Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | the enzyme activity is about 3fold stimulated in the presence of 2 mM dithiothreitol | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
in complex with ligands, hanging drop vapor diffusion method, using 0.2 M NH4NO3, 20% (w/v) PEG3350 | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
C541A | the mutant shows reduced activity compared to the wild type enzyme | Mycobacterium tuberculosis |
C549A | the mutant shows increased activity compared to the wild type enzyme | Mycobacterium tuberculosis |
C556S | inactive | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
- |
Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + adenosine-5'-phosphosulfate | Mycobacterium tuberculosis | - |
ADP + 3'-phosphoadenosine 5'-phosphosulfate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WNM5 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography and Superdex 200 gel filtration | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + adenosine-5'-phosphosulfate | - |
Mycobacterium tuberculosis | ADP + 3'-phosphoadenosine 5'-phosphosulfate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 21260, electrospray ionization mass spectrometry | Mycobacterium tuberculosis |
homodimer | 2 * 21261, calculated from amino acid sequence | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
CysC | - |
Mycobacterium tuberculosis |