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Literature summary for 2.7.1.29 extracted from

  • Iturrate, L.; Sanchez-Moreno, I.; Oroz-Guinea, I.; Perez-Gil, J.; Garcia-Junceda, E.
    Preparation and characterization of a bifunctional aldolase/kinase enzyme: a more efficient biocatalyst for C-C bond formation (2010), Chemistry, 16, 4018-4030.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Citrobacter freundii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00122
-
dihydroxyacetone pH and temperature not specified in the publication Citrobacter freundii

Organism

Organism UniProt Comment Textmining
Citrobacter freundii
-
-
-
Citrobacter freundii CECT 4626
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni+2/IDA agarose column chromatography and Superdex 75 gel filtration Citrobacter freundii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + dihydroxyacetone
-
Citrobacter freundii ADP + dihydroxyacetone phosphate
-
?
ATP + dihydroxyacetone
-
Citrobacter freundii CECT 4626 ADP + dihydroxyacetone phosphate
-
?

Subunits

Subunits Comment Organism
dimer
-
Citrobacter freundii

Synonyms

Synonyms Comment Organism
DhaK
-
Citrobacter freundii
dihydroxyacetone kinase
-
Citrobacter freundii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
48.7
-
the melting temperature is 48.7°C Citrobacter freundii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
24.13
-
dihydroxyacetone pH and temperature not specified in the publication Citrobacter freundii

Cofactor

Cofactor Comment Organism Structure
ATP dependent on Citrobacter freundii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
19800
-
dihydroxyacetone pH and temperature not specified in the publication Citrobacter freundii