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Literature summary for 2.7.1.21 extracted from

  • Kokoris, M.S.; Black, M.E.
    Characterization of herpes simplex virus type 1 thymidine kinase mutants engineered for improved ganciclovir or acyclovir activity (2002), Protein Sci., 11, 2267-2272.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.21

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00038
-
 thymidine 37°C, wild-type Herpes simplex virus
0.00095
-
 thymidine 37°C, mutant SR11 Herpes simplex virus
0.0013
-
 thymidine 37°C, mutant SR26 Herpes simplex virus
0.00264
-
 thymidine 37°C, mutant SR39 Herpes simplex virus
0.00333
-
 ganciclovir 37°C, mutant SR39 Herpes simplex virus
0.00337
-
 acyclovir 37°C, mutant SR26 Herpes simplex virus
0.0056
-
 acyclovir 37°C, mutant SR11 Herpes simplex virus
0.00614
-
 ganciclovir 37°C, mutant SR11 Herpes simplex virus
0.00979
-
 acyclovir 37°C, mutant SR39 Herpes simplex virus
0.01755
-
 ganciclovir 37°C, mutant SR26 Herpes simplex virus
0.0476
-
 ganciclovir 37°C, wild-type Herpes simplex virus
0.417
-
 acyclovir 37°C, wild-type Herpes simplex virus

Organism

Organism UniProt Comment Textmining
Herpes simplex virus
-
 type 1
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and 3 mutants, homogeneity Herpes simplex virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acyclovir
-
 Herpes simplex virus ?
-
 ?
ATP + ganciclovir
-
 Herpes simplex virus ?
-
 ?
ATP + thymidine
-
 Herpes simplex virus ADP + dTMP
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000283
-
 acyclovir 37°C, mutant SR26 Herpes simplex virus
0.000433
-
 acyclovir 37°C, mutant SR39 Herpes simplex virus
0.000467
-
 acyclovir 37°C, mutant SR11 Herpes simplex virus
0.0035
-
 ganciclovir 37°C, mutant SR39 Herpes simplex virus
0.0056
-
 ganciclovir 37°C, mutant SR11 Herpes simplex virus
0.0062
-
 thymidine 37°C, mutant SR26 Herpes simplex virus
0.007
-
 thymidine 37°C, mutant SR11 Herpes simplex virus
0.0089
-
 ganciclovir 37°C, mutant SR26 Herpes simplex virus
0.015
-
 acyclovir 37°C, wild-type Herpes simplex virus
0.017
-
 thymidine 37°C, mutant SR39 Herpes simplex virus
0.1
-
 ganciclovir 37°C, wild-type Herpes simplex virus
0.46
-
 thymidine 37°C, wild-type Herpes simplex virus