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Literature summary for 2.7.1.21 extracted from

  • Stepanenko, V.; Esipov, R.; Miroshnikov, A.; Andronova, V.; Galegov, G.; Yasko, M.; Guskova, A.; Skoblov, A.; Skoblov, Y.
    Cloning, expression, isolation, and properties of thymidine kinase from herpes simplex virus type 1, strain L2 (2011), Russ. J. Bioorg. Chem., 37, 436-440.
No PubMed abstract available
Search for more literature for 2.7.1.21

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli ER2566 cells Human alphaherpesvirus 1

Inhibitors

Inhibitors Comment Organism Structure
acyclovir H-phosphonate
-
 Human alphaherpesvirus 1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42192
-
 x * 42192, calculated from amino acid sequence Human alphaherpesvirus 1
43000
-
 x * 43000, SDS-PAGE Human alphaherpesvirus 1

Organism

Organism UniProt Comment Textmining
Human alphaherpesvirus 1
-
-
-
Human alphaherpesvirus 1 L2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and HiTrap Q column chromatography Human alphaherpesvirus 1

Storage Stability

Storage Stability Organism
-20°C, enzyme in buffer containing 250 mM imidazole, several months, without noticeable loss of activity Human alphaherpesvirus 1
4°C, enzyme in buffer containing 250 mM imidazole, 1 week, 50% loss of activity Human alphaherpesvirus 1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (E)-5-(2-bromovinyl)-2'-deoxyuridine
-
 Human alphaherpesvirus 1 ADP + (E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate
-
 ?
ATP + (E)-5-(2-bromovinyl)-2'-deoxyuridine
-
 Human alphaherpesvirus 1 L2 ADP + (E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate
-
 ?
ATP + 2',3'-dideoxycytidine
-
 Human alphaherpesvirus 1 ADP + 2',3'-dideoxycytidine 5'-phosphate
-
 ?
ATP + 2',3'-dideoxycytidine
-
 Human alphaherpesvirus 1 L2 ADP + 2',3'-dideoxycytidine 5'-phosphate
-
 ?
ATP + 2',3'-dideoxydehydrothymidine
-
 Human alphaherpesvirus 1 ADP + 2',3'-dideoxydehydrothymidine 5'-phosphate
-
 ?
ATP + 2',3'-dideoxydehydrothymidine
-
 Human alphaherpesvirus 1 L2 ADP + 2',3'-dideoxydehydrothymidine 5'-phosphate
-
 ?
ATP + 3'-deoxy-2',3'-didehydrothymidine
-
 Human alphaherpesvirus 1 ADP + 3'-deoxy-2',3'-didehydrothymidine 5'-phosphate
-
 ?
ATP + 3'-deoxythymidine
-
 Human alphaherpesvirus 1 ADP + 3'-deoxythymidine 5'-phosphate
-
 ?
ATP + 3'-fluoro-3'-deoxythymidine
-
 Human alphaherpesvirus 1 ADP + 3'-fluoro-3'-deoxythymidine 5'-phosphate
-
 ?
ATP + 9-(1,3-dihydroxy-2-propoxymethyl)guanine
-
 Human alphaherpesvirus 1 ADP + 9-(1,3-dihydroxy-2-propoxymethyl)guanine 5'-phosphate
-
 ?
ATP + 9-[(hydroxyethoxy)methyl]guanine
-
 Human alphaherpesvirus 1 ADP + 9-[(hydroxyethoxy)methyl]guanine 5'-phosphate
-
 ?
ATP + acyclovir
-
 Human alphaherpesvirus 1 ADP + acyclovir 5'-phosphate
-
 ?
ATP + beta-L-2',3'-dideoxy-3'-thiacytidine
-
 Human alphaherpesvirus 1 ADP + beta-L-2',3'-dideoxy-3'-thiacytidine 5'-phosphate
-
 ?
ATP + beta-L-2',3'-dideoxy-3'-thiacytidine
-
 Human alphaherpesvirus 1 L2 ADP + beta-L-2',3'-dideoxy-3'-thiacytidine 5'-phosphate
-
 ?
ATP + thymidine
-
 Human alphaherpesvirus 1 ADP + dTMP
-
 ?
ATP + thymidine
-
 Human alphaherpesvirus 1 L2 ADP + dTMP
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Human alphaherpesvirus 1
? x * 42192, calculated from amino acid sequence Human alphaherpesvirus 1

Synonyms

Synonyms Comment Organism
UL23
-
 Human alphaherpesvirus 1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5 for phosphorylation of thymidine Human alphaherpesvirus 1