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Literature summary for 2.7.1.23 extracted from

  • Ohashi, K.; Kawai, S.; Murata, K.
    Identification and characterization of a human mitochondrial NAD kinase (2012), Nat. Commun., 3, 1248.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.23

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta-gami(DE3)pLysS cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
NADH 34% residual activity at 0.5 mM NADH Homo sapiens
NADP+ 58% residual activity at 0.5 mM NADP+ Homo sapiens
NADPH 17% residual activity at 0.5 mM NADPH Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
 NAD+ in 100 mM Tris-HCl (pH 8.0), at 37°C Homo sapiens
1.7
-
 ATP in 100 mM Tris-HCl (pH 8.0), at 37°C Homo sapiens
12
-
 Tetrapolyphosphate in 100 mM Tris-HCl (pH 8.0), at 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Homo sapiens 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 2 * 43000, SDS-PAGE Homo sapiens
43300
-
 2 * 43300, calculated from amino acid sequence Homo sapiens
75000
-
 gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Talon column chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Homo sapiens
-
colon
-
 Homo sapiens
-
heart highest expression Homo sapiens
-
HEK-293A cell
-
 Homo sapiens
-
kidney
-
 Homo sapiens
-
liver
-
 Homo sapiens
-
lung
-
 Homo sapiens
-
placenta
-
 Homo sapiens
-
skeletal muscle lowest expression Homo sapiens
-
small intestine
-
 Homo sapiens
-
spleen
-
 Homo sapiens
-
stomach
-
 Homo sapiens
-
testis
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + NAD+ 100% activity Homo sapiens ADP + NADP+
-
 ?
ATP + NADH 10% activity compared to NAD+ Homo sapiens ADP + NADPH
-
 ?
CTP + NAD+ 56% activity compared to ATP Homo sapiens CDP + NADP+
-
 ?
dATP + NAD+ 99% activity compared to ATP Homo sapiens dADP + NADP+
-
 ?
dITP + NAD+ 141% activity compared to ATP Homo sapiens dIDP + NADP+
-
 ?
GTP + NAD+ 162% activity compared to ATP Homo sapiens GDP + NADP+
-
 ?
hexametaphosphate + NAD+ 194% activity compared to ATP Homo sapiens ? + NADP+
-
 ?
ITP + NAD+ 104% activity compared to ATP Homo sapiens IDP + NADP+
-
 ?
metaphosphate + NAD+ 280% activity compared to ATP Homo sapiens ? + NADP+
-
 ?
additional information no activity with NAAD, ADP-ribose, adenosine, 5'-AMP, ADP, sphingosine, diacylglycerol, fructose 6-phosphate, trimetaphosphate, tripolyphosphate, phosphoenolpyruvate, and phosphocreatine Homo sapiens ?
-
 ?
tetrapolyphosphate + NAD+ 84% activity compared to ATP Homo sapiens tripolyphosphate + NADP+
-
 ?
TTP + NAD+ 87% activity compared to ATP Homo sapiens TDP + NADP+
-
 ?
UTP + NAD+ 41% activity compared to ATP Homo sapiens UDP + NADP+
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 43000, SDS-PAGE Homo sapiens
homodimer 2 * 43300, calculated from amino acid sequence Homo sapiens

Synonyms

Synonyms Comment Organism
C5orf33 protein
-
 Homo sapiens
NAD kinase
-
 Homo sapiens
NADK
-
 Homo sapiens