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Literature summary for 2.7.1.29 extracted from

  • Gutknecht, R.; Beutler, R.; Garcia-Alles, L.F.; Baumann, U.; Erni, B.
    The dihydroxyacetone kinase of Escherichia coli utilizes a phosphoprotein instead of ATP as phosphoryl donor (2001), EMBO J., 20, 2480-2486.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Escherichia coli

Protein Variants

Protein Variants Comment Organism
H169A completely inactive Escherichia coli
H439A completely inactive Escherichia coli
H9A completely inactive Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.045
-
dihydroxyacetone pH 7.5, 30°C, DhaK-DhaL-DhaM complex Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
2 * 52000 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P37349 phosphoryl donor DhaM
-
Escherichia coli P76014 DhaL subunit
-
Escherichia coli P76015 DhaK subunit
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose, Superdex 75 Escherichia coli
HighQ anion exchange, Superdex 200 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphorylated DhaM domain of dihydroxyacetone kinase + dihydroxyacetone enzyme complex uses the PEP:sugar phosphotransferase protein DhaM instead of ATP as phosphoryl donor Escherichia coli dephospho-DhaM + dihydroxyacetone phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 52000 Escherichia coli
More approx. 18000 Da, monomeric state is suggested Escherichia coli
More approx. 35000 Da, monomeric state is suggested Escherichia coli
More enzyme comlex is present in an approx. 1/1/1 ratio of DhaK, DhaL and DhaM Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8
-
dihydroxyacetone pH 7.5, 30°C, DhaK-DhaL-DhaM complex Escherichia coli