum | 5829 | - |
| cytosol | described in the most tissues | Columba sp. | 5829 | - |
| cytosol | described in the most tissues | Locusta sp. | 5829 | - |
| cytosol | described in the most tissues | Bombus sp. | 5829 | - |
| cytosol | described in the most tissues | Candida mycoderma | 5829 | - |
| cytosol | described in the most tissues | Clostridium novyi | 5829 | - |
| mitochondrion | - |
Bos taurus | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
4 * 60000, SDS-PAGE | Saccharomyces cerevisiae |
| 140000 | - |
gel filtration, zone sedimentation in sucrose density gradient | Neurospora crassa |
| 210000 | 217000 | equilibrium sedimentation | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + glycerol | Escherichia coli | enzyme functions primarily in the utilization of glycerol as a carbon and energy source | ? | - |
? | |
| ATP + glycerol | Mesocricetus auratus | higher organisms: salvage of glycerol released upon lipolysis | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
| Bombus sp. | - |
queen bumblebee | - |
| Bos taurus | - |
- |
- |
| Candida mycoderma | - |
- |
- |
| Cavia porcellus | - |
- |
- |
| Clostridium novyi | - |
- |
- |
| Columba sp. | - |
pigeon | - |
| Cyberlindnera jadinii | - |
- |
- |
| Enterococcus faecalis | - |
- |
- |
| Escherichia coli | - |
- |
- |
| Felis catus | - |
- |
- |
| Gallus gallus | - |
- |
- |
| Geotrichum candidum | - |
- |
- |
| Gluconobacter oxydans | - |
- |
- |
| Halobacterium salinarum | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Klebsiella aerogenes | - |
- |
- |
| Locusta sp. | - |
- |
- |
| Mesocricetus auratus | - |
- |
- |
| Mus musculus | - |
- |
- |
| Mycobacterium butyricum | - |
- |
- |
| Mycobacterium sp. | - |
607 | - |
| Mycobacterium sp. 607 | - |
607 | - |
| Mycobacterium tuberculosis | - |
- |
- |
| Mycolicibacterium smegmatis | - |
- |
- |
| Neurospora crassa | - |
- |
- |
| Nocardia asteroides | - |
- |
- |
| Oryctolagus cuniculus | - |
- |
- |
| Pseudomonas aeruginosa | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
| Saccharomyces cerevisiae | - |
- |
- |
| Shigella sonnei | - |
- |
- |
| Staphylococcus aureus | - |
- |
- |
| trout | - |
- |
- |
| Wickerhamomyces anomalus | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| susceptible to inactivation by oxidation of sulfhydryl groups | Columba sp. |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + glycerol = ADP + sn-glycerol 3-phosphate | ordered mechanism | Escherichia coli | |
| ATP + glycerol = ADP + sn-glycerol 3-phosphate | ordered mechanism | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adipose tissue | - |
Gallus gallus | - |
| adipose tissue | - |
Bos taurus | - |
| adipose tissue | white and brown | Mus musculus | - |
| adipose tissue | white and brown | Homo sapiens | - |
| adipose tissue | white and brown | Rattus norvegicus | - |
| adipose tissue | white and brown | Oryctolagus cuniculus | - |
| adipose tissue | white and brown | Locusta sp. | - |
| intestine | - |
Rattus norvegicus | - |
| intestine | - |
Mesocricetus auratus | - |
| intestine | - |
Felis catus | - |
| kidney | - |
Rattus norvegicus | - |
| liver | - |
Gallus gallus | - |
| liver | - |
Mus musculus | - |
| liver | - |
Rattus norvegicus | - |
| liver | - |
Bos taurus | - |
| liver | - |
Mesocricetus auratus | - |
| liver | - |
trout | - |
| liver | - |
Columba sp. | - |
| additional information | enzyme activity in various tissues | Cavia porcellus | - |
| additional information | enzyme activity in various tissues | Staphylococcus aureus | - |
| additional information | enzyme activity in various tissues | Bacillus subtilis | - |
| additional information | enzyme activity in various tissues | Cyberlindnera jadinii | - |
| additional information | enzyme activity in various tissues | Mus musculus | - |
| additional information | enzyme activity in various tissues | Escherichia coli | - |
| additional information | enzyme activity in various tissues | Homo sapiens | - |
| additional information | enzyme activity in various tissues | Rattus norvegicus | - |
| additional information | enzyme activity in various tissues | Saccharomyces cerevisiae | - |
| additional information | enzyme activity in various tissues | Bos taurus | - |
| additional information | enzyme activity in various tissues | Oryctolagus cuniculus | - |
| additional information | enzyme activity in various tissues | Mycolicibacterium smegmatis | - |
| additional information | enzyme activity in various tissues | Neurospora crassa | - |
| additional information | enzyme activity in various tissues | Klebsiella aerogenes | - |
| additional information | enzyme activity in various tissues | Pseudomonas aeruginosa | - |
| additional information | enzyme activity in various tissues | Halobacterium salinarum | - |
| additional information | enzyme activity in various tissues | Enterococcus faecalis | - |
