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Literature summary for 2.7.1.25 extracted from

  • Schwenn, J.D.; Jender, H.G.
    Purification and properties of the ATP:adenylylsulfate 3'-phosphotransferase from chlamydomonas reinhardii (1984), Arch. Microbiol., 138, 9-14.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
dithioerythritol 2 mM, 200% activation Chlamydomonas reinhardtii
dithiothreitol activation Chlamydomonas reinhardtii
dithiothreitol 2 mM, 200% activation Chlamydomonas reinhardtii
additional information not activated by 2-mercaptoethanol Chlamydomonas reinhardtii
Na2SO3 5 mM, 200% activation Chlamydomonas reinhardtii

General Stability

General Stability Organism
more stable in ampholyte polybuffer 74 than in Tris or imidazole buffers Chlamydomonas reinhardtii
slow freezing can cause loss of activity Chlamydomonas reinhardtii

Inhibitors

Inhibitors Comment Organism Structure
4-Aminophenylacetate 0.008 mM, 50% inhibition Chlamydomonas reinhardtii
AMP
-
Chlamydomonas reinhardtii
Bromosuccinimide strong inhibition Chlamydomonas reinhardtii
CMP
-
Chlamydomonas reinhardtii
Co2+ 2 mM, 63% inhibition Chlamydomonas reinhardtii
Cu2+ 2 mM, 90% inhibition Chlamydomonas reinhardtii
GMP
-
Chlamydomonas reinhardtii
N-ethylmaleimide 0.02 mM, 50% inhibition Chlamydomonas reinhardtii
p-chloromercuribenzoate 0.005 mM, 50% inhibition Chlamydomonas reinhardtii
UMP
-
Chlamydomonas reinhardtii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
adenosine 5'-phosphosulfate pH 6.8, 30°C Chlamydomonas reinhardtii
0.007
-
ATP pH 6.8, 30°C Chlamydomonas reinhardtii

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 10 mM, 60% stimulation compared to Mg2+ Chlamydomonas reinhardtii
Mg2+ activation Chlamydomonas reinhardtii
Mn2+
-
Chlamydomonas reinhardtii
additional information not activated by Cu2+ or Na+ Chlamydomonas reinhardtii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
x * 44000, SDS-PAGE Chlamydomonas reinhardtii

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
-
CW15, cell wall mutant
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, Sephacryl S-300, chromatofocusing, 2'5'-ADP-agarose Chlamydomonas reinhardtii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.136
-
-
Chlamydomonas reinhardtii

Storage Stability

Storage Stability Organism
-20°C, stable to prolonged storage Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + adenosine 5-phosphosulfate ATP can be replaced by GTP, ITP or UTP Chlamydomonas reinhardtii ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate r
ATP + adenosine 5-phosphosulfate GTP, ITP or UTP are poor substrates Chlamydomonas reinhardtii ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate r
ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS Chlamydomonas reinhardtii ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate r
CTP + adenosine 5-phosphosulfate
-
Chlamydomonas reinhardtii CDP + 3'-phosphoadenosine 5'-phosphosulfate
-
?
dATP + adenosine 5-phosphosulfate
-
Chlamydomonas reinhardtii dADP + 3'-phosphoadenosine 5'-phosphosulfate
-
?

Subunits

Subunits Comment Organism
? x * 44000, SDS-PAGE Chlamydomonas reinhardtii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Chlamydomonas reinhardtii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
pI: 6.2 Chlamydomonas reinhardtii
6
-
-
Chlamydomonas reinhardtii
6.5 7.5 in MES-Tricine-HEPES buffer Chlamydomonas reinhardtii
6.8
-
in imidazole buffer Chlamydomonas reinhardtii

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8.5 approx. 80% of maximal activity at pH 5.5, approx. half-maximal activity at pH 8.5 Chlamydomonas reinhardtii
6 8 approx. 80% of maximal activity at pH 6.0, approx. 65% of maximal activity at pH 8.0, imidazole buffer Chlamydomonas reinhardtii
6 8.5 approx. 70% of maximal activity at pH 6.0, approx. 75% of maximal activity at pH 8.5, MES-Tricine-HEPES buffer Chlamydomonas reinhardtii