Cloned (Comment) | Organism |
---|---|
gene NMRK1 | Homo sapiens |
gene NMRK1 | Mus musculus |
gene NMRK2 | Homo sapiens |
gene NMRK2 | Mus musculus |
gene NMRK2 | Danio rerio |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.068 | - |
1-(beta-D-ribofuranosyl)-nicotinamide | pH and temperature not specified in the publication, with GTP | Homo sapiens | |
0.088 | - |
1-(beta-D-ribofuranosyl)-nicotinamide | pH and temperature not specified in the publication, with ATP | Homo sapiens | |
0.19 | - |
1-(beta-D-ribofuranosyl)-nicotinamide | with ATP, pH and temperature not specified in the publication | Homo sapiens | |
30 | - |
1-(beta-D-ribofuranosyl)-nicotinamide | with GTP, pH and temperature not specified in the publication | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens | |
Mg2+ | required | Mus musculus | |
Mg2+ | required | Danio rerio |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-(beta-D-ribofuranosyl)-nicotinamide | Homo sapiens | - |
ADP + beta-nicotinamide D-ribonucleotide | - |
? | |
ATP + 1-(beta-D-ribofuranosyl)-nicotinamide | Mus musculus | - |
ADP + beta-nicotinamide D-ribonucleotide | - |
? | |
ATP + 1-(beta-D-ribofuranosyl)-nicotinamide | Danio rerio | - |
ADP + beta-nicotinamide D-ribonucleotide | - |
? | |
GTP + 1-(beta-D-ribofuranosyl)-nicotinamide | Homo sapiens | - |
GDP + beta-nicotinamide D-ribonucleotide | - |
? | |
GTP + 1-(beta-D-ribofuranosyl)-nicotinamide | Mus musculus | - |
GDP + beta-nicotinamide D-ribonucleotide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Danio rerio | F1QSN9 | - |
- |
Homo sapiens | Q9NPI5 | - |
- |
Homo sapiens | Q9NWW6 | - |
- |
Mus musculus | Q91W63 | - |
- |
Mus musculus | Q9D7C9 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brown adipose tissue | - |
Mus musculus | - |
kidney | - |
Mus musculus | - |
liver | - |
Mus musculus | - |
skeletal muscle | - |
Mus musculus | - |
skeletal muscle | high expression of NRK2 | Mus musculus | - |
spinal ganglion | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-(beta-D-ribofuranosyl)-nicotinamide | - |
Homo sapiens | ADP + beta-nicotinamide D-ribonucleotide | - |
? | |
ATP + 1-(beta-D-ribofuranosyl)-nicotinamide | - |
Mus musculus | ADP + beta-nicotinamide D-ribonucleotide | - |
? | |
ATP + 1-(beta-D-ribofuranosyl)-nicotinamide | - |
Danio rerio | ADP + beta-nicotinamide D-ribonucleotide | - |
? | |
GTP + 1-(beta-D-ribofuranosyl)-nicotinamide | - |
Homo sapiens | GDP + beta-nicotinamide D-ribonucleotide | - |
? | |
GTP + 1-(beta-D-ribofuranosyl)-nicotinamide | - |
Mus musculus | GDP + beta-nicotinamide D-ribonucleotide | - |
? | |
additional information | enzyme NRK2 highly prefers ATP, while isozyme NRK1 also uses GTP with similar activity | Homo sapiens | ? | - |
- |
|
additional information | enzyme NRK2 is restricted to ATP, while isozyme NRK1 also uses GTP | Mus musculus | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
More | see also EC 2.7.1.173 | Homo sapiens |
nicotinamide riboside kinase 1 | - |
Homo sapiens |
nicotinamide riboside kinase 1 | - |
Mus musculus |
nicotinamide riboside kinase 2 | - |
Homo sapiens |
nicotinamide riboside kinase 2 | - |
Mus musculus |
nicotinamide riboside kinase 2 | - |
Danio rerio |
NMRK1 | - |
Homo sapiens |
NMRK2 | - |
Homo sapiens |
NMRK2 | - |
Danio rerio |
NRK1 | - |
Homo sapiens |
NRK1 | - |
Mus musculus |
Nrk2 | - |
Homo sapiens |
Nrk2 | - |
Mus musculus |
Nrk2b | - |
Danio rerio |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.34 | - |
1-(beta-D-ribofuranosyl)-nicotinamide | pH and temperature not specified in the publication, with GTP | Homo sapiens | |
0.6 | - |
1-(beta-D-ribofuranosyl)-nicotinamide | pH and temperature not specified in the publication, with ATP | Homo sapiens | |
0.75 | - |
1-(beta-D-ribofuranosyl)-nicotinamide | with ATP, pH and temperature not specified in the publication | Homo sapiens | |
1.7 | - |
1-(beta-D-ribofuranosyl)-nicotinamide | with GTP, pH and temperature not specified in the publication | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens | |
ATP | - |
Mus musculus | |
ATP | - |
Danio rerio | |
GTP | - |
Homo sapiens | |
GTP | - |
Mus musculus |
Organism | Comment | Expression |
---|---|---|
Mus musculus | following injury to dorsal root ganglion neurons, Nmrk2 is the most upregulated NAD+ biosynthetic gene (by over 20fold). Mice with a loss of hexose-6-phosphate dehydrogenase (H6PDH) function, an ER-based enzyme required for local NADPH generation, manifest with severe muscle myopathy and on transcriptional analysis Nmrk2 expression is the most dysregulated gene (upregulated by over 60fold mRNA level). Nmrk2 mRNA expression is substantially induced (over 80fold) in models of lethal cardiomyopathy | up |
Homo sapiens | Nmrk2 mRNA expression is substantially induced (over 80fold) in models of lethal cardiomyopathy | up |
General Information | Comment | Organism |
---|---|---|
malfunction | a murine NRK1 loss-of-function model does not exhibit any gross phenotypic abnormalities, with steady state NAD+ levels unaffected, at least in the tissues that are examined (liver, skeletal muscle, brown adipose and kidney) | Mus musculus |
