Literature summary for 2.7.1.222 extracted from

Fricke, J.; Kargbo, R.; Regestein, L.; Lenz, C.; Peschel, G.; Rosenbaum, M.; Sherwood, A.; Hoffmeister, D.
Scalable hybrid synthetic/biocatalytic route to psilocybin (2020), Chemistry, 26, 8281-8285 .

Search for more literature for 2.7.1.222

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli KRX cells	Psilocybe cubensis

Protein Variants

Protein Variants	Comment	Organism
D224A	catalytically inactive	Psilocybe cubensis

Inhibitors

Inhibitors	Comment	Organism	Structure
Ca2+	the enzyme remains nearly inactive (less than 5%) with Ca2+	Psilocybe cubensis
Co2+	the enzyme shows clearly reduced activity (11.5%) with Co2+	Psilocybe cubensis
Cu2+	the enzyme remains nearly inactive (less than 5%) with Cu2+	Psilocybe cubensis
Mn2+	the enzyme remains nearly inactive (less than 5%) with Mn2+	Psilocybe cubensis
Ni2+	the enzyme remains nearly inactive (less than 5%) with Ni2+	Psilocybe cubensis
Zn2+	the enzyme remains nearly inactive (less than 5%) with Zn2+	Psilocybe cubensis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + 4-hydroxytryptamine	Psilocybe cubensis	-	ADP + norbaeocystin	-	?
ATP + psilocin	Psilocybe cubensis	-	ADP + psilocybin	-	?

Organism

Organism	UniProt	Comment	Textmining
Psilocybe cubensis	P0DPA8	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-Sepharose column chromatography	Psilocybe cubensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 4-hydroxytryptamine	-	Psilocybe cubensis	ADP + norbaeocystin	-	?
ATP + psilocin	-	Psilocybe cubensis	ADP + psilocybin	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 40400, calculated from amino acid sequence	Psilocybe cubensis

Synonyms

Synonyms	Comment	Organism
PsiK	-	Psilocybe cubensis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	Psilocybe cubensis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
4.5	-	4-hydroxytryptamine	at pH 7.0 and 40Â°C	Psilocybe cubensis
16.1	-	psilocin	at pH 7.0 and 40Â°C	Psilocybe cubensis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Psilocybe cubensis

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Release 2023.1 (January 2023)