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Literature summary for 2.7.1.229 extracted from

  • Schimmel, S.; Hoffee, P.; Horecker, B.
    Deoxyribokinase from Salmonella typhimurium. Purification and properties (1974), Arch. Biochem. Biophys., 164, 560-570 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.229

Inhibitors

Inhibitors Comment Organism Structure
Li+
-
 Salmonella enterica subsp. enterica serovar Typhimurium
Na+
-
 Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 2-deoxy-D-ribose pH 7.5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
0.5
-
 ATP pH 7.5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

Metals/Ions Comment Organism Structure
additional information enzyme has an absolute requirement for divalent cations Salmonella enterica subsp. enterica serovar Typhimurium
NH4+ 0.5 M, 2.9fold increase in activity at pH 7.5 Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67800
-
 gel filtration Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification from deoxyribose-grown cells Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
70
-
 presence of 0.5 M NH4+, pH 7.5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-deoxy-D-ribitol
-
 Salmonella enterica subsp. enterica serovar Typhimurium ADP + 2-deoxy-D-ribitol 5-phosphate
-
 ?
ATP + 2-deoxy-D-ribose
-
 Salmonella enterica subsp. enterica serovar Typhimurium ADP + 2-deoxy-D-ribose 5-phosphate
-
 ?
ATP + D-ribose
-
 Salmonella enterica subsp. enterica serovar Typhimurium ADP + D-ribose 5-phosphate
-
 ?
dATP + 2-deoxy-D-ribose
-
 Salmonella enterica subsp. enterica serovar Typhimurium dADP + 2-deoxy-D-ribose 5-phosphate
-
 ?
dGTP + 2-deoxy-D-ribose
-
 Salmonella enterica subsp. enterica serovar Typhimurium dGDP + 2-deoxy-D-ribose 5-phosphate
-
 ?
GTP + 2-deoxy-D-ribose
-
 Salmonella enterica subsp. enterica serovar Typhimurium GDP + 2-deoxy-D-ribose 5-phosphate
-
 ?
ITP + 2-deoxy-D-ribose
-
 Salmonella enterica subsp. enterica serovar Typhimurium IDP + 2-deoxy-D-ribose 5-phosphate
-
 ?
additional information no substrates: D- and L-arabinose, D- and L-xylose, D-lyxose, D-glucose, D-galactose, D-mannose, D-altrose, D-fructose, D-erythrose, 2-deoxy-L-ribose, 2-deoxy-D-glucose, 2-deoxy-D-galactose, D-ribulose, sorbitol, xylitol, ribitol, L-arabitol, erythritol, glycerol, and beta-hydroxypropionaldehyde Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 33500, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8 7.2
-
 Salmonella enterica subsp. enterica serovar Typhimurium