Literature summary for 2.7.1.21 extracted from

Gardberg, A.; Shuvalova, L.; Monnerjahn, C.; Konrad, M.; Lavie, A.
Structural basis for the dual thymidine and thymidylate kinase activity of herpes thymidine kinases (2003), Structure, 11, 1265-1277.

Cloned(Commentary)

Cloned (Comment)	Organism
expression of the His-tagged, thrombin-cleavable full length or truncated enzyme in Escherichia coli strain BL21 C41 (DE3)	Herpes simplex virus type 4

Crystallization (Commentary)

Crystallization (Comment)	Organism
full length and truncated enzyme in complex with thymidine and ADP, or with thymidine, sulfate, and a bisubstrate analogues, or with TP4A, or with TP5A, hanging drop vapour diffusion method, reservoir solution containing 0.1 M MES, pH 6.5, 1.2 M ammonium sulfate, and 3 or 5% dioxane, dependent on the complex ligand, mixed with enzyme solution containing 5.5 mg/ml protein, 1.0 mM TMP, 2.0 mM ADP, 25 mM HEPES, pH 7.0, 5 mM MgSO4, 5 mM DTT, 100 mM KCl, and 5% glycerol, at equal volumes of 0.001 ml on a glass cover slide, X-ray diffraction structure determination and analysis at 2.0-3.0 A resolution, molecular replacement method	Herpes simplex virus type 4

Crystallization (Comment)

Organism

full length and truncated enzyme in complex with thymidine and ADP, or with thymidine, sulfate, and a bisubstrate analogues, or with TP4A, or with TP5A, hanging drop vapour diffusion method, reservoir solution containing 0.1 M MES, pH 6.5, 1.2 M ammonium sulfate, and 3 or 5% dioxane, dependent on the complex ligand, mixed with enzyme solution containing 5.5 mg/ml protein, 1.0 mM TMP, 2.0 mM ADP, 25 mM HEPES, pH 7.0, 5 mM MgSO4, 5 mM DTT, 100 mM KCl, and 5% glycerol, at equal volumes of 0.001 ml on a glass cover slide, X-ray diffraction structure determination and analysis at 2.0-3.0 A resolution, molecular replacement method

Herpes simplex virus type 4

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
36500	-	x * 38800, about, recombinant full length enzyme, amino acid sequence calculation, x * 36500, about, recombinant truncated enzyme, amino acid sequence calculation	Herpes simplex virus type 4
38800	-	x * 38800, about, recombinant full length enzyme, amino acid sequence calculation, x * 36500, about, recombinant truncated enzyme, amino acid sequence calculation	Herpes simplex virus type 4

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + thymidine	Herpes simplex virus type 4	-	ADP + thymidine 5'-phosphate	-	r
ATP + thymidine 5'-phosphate	Herpes simplex virus type 4	-	ADP + thymidine diphosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Herpes simplex virus type 4	-	type 4	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged, thrombin-cleavable full length or truncated enzyme from Escherichia coli strain BL21 C41 (DE3) by nickel affinity chromatography, cleavage by thrombin	Herpes simplex virus type 4

Reaction

Reaction	Comment	Organism	Reaction ID
ATP + thymidine = ADP + dTMP	substrate binding site structures, structural basis for substrate specificity and activity	Herpes simplex virus type 4	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
ATP + thymidine	-	Herpes simplex virus type 4	ADP + thymidine 5'-phosphate	-	r
ATP + thymidine 5'-phosphate	-	Herpes simplex virus type 4	ADP + thymidine diphosphate	-	r
additional information	wide substrate specificity phosphorylating pyrimidine nucleosides, nucleoside monophophates, and several nucleoside analogues	Herpes simplex virus type 4	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 38800, about, recombinant full length enzyme, amino acid sequence calculation, x * 36500, about, recombinant truncated enzyme, amino acid sequence calculation	Herpes simplex virus type 4

Synonyms

Synonyms	Comment	Organism
EHV4-TK	-	Herpes simplex virus type 4
thymidylate kinase	-	Herpes simplex virus type 4

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Herpes simplex virus type 4