Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | binding of ATP induces enzyme tetramerization, which leads to activation of the enzyme, the dimeric enzyme form is probably less active, 50-100% activation at up to 5 mM | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain B834(DE3), transient expression as Flag-tagged enzyme in LM-tk- cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
S13D | site-directed mutagenesis, ATP-induced tetramerization is perturbed, mutant is less activated by ATP and shows reduced activity compared to the wild-type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dTTP | competitive feedback inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00054 | - |
thymidine | pH 8.0, 37°C, recombinant wild-type enzyme | Homo sapiens | |
0.00596 | - |
thymidine | pH 8.0, 37°C, recombinant mutant S13D | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000, the enzyme exists as a dimer and as a tetramer | Homo sapiens |
50000 | - |
4 * 50000, the enzyme exists as a dimer and as a tetramer | Homo sapiens |
100000 | - |
recombinant enzyme, gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2'-deoxythymidine | Homo sapiens | - |
ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
ATP + thymidine | Homo sapiens | - |
ADP + thymidine 5'-phosphate | - |
? | |
additional information | Homo sapiens | the enzyme is tightly regulated in the cell cycle by multiple mechanism, e.g. by phosphorylation at Ser13 in mitotic-arrested cells, regulation model | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
enzyme contains a Val at position 106 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | phosphorylation at Ser13 has regulatory function | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain B834(DE3) by nickel affinity chromatography | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2'-deoxythymidine | - |
Homo sapiens | ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
ATP + thymidine | - |
Homo sapiens | ADP + thymidine 5'-phosphate | - |
? | |
additional information | the enzyme is tightly regulated in the cell cycle by multiple mechanism, e.g. by phosphorylation at Ser13 in mitotic-arrested cells, regulation model | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50000, the enzyme exists as a dimer and as a tetramer | Homo sapiens |
More | binding of ATP induces enzyme tetramerization, which may lead to activation of the enzyme, the dimeric enzyme form is probably less active | Homo sapiens |
tetramer | 4 * 50000, the enzyme exists as a dimer and as a tetramer | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
thymidine kinase | - |
Homo sapiens |
TK1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
265.5 | - |
thymidine | pH 8.0, 37°C, recombinant mutant S13D | Homo sapiens | |
282 | - |
thymidine | pH 8.0, 37°C, recombinant wild-type enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | binding of ATP induces enzyme tetramerization | Homo sapiens |