Literature summary for 2.7.1.25 extracted from

Sekulic, N.; Dietrich, K.; Paarmann, I.; Ort, S.; Konrad, M.; Lavie, A.
Elucidation of the active conformation of the APS-kinase domain of human PAPS synthetase 1 (2007), J. Mol. Biol., 367, 488-500.

Search for more literature for 2.7.1.25

Cloned(Commentary)

Cloned (Comment)	Organism
the APS-kinase domain (25-227 amino acid resiudes) of the PAPSS1 coding region are expressed as a GST-fusion protein in Escherichia coli BL-21	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structures of the PAPSS1 APS-kinase domain in complex with APS and in complex with the products PAPS and ADP are solved, both structures of isolated domain, obtained in different crystal forms, reveal symmetrical dimers	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
EDTA	assay with 0.25 mM EDTA	Homo sapiens
K+	assay with 100 mM KCl	Homo sapiens
Mg2+	assay with 2.5 mM MgCl2	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
protein solution is loaded on a Glutathione-Sepharose Fast Flow column and further purified by gel-filtration chromatography using Superdex 200	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
MgATP2- + adenylyl sulfate	-	Homo sapiens	MgADP- + 3'-phosphoadenylyl sulfate	-	r

Synonyms

Synonyms	Comment	Organism
3'-phosphoadenosine 5'-phosphosulfate synthetase1	-	Homo sapiens
PAPSS1	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Homo sapiens

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Release 2023.1 (January 2023)