Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbate | activation | Escherichia coli | |
dithiothreitol | activation | Escherichia coli | |
dithiothreitol | activation | Saccharomyces cerevisiae | |
glutathione | reduced glutathione increases the activity significantly | Escherichia coli | |
glutathione | reduced glutathione increases the activity significantly | Saccharomyces cerevisiae | |
L-cysteine | activation | Escherichia coli | |
additional information | not activated by 2-mercaptoethanol | Escherichia coli | |
additional information | not activated by 2-mercaptoethanol | Saccharomyces cerevisiae | |
additional information | not activated by L-cysteine and ascorbate | Saccharomyces cerevisiae | |
thioredoxin | activation in the presence of dithiothreitol | Escherichia coli | |
thioredoxin | activation in the presence of dithiothreitol | Saccharomyces cerevisiae |
General Stability | Organism |
---|---|
rapid loss of activity in dilute solutions | Escherichia coli |
rapid loss of activity in dilute solutions | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4,6-Trinitrobenzene sulfonate | 0.05 mM, complete inhibition | Escherichia coli | |
2,4,6-Trinitrobenzene sulfonate | 0.05 mM, 28% inhibition | Saccharomyces cerevisiae | |
2,6-dichlorophenol indophenol | 0.05 mM, complete inhibition | Escherichia coli | |
2,6-dichlorophenol indophenol | 0.05 mM, complete inhibition | Saccharomyces cerevisiae | |
Bromosuccinimide | 0.05 mM, complete inhibition | Escherichia coli | |
Bromosuccinimide | 0.05 mM, complete inhibition | Saccharomyces cerevisiae | |
dehydroascorbate | - |
Escherichia coli | |
dehydroascorbate | - |
Saccharomyces cerevisiae | |
FAD | - |
Escherichia coli | |
FAD | - |
Saccharomyces cerevisiae | |
ferricyanide | 0.05 mM, complete inhibition | Escherichia coli | |
GSSG | - |
Escherichia coli | |
GSSG | - |
Saccharomyces cerevisiae | |
Mercuriphenylacetate | 0.05 mM, complete inhibition | Escherichia coli | |
Mercuriphenylacetate | 0.05 mM, complete inhibition | Saccharomyces cerevisiae | |
additional information | - |
Escherichia coli | |
additional information | not inhibited by ferricyanide | Saccharomyces cerevisiae | |
oxidized glutathione | thioredoxin reverses inactivation | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Escherichia coli | 5829 | - |
cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29500 | - |
in the presence of dithiothreitol, gel filtration | Saccharomyces cerevisiae |
40000 | 45000 | gel filtration | Saccharomyces cerevisiae |
49500 | 52000 | gel filtration | Saccharomyces cerevisiae |
85000 | 90000 | gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli AN1460 | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, Blue Sepharose, Sephacryl S-200, PBE 94, Red Sepharose, phenyl-sepharose | Saccharomyces cerevisiae |
ammonium sulfate, Giogel P2, Blue Sepharose, Sephacryl S-300, PBE 94 | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.614 | - |
- |
Escherichia coli |
0.614 | - |
- |
Saccharomyces cerevisiae |
0.843 | - |
- |
Escherichia coli |
0.843 | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + adenosine 5-phosphosulfate | i.e. adenylylsulfate or APS | Escherichia coli | ADP + 3'-phosphoadenosine 5'-phosphosulfate | i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate | ? | |
ATP + adenosine 5-phosphosulfate | i.e. adenylylsulfate or APS | Saccharomyces cerevisiae | ADP + 3'-phosphoadenosine 5'-phosphosulfate | i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate | ? | |
ATP + adenosine 5-phosphosulfate | i.e. adenylylsulfate or APS | Escherichia coli AN1460 | ADP + 3'-phosphoadenosine 5'-phosphosulfate | i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate | ? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 28000-29500, dithiothreitol-treated enzyme, SDS-PAGE | Saccharomyces cerevisiae |
dimer | 2 * 40000-45000, dithiothreitol-treated enzyme, SDS-PAGE | Escherichia coli |
tetramer | monomers and dimers are catalytically active | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 5.5 | Escherichia coli |
additional information | - |
pI: 5.5 | Saccharomyces cerevisiae |
additional information | - |
pI: 7.4 | Escherichia coli |
additional information | - |
pI: 7.4 | Saccharomyces cerevisiae |
6.8 | 7 | - |
Escherichia coli |
6.8 | 7 | - |
Saccharomyces cerevisiae |
8 | - |
- |
Escherichia coli |
8 | - |
- |
Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | continuous increase of activity from pH 6.0 to 8.0 | Escherichia coli |
6 | 8 | continuous increase of activity from pH 6.0 to 8.0 | Saccharomyces cerevisiae |
6.8 | 8 | maximal activity at pH 6.8, approx. half-maximal activity at pH 8.0 | Escherichia coli |
6.8 | 8 | maximal activity at pH 6.8, approx. half-maximal activity at pH 8.0 | Saccharomyces cerevisiae |