Literature summary for 2.7.1.26 extracted from

Karthikeyan, S.; Zhou, Q.; Osterman, A.L.; Zhang, H.
Ligand binding-induced conformational changes in riboflavin kinase: structural basis for the ordered mechanism (2003), Biochemistry, 42, 12532-12538.

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme with bound products FMN and MgADP, hanging drop vapour diffusion method, 34 mg/ml protein in 50 mM Tris, pH 7.4, 0.3 M NaCl, 1 mM DTT, 20°C, mixing with equal volume of reservoir solution containing 0.1 M sodium acetate, pH 4.7, 30% PEG monomethyl ether 5000, and 0.2 M ammonium sulfate, followed by microseeding in reservoir solution containing 0.1 M sodium acetate, pH 4.4, 22.5% PEG monomethyl ether 5000, and 0.2 M ammonium sulfate, cryoprotection in 30% glycerol in reservoir solution, storage in liquid propane, X-ray diffraction structure determination and analysis at 2.4 A resolution	Homo sapiens

Crystallization (Comment)

Organism

purified recombinant enzyme with bound products FMN and MgADP, hanging drop vapour diffusion method, 34 mg/ml protein in 50 mM Tris, pH 7.4, 0.3 M NaCl, 1 mM DTT, 20°C, mixing with equal volume of reservoir solution containing 0.1 M sodium acetate, pH 4.7, 30% PEG monomethyl ether 5000, and 0.2 M ammonium sulfate, followed by microseeding in reservoir solution containing 0.1 M sodium acetate, pH 4.4, 22.5% PEG monomethyl ether 5000, and 0.2 M ammonium sulfate, cryoprotection in 30% glycerol in reservoir solution, storage in liquid propane, X-ray diffraction structure determination and analysis at 2.4 A resolution

Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + riboflavin	Homo sapiens	-	ADP + FMN	-	ir

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q969G6	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant, His-tagged enzyme by nickel affinity chromatography, removal of His-tag by TEV-protease treatment	Homo sapiens

Reaction

Reaction	Comment	Organism	Reaction ID
ATP + riboflavin = ADP + FMN	ordered reaction mechanism, FMN binding o the binary enzyme-MgADP complex induces important conformational changes	Homo sapiens	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + riboflavin	-	Homo sapiens	ADP + FMN	-	ir
ATP + riboflavin	substrate and product binding structure, overview	Homo sapiens	ADP + FMN	-	ir

Synonyms

Synonyms	Comment	Organism
RFK	-	Homo sapiens