| additional information | enzyme activity in various tissues | Mycobacterium tuberculosis | - |
| additional information | enzyme activity in various tissues | Mesocricetus auratus | - |
| additional information | enzyme activity in various tissues | Felis catus | - |
| additional information | enzyme activity in various tissues | Mycobacterium sp. | - |
| additional information | enzyme activity in various tissues | Nocardia asteroides | - |
| additional information | enzyme activity in various tissues | Gluconobacter oxydans | - |
| additional information | enzyme activity in various tissues | Mycobacterium butyricum | - |
| additional information | enzyme activity in various tissues | Wickerhamomyces anomalus | - |
| additional information | enzyme activity in various tissues | Shigella sonnei | - |
| additional information | enzyme activity in various tissues | trout | - |
| additional information | enzyme activity in various tissues | Geotrichum candidum | - |
| additional information | enzyme activity in various tissues | Columba sp. | - |
| additional information | enzyme activity in various tissues | Locusta sp. | - |
| additional information | enzyme activity in various tissues | Bombus sp. | - |
| additional information | enzyme activity in various tissues | Candida mycoderma | - |
| additional information | enzyme activity in various tissues | Clostridium novyi | - |
| muscle | - |
Gallus gallus | - |
| muscle | - |
Rattus norvegicus | - |
| muscle | - |
Oryctolagus cuniculus | - |
| muscle | - |
trout | - |
| muscle | - |
Columba sp. | - |
| muscle | - |
Locusta sp. | - |
| muscle | - |
Bombus sp. | - |
| spermatozoon | - |
Gallus gallus | - |
| spermatozoon | - |
Homo sapiens | - |
| spermatozoon | - |
Bos taurus | - |
| spermatozoon | - |
Oryctolagus cuniculus | - |
| spermatozoon | - |
trout | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, pH 5.0, partially purified enzymes have half-lives of several weeks to months | Rattus norvegicus |
| -20°C, pH 5.0, partially purified enzymes have half-lives of several weeks to months | Mesocricetus auratus |
| -20°C, pH 5.0, partially purified enzymes have half-lives of several weeks to months | Columba sp. |
| as crystalline suspension in saturated ammonium sulfate, solutions containing 10 mM glycerol, 1 mM EDTA and a thiol e.g. 2-mercaptoethanol, yeast enzyme stable for several months, E. coli enzyme for several years | Escherichia coli |
| as crystalline suspension in saturated ammonium sulfate, solutions containing 10 mM glycerol, 1 mM EDTA and a thiol e.g. 2-mercaptoethanol, yeast enzyme stable for several months, E. coli enzyme for several years | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-glyceraldehyde | - |
Escherichia coli | ADP + D-glyceraldehyde 3-phosphate | - |
? | |
| ATP + dihydroxyacetone | - |
Escherichia coli | ADP + dihydroxyacetone phosphate | - |
? | |
| ATP + dihydroxyacetone | - |
Candida mycoderma | ADP + dihydroxyacetone phosphate | - |
? | |
| ATP + glycerol | - |
Gallus gallus | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + glycerol | enzyme can utilize only ATP as phosphoryl group donor | Cavia porcellus | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + glycerol | enzyme can utilize only ATP as phosphoryl group donor | Staphylococcus aureus | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + glycerol | enzyme can utilize only ATP as phosphoryl group donor | Bacillus subtilis | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + glycerol | enzyme can utilize only ATP as phosphoryl group donor | Cyberlindnera jadinii | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + glycerol | enzyme can utilize only ATP as phosphoryl group donor | Mus musculus | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + glycerol | enzyme can utilize only ATP as phosphoryl group donor | Escherichia coli | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + glycerol | enzyme can utilize only ATP as phosphoryl group donor | Homo sapiens | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + glycerol | enzyme can utilize only ATP as phosphoryl group donor | Rattus norvegicus | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + glycerol | enzyme can utilize only ATP as phosphoryl group donor | Saccharomyces cerevisiae | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + glycerol | enzyme can utilize only ATP as phosphoryl group donor | Bos taurus | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + glycerol | enzyme can utilize only ATP as phosphoryl group donor | Oryctolagus cuniculus | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + glycerol | enzyme can utilize only ATP as phosphoryl group donor | Mycolicibacterium smegmatis | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + glycerol | enzyme can utilize only ATP as phosphoryl group donor | Neurospora crassa | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + glycerol | enzyme can utilize only ATP as phosphoryl group