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Literature summary for 2.7.1.29 extracted from
- Glycerone kinase from Candida methylica (1990), Methods Enzymol., 188, 451-455.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | 5 mM, 60% inhibition | [Candida] methylica |  |
| Mn2+ | MnCl2 | [Candida] methylica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.011 | - |
dihydroxyacetone | pH 7.5, 30°C | [Candida] methylica | |
| 0.024 | - |
DL-glyceraldehyde | pH 7.5, 30°C | [Candida] methylica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | can partially replace Mg2+ in activation, 30.3% of activity with Mg2+ | [Candida] methylica | |
| Co2+ | can partially replace Mg2+ in activation, 57.3% of activity with Mg2+ | [Candida] methylica | |
| Mg2+ | divalent cation required, highest activity with Mg2+ | [Candida] methylica | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + dihydroxyacetone | [Candida] methylica | second step in assimilation of formaldehyde via the xylulose monophosphate, i.e. dihydroxyacetone cycle during growth of yeast on methanol | ADP + dihydroxyacetone phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ogataea angusta | - |
- |
- |
| Ogataea angusta CBS 4732 | - |
- |
- |
| [Candida] methylica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ogataea angusta |
| streptomycin sulfate, ammonium sulfate, Cibacron blue F3G-ASephadex g-200, DEAE cellulose, partially purified | [Candida] methylica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 37.65 | - |
- |
[Candida] methylica |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 60% glycerol, at least 4 weeks, no loss of activity | [Candida] methylica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + dihydroxyacetone | - |
Ogataea angusta | ADP + dihydroxyacetone phosphate | - |
? | |
| ATP + dihydroxyacetone | - |
[Candida] methylica | ADP + dihydroxyacetone phosphate | - |
? | |
| ATP + dihydroxyacetone | second step in assimilation of formaldehyde via the xylulose monophosphate, i.e. dihydroxyacetone cycle during growth of yeast on methanol | [Candida] methylica | ADP + dihydroxyacetone phosphate | - |
? | |
| ATP + dihydroxyacetone | - |
Ogataea angusta CBS 4732 | ADP + dihydroxyacetone phosphate | - |
? | |
| ATP + DL-glyceraldehyde | 25% of the activity with dihydroxyacetone | [Candida] methylica | ADP + glyceraldehyde 3-phosphate | - |
? | |
| CTP + dihydroxyacetone | lower than 1% of the activity with ATP | [Candida] methylica | CDP + dihydroxyacetone phosphate | - |
? | |
| GTP + dihydroxyacetone | lower than 1% of the activity with ATP | [Candida] methylica | GDP + dihydroxyacetone phosphate | - |
? | |
| ITP + dihydroxyacetone | 11.2% of activity with ATP | [Candida] methylica | IDP + dihydroxyacetone phosphate | - |
? | |
| UTP + dihydroxyacetone | 3.1% of the activity with ATP | [Candida] methylica | UDP + dihydroxyacetone phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | [Candida] methylica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
[Candida] methylica |
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