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Literature summary for 2.7.1.25 extracted from
- APS kinase from Penicillium chrysogenum. Dissociation and reassociation of subunits as the basis of the reversible heat inactivation (1985), J. Biol. Chem., 260, 1535-1544.
General Stability
| General Stability | Organism |
|---|---|
| slow inactivation in dilute solutions, even at low temperatures | Penicillium chrysogenum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,4,6-Trinitrobenzene sulfonate | in the presence or absence of ATP-sulfurylase | Penicillium chrysogenum |  |
| diethyldicarbonate | in the presence or absence of ATP-sulfurylase | Penicillium chrysogenum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activation | Penicillium chrysogenum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
2 * 33000, gel filtration at 46°C, the enzyme dissociates into two inactive monomers by heating above 42°C | Penicillium chrysogenum |
| 57000 | - |
gel filtration at 22°C | Penicillium chrysogenum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium chrysogenum | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Penicillium chrysogenum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 24.4 | - |
- |
Penicillium chrysogenum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + adenosine 5-phosphosulfate | i.e. adenylylsulfate or APS | Penicillium chrysogenum | ADP + 3'-phosphoadenosine 5'-phosphosulfate | i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate | r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 33000, gel filtration at 46°C, the enzyme dissociates into two inactive monomers by heating above 42°C | Penicillium chrysogenum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Penicillium chrysogenum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 36 | - |
equilibrium between active and inactive enzyme form | Penicillium chrysogenum |
| 40 | - |
reversible inactivation above by subunit dissociation, kinetics, MgATP2- or MgADP- stimulate reactivation | Penicillium chrysogenum |
| 43 | - |
reversible inactivation, t1/2: 1 min | Penicillium chrysogenum |
| 50 | - |
1 min, 80% reversible inactivation | Penicillium chrysogenum |
| 60 | 100 | 1 min, 85% reversible inactivation | Penicillium chrysogenum |
| 80 | - |
irreversible inactivation, t1/2: 47 min, pH 8, 0.023 mg protein/ml | Penicillium chrysogenum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
below, reversible inactivation at 30°C, reactivation rate increases with increasing pH | Penicillium chrysogenum |
| 7 | - |
reversible inactivation at 42°C | Penicillium chrysogenum |
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