Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithioerythritol | 2 mM, 200% activation | Chlamydomonas reinhardtii | |
dithiothreitol | activation | Chlamydomonas reinhardtii | |
dithiothreitol | 2 mM, 200% activation | Chlamydomonas reinhardtii | |
additional information | not activated by 2-mercaptoethanol | Chlamydomonas reinhardtii | |
Na2SO3 | 5 mM, 200% activation | Chlamydomonas reinhardtii |
General Stability | Organism |
---|---|
more stable in ampholyte polybuffer 74 than in Tris or imidazole buffers | Chlamydomonas reinhardtii |
slow freezing can cause loss of activity | Chlamydomonas reinhardtii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-Aminophenylacetate | 0.008 mM, 50% inhibition | Chlamydomonas reinhardtii | |
AMP | - |
Chlamydomonas reinhardtii | |
Bromosuccinimide | strong inhibition | Chlamydomonas reinhardtii | |
CMP | - |
Chlamydomonas reinhardtii | |
Co2+ | 2 mM, 63% inhibition | Chlamydomonas reinhardtii | |
Cu2+ | 2 mM, 90% inhibition | Chlamydomonas reinhardtii | |
GMP | - |
Chlamydomonas reinhardtii | |
N-ethylmaleimide | 0.02 mM, 50% inhibition | Chlamydomonas reinhardtii | |
p-chloromercuribenzoate | 0.005 mM, 50% inhibition | Chlamydomonas reinhardtii | |
UMP | - |
Chlamydomonas reinhardtii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
adenosine 5'-phosphosulfate | pH 6.8, 30°C | Chlamydomonas reinhardtii | |
0.007 | - |
ATP | pH 6.8, 30°C | Chlamydomonas reinhardtii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | 10 mM, 60% stimulation compared to Mg2+ | Chlamydomonas reinhardtii | |
Mg2+ | activation | Chlamydomonas reinhardtii | |
Mn2+ | - |
Chlamydomonas reinhardtii | |
additional information | not activated by Cu2+ or Na+ | Chlamydomonas reinhardtii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
x * 44000, SDS-PAGE | Chlamydomonas reinhardtii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | - |
CW15, cell wall mutant | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, Sephacryl S-300, chromatofocusing, 2'5'-ADP-agarose | Chlamydomonas reinhardtii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.136 | - |
- |
Chlamydomonas reinhardtii |
Storage Stability | Organism |
---|---|
-20°C, stable to prolonged storage | Chlamydomonas reinhardtii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + adenosine 5-phosphosulfate | ATP can be replaced by GTP, ITP or UTP | Chlamydomonas reinhardtii | ADP + 3'-phosphoadenosine 5'-phosphosulfate | i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate | r | |
ATP + adenosine 5-phosphosulfate | GTP, ITP or UTP are poor substrates | Chlamydomonas reinhardtii | ADP + 3'-phosphoadenosine 5'-phosphosulfate | i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate | r | |
ATP + adenosine 5-phosphosulfate | i.e. adenylylsulfate or APS | Chlamydomonas reinhardtii | ADP + 3'-phosphoadenosine 5'-phosphosulfate | i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate | r | |
CTP + adenosine 5-phosphosulfate | - |
Chlamydomonas reinhardtii | CDP + 3'-phosphoadenosine 5'-phosphosulfate | - |
? | |
dATP + adenosine 5-phosphosulfate | - |
Chlamydomonas reinhardtii | dADP + 3'-phosphoadenosine 5'-phosphosulfate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 44000, SDS-PAGE | Chlamydomonas reinhardtii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Chlamydomonas reinhardtii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 6.2 | Chlamydomonas reinhardtii |
6 | - |
- |
Chlamydomonas reinhardtii |
6.5 | 7.5 | in MES-Tricine-HEPES buffer | Chlamydomonas reinhardtii |
6.8 | - |
in imidazole buffer | Chlamydomonas reinhardtii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | approx. 80% of maximal activity at pH 5.5, approx. half-maximal activity at pH 8.5 | Chlamydomonas reinhardtii |
6 | 8 | approx. 80% of maximal activity at pH 6.0, approx. 65% of maximal activity at pH 8.0, imidazole buffer | Chlamydomonas reinhardtii |
6 | 8.5 | approx. 70% of maximal activity at pH 6.0, approx. 75% of maximal activity at pH 8.5, MES-Tricine-HEPES buffer | Chlamydomonas reinhardtii |