Crystallization (Comment) | Organism |
---|---|
DhaK-DhaL complex bound to ADP anddihydroxyacetone, hanging drop vapor diffusion method, using 0.1 M HEPES pH 7.5, 3.5 M sodium formate or 0.1 M sodium citrate pH 5.6, 20% (w/v)PEG 8000, at 19°C | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D109A | inactive | Escherichia coli |
D109N | inactive | Escherichia coli |
H128A | inactive | Escherichia coli |
H128K | inactive | Escherichia coli |
H56A | the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme | Escherichia coli |
H56N | the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
dihydroxyacetone | apparent value, Km above 0.008 mM, wild type enzyme, in 50 mM potassium phosphate pH 7.5, at 22°C | Escherichia coli | |
0.302 | - |
dihydroxyacetone | apparent value, mutant enzyme H56A, in 50 mM potassium phosphate pH 7.5, at 22°C | Escherichia coli | |
2.2 | - |
dihydroxyacetone | apparent value, mutant enzyme H56N, in 50 mM potassium phosphate pH 7.5, at 22°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P76015 | subunit DhaK | - |
Purification (Comment) | Organism |
---|---|
nickel affinity column chromatography and Superdex 200 gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + dihydroxyacetone | - |
Escherichia coli | ADP + dihydroxyacetone phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | - |
Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
DHA kinase | - |
Escherichia coli |
DhaK | subunit | Escherichia coli |
DhaL | subunit | Escherichia coli |
dihydroxyacetone kinase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
dihydroxyacetone | apparent value, mutant enzyme H56A, in 50 mM potassium phosphate pH 7.5, at 22°C | Escherichia coli | |
3.72 | - |
dihydroxyacetone | apparent value, mutant enzyme H56N, in 50 mM potassium phosphate pH 7.5, at 22°C | Escherichia coli | |
8.45 | - |
dihydroxyacetone | apparent value, Km above 0.008 mM, wild type enzyme, in 50 mM potassium phosphate pH 7.5, at 22°C | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.7 | - |
dihydroxyacetone | apparent value, mutant enzyme H56N, in 50 mM potassium phosphate pH 7.5, at 22°C | Escherichia coli | |
5.3 | - |
dihydroxyacetone | apparent value, mutant enzyme H56A, in 50 mM potassium phosphate pH 7.5, at 22°C | Escherichia coli | |
954 | - |
dihydroxyacetone | apparent value, Km above 0.008 mM, wild type enzyme, in 50 mM potassium phosphate pH 7.5, at 22°C | Escherichia coli |