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Literature summary for 2.7.1.21 extracted from

  • Prota, A.; Vogt, J.; Pilger, B.; Perozzo, R.; Wurth, C.; Marquez, V.E.; Russ, P.; Schulz, G.E.; Folkers, G.; Scapozza, L.
    Kinetics and crystal structure of the wild-type and the engineered Y101F mutant of Herpes simplex virus type 1 thymidine kinase interacting with (North)-methanocarba-thymidine (2000), Biochemistry, 39, 9597-9603.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Herpes simplex virus

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting and hanging drop vapor diffusion method Herpes simplex virus

Protein Variants

Protein Variants Comment Organism
Y101F 12fold increased Km for thymidine Herpes simplex virus

Inhibitors

Inhibitors Comment Organism Structure
2'-exo-methanocarba-thymidine
-
Herpes simplex virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0002
-
thymidine pH 7.2, wild-type Herpes simplex virus
0.0025
-
thymidine pH 7.2, Y101F mutant Herpes simplex virus

Organism

Organism UniProt Comment Textmining
Herpes simplex virus
-
type 1
-

Purification (Commentary)

Purification (Comment) Organism
-
Herpes simplex virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2'-exo-methanocarba-thymidine MCT Herpes simplex virus ADP + 2'-exo-methanocarba-thymidine 5'-monophosphate
-
?
ATP + thymidine
-
Herpes simplex virus ADP + dTMP
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.011
-
2'-exo-methanocarba-thymidine pH 7.2, wild-type Herpes simplex virus
0.0515
-
2'-exo-methanocarba-thymidine pH 7.2, Y101F mutant Herpes simplex virus