malfunction | a murine NRK2 loss-of-function model does not exhibit any gross phenotypic abnormalities, with steady state NAD+ levels unaffected, at least in the tissues that are examined (liver, skeletal muscle, brown adipose and kidney) | Mus musculus |
metabolism | the enzyme is involved in the NAD+ biosynthesis pathway. In the initial step of the pathway, NRK activity catalyses the phosphorylation of nicotinamide riboside (NR) to nicotinamide mononucleotide (NMN). Importance of different salvage pathways involved in metabolising the vitamin B3 class of NAD+ precursor molecules, with a particular focus on the nicotinamide riboside kinase pathway at both a tissue-specific and systemic level, overview | Danio rerio |
metabolism | the enzyme is involved in the NAD+ biosynthesis pathway. In the initial step of the pathway, NRK activity catalyses the phosphorylation of nicotinamide riboside (NR) to nicotinamide mononucleotide (NMN). Importance of different salvage pathways involved in metabolising the vitamin B3 class of NAD+ precursor molecules, with a particular focus on the nicotinamide riboside kinase pathway at both a tissue-specific and systemic level, regulation of the NRK enzymes, overview. Alternatively, NRK activity can phosphorylate nicotinic acid riboside (NaR) to nicotinic acid mononucleotide (NaMN), see for EC 2.7.1.173 | Homo sapiens |
metabolism | the enzyme is involved in the NAD+ biosynthesis pathway. In the initial step of the pathway, NRK activity catalyses the phosphorylation of nicotinamide riboside (NR) to nicotinamide mononucleotide (NMN). Importance of different salvage pathways involved in metabolising the vitamin B3 class of NAD+ precursor molecules, with a particular focus on the nicotinamide riboside kinase pathway at both a tissue-specific and systemic level, regulation of the NRK enzymes, overview. Alternatively, NRK activity can phosphorylate nicotinic acid riboside (NaR) to nicotinic acid mononucleotide (NaMN), see for EC 2.7.1.173 | Mus musculus |
additional information | proposed NRK expression in disease and potential therapeutic interventions | Homo sapiens |
additional information | proposed NRK expression in disease and potential therapeutic interventions | Mus musculus |
physiological function | although NRK1 and NRK2 do not appear critical in mice for endogenous NR salvage to NAD+, their activity has been determined essential for the utilisation of exogenous NR and, more surprisingly, NMN. Without expression of the NRK enzymes in tissues, the NAD+-boosting effects of nicotinamide riboside (NR) and NMN supplementation is blocked, whilst expression of alternative NAD+ biosynthesis enzymes remains comparable to wild-type mice. Phosphorylation of NR by NRK1 appears preferred to NRK2 even in skeletal muscle where Nmrk2 is specifically expressed and found at substantially higher mRNA levels than Nmrk1 | Mus musculus |
physiological function | although NRK1 and NRK2 do not appear critical in mice for endogenous NR salvage to NAD+, their activity has been determined essential for the utilisation of exogenous NR and, more surprisingly, NMN. Without expression of the NRK enzymes in tissues, the NAD+-boosting effects of nicotinamide riboside (NR) and NMN supplementation is blocked, whilst expression of alternative NAD+ biosynthesis enzymes remains comparable to wild-type mice. Phosphorylation of NR by NRK1 appears preferred to NRK2 even in skeletal muscle where Nmrk2 is specifically expressed and found at substantially higher mRNA levels than Nmrk1. In NAD+ deficiency, NRK2 may be induced to aid NAD+ biosynthesis. NRK2 appears to play a redundant role in NAD+ biosynthesis along with NRK1, at least in unchallenged models, its highly regulated expression particularly in times of stress suggest it may have role beyond NAD+ metabolism | Mus musculus |
physiological function | NRK2 appears to play a redundant role in NAD+ biosynthesis along with NRK1, at least in unchallenged models, its highly regulated expression particularly in times of stress suggest it may have role beyond NAD+ metabolism | Homo sapiens |
physiological function | NRK2 appears to play a redundant role in NAD+ biosynthesis along with NRK1, at least in unchallenged models, its highly regulated expression particularly in times of stress suggest it may have role beyond NAD+ metabolism | Danio rerio |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.057 | - |
1-(beta-D-ribofuranosyl)-nicotinamide | with GTP, pH and temperature not specified in the publication | Homo sapiens | |
3.95 | - |
1-(beta-D-ribofuranosyl)-nicotinamide | with ATP, pH and temperature not specified in the publication | Homo sapiens | |
5 | - |
1-(beta-D-ribofuranosyl)-nicotinamide | pH and temperature not specified in the publication, with GTP | Homo sapiens | |
6.82 | - |
1-(beta-D-ribofuranosyl)-nicotinamide | pH and temperature not specified in the publication, with ATP | Homo sapiens |