Protein Variants | Comment | Organism |
---|---|---|
C404A | the mutant shows reduced activity compared to the wild type enzyme | Homo sapiens |
H221A | inactive | Homo sapiens |
K204A | the mutant shows reduced activity compared to the wild type enzyme | Homo sapiens |
S446A | the mutant shows reduced activity compared to the wild type enzyme | Homo sapiens |
T112A | the mutant shows reduced activity compared to the wild type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00574 | - |
ATP | mutant enzyme S446A, with dihydroxyacetone as cosubstrate, at pH 7.5 and 37°C | Homo sapiens | |
0.0071 | - |
D-glyceraldehyde | mutant enzyme S446A, at pH 7.5 and 37°C | Homo sapiens | |
0.0099 | - |
dihydroxyacetone | mutant enzyme S446A, at pH 7.5 and 37°C | Homo sapiens | |
0.0137 | - |
D-glyceraldehyde | mutant enzyme T112A, at pH 7.5 and 37°C | Homo sapiens | |
0.015 | - |
D-glyceraldehyde | mutant enzyme K204A, at pH 7.5 and 37°C | Homo sapiens | |
0.0155 | - |
dihydroxyacetone | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens | |
0.0162 | - |
D-glyceraldehyde | mutant enzyme C404A, at pH 7.5 and 37°C | Homo sapiens | |
0.0181 | - |
D-glyceraldehyde | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens | |
0.0191 | - |
dihydroxyacetone | mutant enzyme C404A, at pH 7.5 and 37°C | Homo sapiens | |
0.0252 | - |
dihydroxyacetone | mutant enzyme K204A, at pH 7.5 and 37°C | Homo sapiens | |
0.0352 | - |
ATP | mutant enzyme S446A, with D-glyceraldehyde as cosubstrate, at pH 7.5 and 37°C | Homo sapiens | |
0.0432 | - |
ATP | wild type enzyme, with dihydroxyacetone as cosubstrate, at pH 7.5 and 37°C | Homo sapiens | |
0.0624 | - |
ATP | wild type enzyme, with D-glyceraldehyde as cosubstrate, at pH 7.5 and 37°C | Homo sapiens | |
0.0637 | - |
ATP | mutant enzyme K204A, with dihydroxyacetone as cosubstrate, at pH 7.5 and 37°C | Homo sapiens | |
0.0663 | - |
dihydroxyacetone | mutant enzyme T112A, at pH 7.5 and 37°C | Homo sapiens | |
0.0696 | - |
ATP | mutant enzyme K204A, with D-glyceraldehyde as cosubstrate, at pH 7.5 and 37°C | Homo sapiens | |
0.0806 | - |
ATP | mutant enzyme T112A, with dihydroxyacetone as cosubstrate, at pH 7.5 and 37°C | Homo sapiens | |
0.0853 | - |
ATP | mutant enzyme T112A, with D-glyceraldehyde as cosubstrate, at pH 7.5 and 37°C | Homo sapiens | |
0.0996 | - |
ATP | mutant enzyme C404A, with dihydroxyacetone as cosubstrate, at pH 7.5 and 37°C | Homo sapiens | |
0.183 | - |
ATP | mutant enzyme C404A, with D-glyceraldehyde as cosubstrate,at pH 7.5 and 37°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glyceraldehyde | Homo sapiens | - |
ADP + D-glyceraldehyde 3-phosphate | - |
? | |
ATP + dihydroxyacetone | Homo sapiens | - |
ADP + dihydroxyacetone phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q3LXA3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glyceraldehyde | - |
Homo sapiens | ADP + D-glyceraldehyde 3-phosphate | - |
? | |
ATP + dihydroxyacetone | - |
Homo sapiens | ADP + dihydroxyacetone phosphate | - |
? | |
additional information | no activity with glycolaldehyde and glycerol | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
dAK | - |
Homo sapiens |
dAK | the gene encodes triokinase and FMN cyclase | Homo sapiens |
TKFC | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
dihydroxyacetone | mutant enzyme S446A, at pH 7.5 and 37°C | Homo sapiens | |
0.15 | - |
ATP | mutant enzyme S446A, with dihydroxyacetone as cosubstrate, at pH 7.5 and 37°C | Homo sapiens | |
0.16 | - |
D-glyceraldehyde | mutant enzyme T112A, at pH 7.5 and 37°C | Homo sapiens | |
0.16 | - |
ATP | mutant enzyme T112A, with D-glyceraldehyde as cosubstrate, at pH 7.5 and 37°C | Homo sapiens | |
