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Literature summary for 2.7.1.25 extracted from

  • Schriek, U.; Schwenn, J.D.
    Properties of the purified APS-kinase from Escherichia coli and Saccharomyces cerevisiae (1986), Arch. Microbiol., 145, 32-38.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ascorbate activation Escherichia coli
dithiothreitol activation Escherichia coli
dithiothreitol activation Saccharomyces cerevisiae
glutathione reduced glutathione increases the activity significantly Escherichia coli
glutathione reduced glutathione increases the activity significantly Saccharomyces cerevisiae
L-cysteine activation Escherichia coli
additional information not activated by 2-mercaptoethanol Escherichia coli
additional information not activated by 2-mercaptoethanol Saccharomyces cerevisiae
additional information not activated by L-cysteine and ascorbate Saccharomyces cerevisiae
thioredoxin activation in the presence of dithiothreitol Escherichia coli
thioredoxin activation in the presence of dithiothreitol Saccharomyces cerevisiae

General Stability

General Stability Organism
rapid loss of activity in dilute solutions Escherichia coli
rapid loss of activity in dilute solutions Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
2,4,6-Trinitrobenzene sulfonate 0.05 mM, complete inhibition Escherichia coli
2,4,6-Trinitrobenzene sulfonate 0.05 mM, 28% inhibition Saccharomyces cerevisiae
2,6-dichlorophenol indophenol 0.05 mM, complete inhibition Escherichia coli
2,6-dichlorophenol indophenol 0.05 mM, complete inhibition Saccharomyces cerevisiae
Bromosuccinimide 0.05 mM, complete inhibition Escherichia coli
Bromosuccinimide 0.05 mM, complete inhibition Saccharomyces cerevisiae
dehydroascorbate
-
Escherichia coli
dehydroascorbate
-
Saccharomyces cerevisiae
FAD
-
Escherichia coli
FAD
-
Saccharomyces cerevisiae
ferricyanide 0.05 mM, complete inhibition Escherichia coli
GSSG
-
Escherichia coli
GSSG
-
Saccharomyces cerevisiae
Mercuriphenylacetate 0.05 mM, complete inhibition Escherichia coli
Mercuriphenylacetate 0.05 mM, complete inhibition Saccharomyces cerevisiae
additional information
-
Escherichia coli
additional information not inhibited by ferricyanide Saccharomyces cerevisiae
oxidized glutathione thioredoxin reverses inactivation Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Escherichia coli 5829
-
cytosol
-
Saccharomyces cerevisiae 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29500
-
in the presence of dithiothreitol, gel filtration Saccharomyces cerevisiae
40000 45000 gel filtration Saccharomyces cerevisiae
49500 52000 gel filtration Saccharomyces cerevisiae
85000 90000 gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli AN1460
-
-
-
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, Blue Sepharose, Sephacryl S-200, PBE 94, Red Sepharose, phenyl-sepharose Saccharomyces cerevisiae
ammonium sulfate, Giogel P2, Blue Sepharose, Sephacryl S-300, PBE 94 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.614
-
-
Escherichia coli
0.614
-
-
Saccharomyces cerevisiae
0.843
-
-
Escherichia coli
0.843
-
-
Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS Escherichia coli ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate ?
ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS Saccharomyces cerevisiae ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate ?
ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS Escherichia coli AN1460 ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate ?

Subunits

Subunits Comment Organism
dimer 2 * 28000-29500, dithiothreitol-treated enzyme, SDS-PAGE Saccharomyces cerevisiae
dimer 2 * 40000-45000, dithiothreitol-treated enzyme, SDS-PAGE Escherichia coli
tetramer monomers and dimers are catalytically active Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
pI: 5.5 Escherichia coli
additional information
-
pI: 5.5 Saccharomyces cerevisiae
additional information
-
pI: 7.4 Escherichia coli
additional information
-
pI: 7.4 Saccharomyces cerevisiae
6.8 7
-
Escherichia coli
6.8 7
-
Saccharomyces cerevisiae
8
-
-
Escherichia coli
8
-
-
Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
6 8 continuous increase of activity from pH 6.0 to 8.0 Escherichia coli
6 8 continuous increase of activity from pH 6.0 to 8.0 Saccharomyces cerevisiae
6.8 8 maximal activity at pH 6.8, approx. half-maximal activity at pH 8.0 Escherichia coli
6.8 8 maximal activity at pH 6.8, approx. half-maximal activity at pH 8.0 Saccharomyces cerevisiae