donor | Klebsiella aerogenes | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + glycerol | enzyme can utilize only ATP as phosphoryl group donor | Pseudomonas aeruginosa | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + glycerol | enzyme can utilize only ATP as phosphoryl group donor | Halobacterium salinarum | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + glycerol | enzyme can utilize only ATP as phosphoryl group donor | Enterococcus faecalis | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + glycerol | enzyme can utilize only ATP as phosphoryl group donor | Mycobacterium tuberculosis | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + glycerol | enzyme can utilize only ATP as phosphoryl group donor | Mesocricetus auratus | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + glycerol | enzyme can utilize only ATP as phosphoryl group donor | Felis catus | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + glycerol | enzyme can utilize only ATP as phosphoryl group donor | Mycobacterium sp. | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + glycerol | enzyme can utilize only ATP as phosphoryl group donor | Nocardia asteroides | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + glycerol | enzyme can utilize only ATP as phosphoryl group donor | Gluconobacter oxydans | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + glycerol | enzyme can utilize only ATP as phosphoryl group donor | Mycobacterium butyricum | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + glycerol | enzyme can utilize only ATP as phosphoryl group donor | Wickerhamomyces anomalus | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + glycerol | enzyme can utilize only ATP as phosphoryl group donor | Shigella sonnei | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + glycerol | enzyme can utilize only ATP as phosphoryl group donor | trout | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + glycerol | enzyme can utilize only ATP as phosphoryl group donor | Geotrichum candidum | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + glycerol | enzyme can utilize only ATP as phosphoryl group donor | Columba sp. | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + glycerol | enzyme can utilize only ATP as phosphoryl group donor | Locusta sp. | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + glycerol | enzyme can utilize only ATP as phosphoryl group donor | Bombus sp. | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + glycerol | enzyme can utilize only ATP as phosphoryl group donor | Candida mycoderma | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + glycerol | enzyme can utilize only ATP as phosphoryl group donor | Clostridium novyi | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + glycerol | enzyme can utilize only ATP as phosphoryl group donor | Mycobacterium sp. 607 | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + glycerol | enzyme functions primarily in the utilization of glycerol as a carbon and energy source | Escherichia coli | ? | - |
? | |
| ATP + glycerol | higher organisms: salvage of glycerol released upon lipolysis | Mesocricetus auratus | ? | - |
? | |
| ATP + L-glyceraldehyde | - |
Escherichia coli | ADP + L-glyceraldehyde 3-phosphate | - |
? | |
| ATP + L-glyceraldehyde | - |
Candida mycoderma | ADP + L-glyceraldehyde 3-phosphate | - |
? | |
| CTP + glycerol | - |
Gallus gallus | CDP + glycerol 3-phosphate | - |
? | |
| CTP + glycerol | - |
Candida mycoderma | CDP + glycerol 3-phosphate | - |
? | |
| ITP + glycerol | - |
Gallus gallus | IDP + glycerol 3-phosphate | - |
? | |
| ITP + glycerol | - |
Candida mycoderma | IDP + glycerol 3-phosphate | - |
? | |
| additional information | - |
Saccharomyces cerevisiae | ? | - |
? | |
| additional information | - |
Candida mycoderma | ? | - |
? | |
| additional information | phosphate rather than D-glyceraldehyde 3-phosphate is formed, the hydrated form of this triose is phosphorylated in position 1 to yield an unstable intermediate that decomposes to D-glyceraldehyde + phosphate | Escherichia coli | ? | - |
? | |
| UTP + glycerol | - |
Gallus gallus | UDP + glycerol 3-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 60000, SDS-PAGE | Saccharomyces cerevisiae |
| tetramer | 4 * 55000-57000, equilibrium ultracentrifugation in presence of 6 M guanidine HCl, SDS-PAGE | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
| additional information | - |
- |
Rattus norvegicus |
| additional information | - |
enzyme is quite heat labile | Mycobacterium sp. |
| 50 | - |
5 min, 30% loss of activity | Neurospora crassa |
| 60 | - |
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods | Cavia porcellus |
| 60 | - |
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods | Staphylococcus aureus |
| 60 | - |
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods | Bacillus subtilis |
| 60 | - |
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods | Cyberlindnera jadinii |