0.5 | - |
ATP | mutant enzyme T112A, with dihydroxyacetone as cosubstrate, at pH 7.5 and 37°C | Homo sapiens | |
0.51 | - |
dihydroxyacetone | mutant enzyme T112A, at pH 7.5 and 37°C | Homo sapiens | |
0.66 | - |
ATP | mutant enzyme S446A, with D-glyceraldehyde as cosubstrate, at pH 7.5 and 37°C | Homo sapiens | |
0.74 | - |
D-glyceraldehyde | mutant enzyme S446A, at pH 7.5 and 37°C | Homo sapiens | |
1.7 | - |
ATP | mutant enzyme K204A, with D-glyceraldehyde as cosubstrate, at pH 7.5 and 37°C | Homo sapiens | |
2.09 | - |
D-glyceraldehyde | mutant enzyme K204A, at pH 7.5 and 37°C | Homo sapiens | |
2.1 | - |
ATP | mutant enzyme C404A, with D-glyceraldehyde as cosubstrate,at pH 7.5 and 37°C | Homo sapiens | |
2.17 | - |
ATP | mutant enzyme C404A, with dihydroxyacetone as cosubstrate, at pH 7.5 and 37°C | Homo sapiens | |
2.26 | - |
dihydroxyacetone | mutant enzyme C404A, at pH 7.5 and 37°C | Homo sapiens | |
2.27 | - |
D-glyceraldehyde | mutant enzyme C404A, at pH 7.5 and 37°C | Homo sapiens | |
2.34 | - |
ATP | mutant enzyme K204A, with dihydroxyacetone as cosubstrate, at pH 7.5 and 37°C | Homo sapiens | |
2.79 | - |
dihydroxyacetone | mutant enzyme K204A, at pH 7.5 and 37°C | Homo sapiens | |
3.94 | - |
ATP | wild type enzyme, with D-glyceraldehyde as cosubstrate, at pH 7.5 and 37°C | Homo sapiens | |
4.59 | - |
ATP | wild type enzyme, with dihydroxyacetone as cosubstrate, at pH 7.5 and 37°C | Homo sapiens | |
4.83 | - |
D-glyceraldehyde | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens | |
5 | - |
dihydroxyacetone | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
ATP | mutant enzyme T112A, with D-glyceraldehyde as cosubstrate, at pH 7.5 and 37°C | Homo sapiens | |
0.64 | - |
ATP | mutant enzyme T112A, with dihydroxyacetone as cosubstrate, at pH 7.5 and 37°C | Homo sapiens | |
1.15 | - |
ATP | mutant enzyme C404A, with D-glyceraldehyde as cosubstrate,at pH 7.5 and 37°C | Homo sapiens | |
1.2 | - |
D-glyceraldehyde | mutant enzyme T112A, at pH 7.5 and 37°C | Homo sapiens | |
1.9 | - |
ATP | mutant enzyme S446A, with D-glyceraldehyde as cosubstrate, at pH 7.5 and 37°C | Homo sapiens | |
2.18 | - |
ATP | mutant enzyme C404A, with dihydroxyacetone as cosubstrate, at pH 7.5 and 37°C | Homo sapiens | |
2.4 | - |
ATP | mutant enzyme K204A, with D-glyceraldehyde as cosubstrate, at pH 7.5 and 37°C | Homo sapiens | |
2.6 | - |
ATP | mutant enzyme S446A, with dihydroxyacetone as cosubstrate, at pH 7.5 and 37°C | Homo sapiens | |
4.2 | - |
ATP | mutant enzyme K204A, with dihydroxyacetone as cosubstrate, at pH 7.5 and 37°C | Homo sapiens | |
6.3 | - |
ATP | wild type enzyme, with D-glyceraldehyde as cosubstrate, at pH 7.5 and 37°C | Homo sapiens | |
7.6 | - |
dihydroxyacetone | mutant enzyme T112A, at pH 7.5 and 37°C | Homo sapiens | |
10.4 | - |
D-glyceraldehyde | mutant enzyme S446A, at pH 7.5 and 37°C | Homo sapiens | |
10.5 | - |
ATP | wild type enzyme, with dihydroxyacetone as cosubstrate, at pH 7.5 and 37°C | Homo sapiens | |
13.1 | - |
dihydroxyacetone | mutant enzyme S446A, at pH 7.5 and 37°C | Homo sapiens | |
14 | - |
D-glyceraldehyde | mutant enzyme C404A, at pH 7.5 and 37°C | Homo sapiens | |
14.2 | - |
D-glyceraldehyde | mutant enzyme K204A, at pH 7.5 and 37°C | Homo sapiens | |
27.5 | - |
D-glyceraldehyde | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens | |
115 | - |
dihydroxyacetone | mutant enzyme K204A, at pH 7.5 and 37°C | Homo sapiens | |
118 | - |
dihydroxyacetone | mutant enzyme C404A, at pH 7.5 and 37°C | Homo sapiens | |
321 | - |
dihydroxyacetone | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens |