Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | - |
Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed as His-tag fusion protein in Escherichia coli BL21(DE3) | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine 5'-phosphosulfate | substrate inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0042 | - |
adenosine 5'-phosphosulfate | pH 8.0, 30°C | Homo sapiens | |
0.14 | - |
ATP | pH 8.0, 30°C | Homo sapiens | |
0.14 | - |
ATP | ATP in form of MgATP2- | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | in complex with ATP | Homo sapiens | |
NaCl | increased activity at high salt conditions, 100 mM | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
71000 | - |
2 * 71000 | Homo sapiens |
150000 | - |
gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + adenosine 5'-phosphosulfate | Homo sapiens | synthesis of the important sulfate donor 3'-phosphoadenosine 5'-phosphosulfate from ATP and sulfate | ADP + 3'-phosphoadenosine 5'-phosphosulfate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O43252 | isoform 1 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme using His-tag | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.12 | - |
pH 8.0. 30°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + adenosine 5'-phosphosulfate | - |
Homo sapiens | ADP + 3'-phosphoadenosine 5'-phosphosulfate | - |
? | |
ATP + adenosine 5'-phosphosulfate | synthesis of the important sulfate donor 3'-phosphoadenosine 5'-phosphosulfate from ATP and sulfate | Homo sapiens | ADP + 3'-phosphoadenosine 5'-phosphosulfate | - |
? | |
ATP + adenylyl sulfate | - |
Homo sapiens | ADP + 3'-phosphoadenylyl sulfate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 71000 | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
More | bifunctional enzyme with ATP sulfurylase activity and adenosine phosphosulfate kinase activity, catalyzes the formation of 3'-phosphoadenosine 5'-phosphosulfate from ATP and sulfate | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.048 | - |
adenosine 5'-phosphosulfate | pH 8.0, 30°C | Homo sapiens |