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Literature summary for 2.7.1.235 extracted from
- Purification and characterization of WaaP from Escherichia coli, a lipopolysaccharide kinase essential for outer membrane stability (2001), J. Biol. Chem., 276, 5498-5504 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D162A | mutant shows negligible kinase activity | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.076 | - |
an alpha-Hep-(1->3)-alpha-Hep-(1->5)-[alpha-Kdo-(2->4)]-alpha-Kdo-(2->6)-[lipid A] | pH 7.5, 35°C | Escherichia coli |  |
| 0.13 | - |
ATP | pH 7.5, 35°C | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activity is absolutely dependent upon the presence of Mg2+, maximum activity at 17.5 mM | Escherichia coli | |
| additional information | Ca2+ cannot substitute for Mg2+ | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + an alpha-Hep-(1->3)-alpha-Hep-(1->5)-[alpha-Kdo-(2->4)]-alpha-Kdo-(2->6)-[lipid A] | Escherichia coli | - |
ADP + an alpha-Hep-(1->3)-4-O-phospho-alpha-Hep-(1->5)-[alpha-Kdo-(2->4)]-alpha-Kdo-(2->6)-[lipid A] | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | Q9R9D6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein, purified by immobilized metal affinity chromatography and subtractive anion exchange chromatography | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + an alpha-Hep-(1->3)-alpha-Hep-(1->5)-[alpha-Kdo-(2->4)]-alpha-Kdo-(2->6)-[lipid A] | - |
Escherichia coli | ADP + an alpha-Hep-(1->3)-4-O-phospho-alpha-Hep-(1->5)-[alpha-Kdo-(2->4)]-alpha-Kdo-(2->6)-[lipid A] | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 31049, calculated from sequence, x * 33000, SDS-PAGE of recombinant His-tagged protein | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RfaP | - |
Escherichia coli |
| WaaP | - |
Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | - |
Escherichia coli |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Escherichia coli | calculated from sequence | - |
9.8 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | WaaP is required for the addition of phosphate to O-4 of the first heptose residue of the lipopolysaccharide inner core region. WaaP is necessary for resistance to hydrophobic and polycationic antimicrobials in Escherichia coli | Escherichia coli |
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