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Literature summary for 2.7.1.29 extracted from

  • Shi, R.; McDonald, L.; Cui, Q.; Matte, A.; Cygler, M.; Ekiel, I.
    Structural and mechanistic insight into covalent substrate binding by Escherichia coli dihydroxyacetone kinase (2011), Proc. Natl. Acad. Sci. USA, 108, 1302-1307.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
DhaK-DhaL complex bound to ADP anddihydroxyacetone, hanging drop vapor diffusion method, using 0.1 M HEPES pH 7.5, 3.5 M sodium formate or 0.1 M sodium citrate pH 5.6, 20% (w/v)PEG 8000, at 19°C Escherichia coli

Protein Variants

Protein Variants Comment Organism
D109A inactive Escherichia coli
D109N inactive Escherichia coli
H128A inactive Escherichia coli
H128K inactive Escherichia coli
H56A the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme Escherichia coli
H56N the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
dihydroxyacetone apparent value, Km above 0.008 mM, wild type enzyme, in 50 mM potassium phosphate pH 7.5, at 22°C Escherichia coli
0.302
-
dihydroxyacetone apparent value, mutant enzyme H56A, in 50 mM potassium phosphate pH 7.5, at 22°C Escherichia coli
2.2
-
dihydroxyacetone apparent value, mutant enzyme H56N, in 50 mM potassium phosphate pH 7.5, at 22°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P76015 subunit DhaK
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography and Superdex 200 gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + dihydroxyacetone
-
Escherichia coli ADP + dihydroxyacetone phosphate
-
?

Subunits

Subunits Comment Organism
heterotetramer
-
Escherichia coli

Synonyms

Synonyms Comment Organism
DHA kinase
-
Escherichia coli
DhaK subunit Escherichia coli
DhaL subunit Escherichia coli
dihydroxyacetone kinase
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6
-
dihydroxyacetone apparent value, mutant enzyme H56A, in 50 mM potassium phosphate pH 7.5, at 22°C Escherichia coli
3.72
-
dihydroxyacetone apparent value, mutant enzyme H56N, in 50 mM potassium phosphate pH 7.5, at 22°C Escherichia coli
8.45
-
dihydroxyacetone apparent value, Km above 0.008 mM, wild type enzyme, in 50 mM potassium phosphate pH 7.5, at 22°C Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.7
-
dihydroxyacetone apparent value, mutant enzyme H56N, in 50 mM potassium phosphate pH 7.5, at 22°C Escherichia coli
5.3
-
dihydroxyacetone apparent value, mutant enzyme H56A, in 50 mM potassium phosphate pH 7.5, at 22°C Escherichia coli
954
-
dihydroxyacetone apparent value, Km above 0.008 mM, wild type enzyme, in 50 mM potassium phosphate pH 7.5, at 22°C Escherichia coli