| 60 | - |
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods | Mus musculus |
| 60 | - |
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods | Escherichia coli |
| 60 | - |
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods | Homo sapiens |
| 60 | - |
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods | Rattus norvegicus |
| 60 | - |
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods | Saccharomyces cerevisiae |
| 60 | - |
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods | Bos taurus |
| 60 | - |
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods | Oryctolagus cuniculus |
| 60 | - |
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods | Mycolicibacterium smegmatis |
| 60 | - |
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods | Neurospora crassa |
| 60 | - |
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods | Klebsiella aerogenes |
| 60 | - |
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods | Pseudomonas aeruginosa |
| 60 | - |
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods | Halobacterium salinarum |
| 60 | - |
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods | Enterococcus faecalis |
| 60 | - |
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods | Mycobacterium tuberculosis |
| 60 | - |
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods | Mesocricetus auratus |
| 60 | - |
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods | Felis catus |
| 60 | - |
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods | Mycobacterium sp. |
| 60 | - |
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods | Nocardia asteroides |
| 60 | - |
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods | Gluconobacter oxydans |
| 60 | - |
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods | Mycobacterium butyricum |
| 60 | - |
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods | Wickerhamomyces anomalus |
| 60 | - |
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods | Shigella sonnei |
| 60 | - |
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods | trout |
| 60 | - |
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods | Geotrichum candidum |
| 60 | - |
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods | Columba sp. |
| 60 | - |
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods | Locusta sp. |
| 60 | - |
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods | Bombus sp. |
| 60 | - |
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods | Candida mycoderma |
| 60 | - |
in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods | Clostridium novyi |
| 70 | - |
- |
Cavia porcellus |
| 70 | - |
- |
Staphylococcus aureus |
| 70 | - |
- |
Bacillus subtilis |
| 70 | - |
- |
Cyberlindnera jadinii |
| 70 | - |
- |
Mus musculus |
| 70 | - |
- |
Homo sapiens |
| 70 | - |
- |
Rattus norvegicus |
| 70 | - |
- |
Saccharomyces cerevisiae |
| 70 | - |
- |
Bos taurus |
| 70 | - |
- |
Oryctolagus cuniculus |
| 70 | - |
- |
Mycolicibacterium smegmatis |
| 70 | - |
- |
Neurospora crassa |
| 70 | - |
- |
Klebsiella aerogenes |
| 70 | - |
- |
Pseudomonas aeruginosa |
| 70 | - |
- |
Halobacterium salinarum |
| 70 | - |
- |
Enterococcus faecalis |
| 70 | - |
- |
Mycobacterium tuberculosis |
| 70 | - |
- |
Mesocricetus auratus |
| 70 | - |
- |
Felis catus |
| 70 | - |
- |
Mycobacterium sp. |
| 70 | - |
- |
Nocardia asteroides |
| 70 | - |
- |
Gluconobacter oxydans |
| 70 | - |
- |
Mycobacterium butyricum |
| 70 | - |
- |
Wickerhamomyces anomalus |
| 70 | - |
- |
Shigella sonnei |
| 70 | - |
- |
trout |
| 70 | - |
- |
Geotrichum candidum |
| 70 | - |
- |
Columba sp. |
| 70 | - |
- |
Locusta sp. |
| 70 | - |
- |
Bombus sp. |
| 70 | - |
- |
Candida mycoderma |
| 70 | - |
- |
Clostridium novyi |
| 70 | - |
t1/2: 5 min | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | - |
Mycobacterium sp. |
| 7 | 7.5 | - |
Clostridium novyi |
| 8 | - |
- |
Saccharomyces cerevisiae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
glycerol affords considerable stabilization at the unfavorable pH values, glycerol kinases from microorganisms most stable at a neutral pH, glycerol kinases from higher organisms most stable in an acidic pH range | Rattus norvegicus |
| 5 | - |
0°C, 24 h, complete loss of activity, 30°C, 30 min, 75% loss of activity | Escherichia coli |
| 5 | - |
0°C, 6 h, without glycerol, completely stable | Columba sp. |
| 6 | 9 | 30°C, in presence of glycerol, completely stable for 30 min | Mycobacterium sp. |
| 7 | - |
0°C, 6 h, without glycerol, 90% loss of activity | Columba sp. |
| 7 | - |
0°C, 24 h, 50% loss of activity | Escherichia coli |
| 7.5 | - |
25°C, no loss of activity after several h | Rattus norvegicus |
| 9.8 | - |
25°C, half-life: 6.5 min | Rattus norvegicus |
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Release 2023.1 (January 2023)
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