Literature summary for 2.7.1.21 extracted from

Bird, L.E.; Ren, J.; Wright, A.; Leslie, K.D.; Degreve, B.; Balzarini, J.; Stammers, D.K.
Crystal structure of Varicella zoster virus thymidine kinase (2003), J. Biol. Chem., 278, 24680-24687.

Cloned(Commentary)

Cloned (Comment)	Organism
expression as GST-tagged enzyme in Escherichia coli strain BL21	Human alphaherpesvirus 3

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant GST-tagged enzyme complexed with ADP and E-5-(2-bromovinyl)-2'-deoxythymidine, sitting drop vapour diffusion method, 21°C, mixing of 0.001 ml of enzyme and reservoir solution, the enzyme solution contains 8 mg/ml protein, 0.4 mM ADP, 0.2 mM E-5-(2-bromovinyl)-2'-deoxythymidine, and 0.6 mM MgCl2, reservoir solution contains 0.1 M sodium acetate, at either pH 4.5 or 5.0, and 6-11% w/v PEG 20000, X-ray diffraction structure determination and analysis at 3.2 A resolution, molecular replacement method	Human alphaherpesvirus 3

Crystallization (Comment)

Organism

purified recombinant GST-tagged enzyme complexed with ADP and E-5-(2-bromovinyl)-2'-deoxythymidine, sitting drop vapour diffusion method, 21°C, mixing of 0.001 ml of enzyme and reservoir solution, the enzyme solution contains 8 mg/ml protein, 0.4 mM ADP, 0.2 mM E-5-(2-bromovinyl)-2'-deoxythymidine, and 0.6 mM MgCl2, reservoir solution contains 0.1 M sodium acetate, at either pH 4.5 or 5.0, and 6-11% w/v PEG 20000, X-ray diffraction structure determination and analysis at 3.2 A resolution, molecular replacement method

Human alphaherpesvirus 3

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
ATP + (E)-5-(2-bromovinyl)-2'-deoxyuridine	Human alphaherpesvirus 3	drug activation by phosphorylation	ADP + (E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate	-	?
ATP + thymidine	Human alphaherpesvirus 3	-	ADP + thymidine 5'-phosphate	-	?
additional information	Human alphaherpesvirus 3	the viral enzyme shows a broader substrate specificity than the human host cell enzyme	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Human alphaherpesvirus 3	-	i.e. human Herpes virus 3, VZV	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant GST-tagged enzyme from Escherichia coli strain BL21 by glutathione affinity chromatography	Human alphaherpesvirus 3

Reaction

Reaction	Comment	Organism	Reaction ID
ATP + thymidine = ADP + dTMP	active site structure, substrate binding structures	Human alphaherpesvirus 3	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
ATP + (E)-5-(2-bromovinyl)-2'-deoxyuridine	-	Human alphaherpesvirus 3	ADP + (E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate	-	?
ATP + (E)-5-(2-bromovinyl)-2'-deoxyuridine	drug activation by phosphorylation	Human alphaherpesvirus 3	ADP + (E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate	-	?
ATP + thymidine	-	Human alphaherpesvirus 3	ADP + thymidine 5'-phosphate	-	?
additional information	the viral enzyme shows a broader substrate specificity than the human host cell enzyme	Human alphaherpesvirus 3	?	-	?
additional information	modeling of ganciclovir binding into the enzyme crystal structure, overview	Human alphaherpesvirus 3	?	-	?

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Human alphaherpesvirus 3