Literature summary for 2.7.1.232 extracted from

Rother, C.; Gutmann, A.; Gudiminchi, R.; Weber, H.; Lepak, A.; Nidetzky, B.
Biochemical characterization and mechanistic analysis of the levoglucosan kinase from Lipomyces starkeyi (2018), ChemBioChem, 19, 596-603 .

Search for more literature for 2.7.1.232

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Lipomyces starkeyi

Inhibitors

Inhibitors	Comment	Organism	Structure
ADP	strong competitive inhibitor against ATP	Lipomyces starkeyi
D-Glucose-6-phosphate	-	Lipomyces starkeyi

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1	-	ATP	at pH 7.8 and 30Â°C	Lipomyces starkeyi
177	-	1,6-anhydro-beta-D-glucopyranose	at pH 7.8 and 30Â°C	Lipomyces starkeyi

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	the enzyme contains two Mg2+ ions. Using 4 and 8 mM Mg2+, the activity is highest at a Mg2+/ATP ratio of 2 and drastically drops if the ratio is less than 1. Employing a constant ATP concentration (2.5 mM), the enzyme activity increases with the Mg2+ concentration until a Mg2+/ATP ratio of 8 is reached at 20 mM MgCl2	Lipomyces starkeyi

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + 1,6-anhydro-beta-D-glucopyranose + H2O	Lipomyces starkeyi	-	ADP + alpha-D-glucose-6-phosphate	-	ir

Organism

Organism	UniProt	Comment	Textmining
Lipomyces starkeyi	B3VI55	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 1,6-anhydro-beta-D-glucopyranose + H2O	-	Lipomyces starkeyi	ADP + alpha-D-glucose-6-phosphate	-	ir
ATP + 1,6-anhydro-beta-D-glucopyranose + H2O	1,6-anhydro-beta-D-glucopyranose is levoglucosan. The enzyme is highly specific for levoglucosan	Lipomyces starkeyi	ADP + alpha-D-glucose-6-phosphate	-	ir
additional information	no enzymatic back reaction from alpha-D-glucose-6-phosphate and ADP is observed. The enzyme also shows no activity with ADP plus 6-phospho-alpha-D-glucopyranosyl fluoride or 6-phospho-D-glucal	Lipomyces starkeyi	?	-	-

Synonyms

Synonyms	Comment	Organism
LGK	-	Lipomyces starkeyi

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
25	37	at 30Â°C and pH 7.5, all enzyme activity is lost within 2 h. A temperature decrease to 25Â°C and the addition of 1 mg/ml bovine serum albumin enhance the enzyme's half-life to approximately 4 h. The enzyme's melting temperature is 37Â°C	Lipomyces starkeyi

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Lipomyces starkeyi

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.45	-	ADP	at pH 7.8 and 30Â°C	Lipomyces starkeyi
56	-	D-Glucose-6-phosphate	at pH 7.8 and 30Â°C	Lipomyces starkeyi

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Release 2023.1 (January 2023)