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Literature summary for 2.7.1.25 extracted from

  • Poyraz, O.e.; Brunner, K.; Lohkamp, B.; Axelsson, H.; Hammarstroem, L.G.; Schnell, R.; Schneider, G.
    Crystal structures of the kinase domain of the sulfate-activating complex in Mycobacterium tuberculosis (2015), PLoS ONE, 10, e0121494.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.25

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol the enzyme activity is about 3fold stimulated in the presence of 2 mM dithiothreitol Mycobacterium tuberculosis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with ligands, hanging drop vapor diffusion method, using 0.2 M NH4NO3, 20% (w/v) PEG3350 Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
C541A the mutant shows reduced activity compared to the wild type enzyme Mycobacterium tuberculosis
C549A the mutant shows increased activity compared to the wild type enzyme Mycobacterium tuberculosis
C556S inactive Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
-
 Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + adenosine-5'-phosphosulfate Mycobacterium tuberculosis
-
 ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WNM5
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography and Superdex 200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + adenosine-5'-phosphosulfate
-
 Mycobacterium tuberculosis ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 21260, electrospray ionization mass spectrometry Mycobacterium tuberculosis
homodimer 2 * 21261, calculated from amino acid sequence Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
CysC
-
 Mycobacterium tuberculosis